Antibodies Targeting Two Epitopes in SARS-CoV-2 Neutralize Pseudoviruses with the Spike Proteins from Different Variants
1
Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 97004, Taiwan
2
Department of Biochemistry, Tzu Chi University, Hualien 97004, Taiwan
3
Department of Laboratory Medicine, Buddhist Tzu Chi General Hospital, Hualien 97004, Taiwan
*
Author to whom correspondence should be addressed.
†
The first two authors contributed equally to this work.
Academic Editor: Sonia Zuñiga
Pathogens 2021, 10(7), 869; https://doi.org/10.3390/pathogens10070869
Received: 24 May 2021
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Revised: 7 July 2021
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Accepted: 7 July 2021
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Published: 9 July 2021
(This article belongs to the Collection SARS-CoV Infections)
The COVID-19 pandemic was caused by SARS-CoV-2 infection. To prevent the spread of SARS-CoV-2, an effective vaccine is required. Two linear peptides from potential B-cell epitopes in the spike protein of SARS-CoV-2 (a.a. 440–460; a.a. 494–506) were synthesized and used to immunize rabbits. High-titer antibodies of IgG were produced, purified, and verified by Western blot analysis. Antibodies against these two epitopes could effectively neutralize SARS-CoV-2 pseudoviral particles with the spike proteins from not only the original strain (basal; wild-type), but also a strain with a single point mutation (D614G), and two other emerging variants (the Alpha and Beta variants) prevalent around the world, but not from SARS-CoV. In conclusion, antibodies against these two epitopes are protective. This information is important for the development of vaccines against SARS-CoV-2.
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Keywords:
COVID-19; SARS-CoV-2; spike protein; epitope; D614G; Alpha variant; Beta variant; neutralization; pseudovirus
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MDPI and ACS Style
Yang, C.-H.; Li, H.-C.; Lee, W.-H.; Lo, S.-Y. Antibodies Targeting Two Epitopes in SARS-CoV-2 Neutralize Pseudoviruses with the Spike Proteins from Different Variants. Pathogens 2021, 10, 869. https://doi.org/10.3390/pathogens10070869
AMA Style
Yang C-H, Li H-C, Lee W-H, Lo S-Y. Antibodies Targeting Two Epitopes in SARS-CoV-2 Neutralize Pseudoviruses with the Spike Proteins from Different Variants. Pathogens. 2021; 10(7):869. https://doi.org/10.3390/pathogens10070869
Chicago/Turabian StyleYang, Chee-Hing, Hui-Chun Li, Wen-Han Lee, and Shih-Yen Lo. 2021. "Antibodies Targeting Two Epitopes in SARS-CoV-2 Neutralize Pseudoviruses with the Spike Proteins from Different Variants" Pathogens 10, no. 7: 869. https://doi.org/10.3390/pathogens10070869
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