Hemotropic Mycoplasma and Bartonella Species Diversity in Free-Roaming Canine and Feline from Luanda, Angola
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animal Recruitment and Data Collection
4.2. Nucleic Acid Extraction
4.3. PCR Amplification
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Agents | Target Gene | Primer Primers (5′–3′) | bp | PCR Conditions | Ref |
---|---|---|---|---|---|
Bartonella spp | ITS | P-bhenfa: TCTTCGTTTCTCTTTCTTCA P-benr1: CAAGCGCGCGCTCTAACC | 186/168 | 95 °C, 3 m; 35 cycles (94 °C, 15 s; 48 °C, 30 s; 72 °C, 30 s); 72 °C, 5 m | [43] |
Bartonella spp | ITS (n-PCR) | N-bhenf1a: GATGATCCCAAGCCTTCTGGC N-bhenr: AACCAACTGAGCTACAAGCC | 152/134 | 95 °C, 3 m; 35 cycles (94 °C, 15 s; 56 °C, 30 s; 72 °C, 30 s); 72 °C, 5 m | [43] |
CMhm, Mhf, CMt | 16S rRNA | F: ACGAAAGTCTGATGGAGCAATA R: ACGCCCAATAAATCCGRATAAT | 170/193 | 94 °C, 1 m; 35 cycles (94 °C, 1 m; 65 °C, 1 m; 72 °C, 30 s); 72 °C, 5 m | [44] |
CMt | 16S rRNA | F: AGAGGCGAAGGCGAAAACT R: CTACAACGCCGAAACACAAA | 138 | 95 °C, 2 m; 35 cycles (95 °C, 10 s; 58 °C, 30 s; 72 °C, 30 s); 72 °C 5 m | [45] |
Hemotropic mycoplasmas | 16S rRNA | F: ATACGGCCCATATTCCTACG R: TGCTCCACCACTTGTTCA | 595/618 | 95 °C, 3 m; 35 cycles (95 °C, 30 s; 60 °C, 30 s; 72 °C, 30 s); 72 °C 5 m | [46] |
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Mesquita, J.R.; Oliveira, A.C.; Neves, F.; Mendoza, J.R.; Luz, M.F.; Crespo, I.; dos Santos, T.F.; Santos-Silva, S.; Vilhena, H.; Barradas, P.F. Hemotropic Mycoplasma and Bartonella Species Diversity in Free-Roaming Canine and Feline from Luanda, Angola. Pathogens 2021, 10, 735. https://doi.org/10.3390/pathogens10060735
Mesquita JR, Oliveira AC, Neves F, Mendoza JR, Luz MF, Crespo I, dos Santos TF, Santos-Silva S, Vilhena H, Barradas PF. Hemotropic Mycoplasma and Bartonella Species Diversity in Free-Roaming Canine and Feline from Luanda, Angola. Pathogens. 2021; 10(6):735. https://doi.org/10.3390/pathogens10060735
Chicago/Turabian StyleMesquita, João R., Ana C. Oliveira, Frederico Neves, Jose R. Mendoza, Maria F. Luz, Inês Crespo, Thays F. dos Santos, Sérgio Santos-Silva, Hugo Vilhena, and Patrícia F. Barradas. 2021. "Hemotropic Mycoplasma and Bartonella Species Diversity in Free-Roaming Canine and Feline from Luanda, Angola" Pathogens 10, no. 6: 735. https://doi.org/10.3390/pathogens10060735