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Article

Toxocara canis and Toxocara cati Somatic and Excretory-Secretory Antigens Are Recognised by C-Type Lectin Receptors

1
Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hanover, Germany
2
Institute for Immunology, University of Veterinary Medicine Hannover, 30559 Hanover, Germany
3
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hanover, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Stefania Perrucci
Pathogens 2021, 10(3), 321; https://doi.org/10.3390/pathogens10030321
Received: 16 February 2021 / Revised: 1 March 2021 / Accepted: 2 March 2021 / Published: 9 March 2021
(This article belongs to the Collection Pathology and Parasitic Diseases of Animals)
Toxocara canis and Toxocara cati, the worldwide occurring intestinal roundworms of canids and felids, represent an important public health threat due to various disease manifestations in humans. Host recognition of pathogens is mediated by pattern recognition receptors (PRRs). Myeloid C-type lectin receptors (CLRs) are PRRs and recognise carbohydrate structures of various pathogens. As Toxocara excretory-secretory products (TES) are predominantly composed of glycoconjugates, they represent suitable targets for CLRs. However, the range of host-derived CLRs recognising Toxocara spp. is still unknown. Using a CLR-hFc fusion protein library, T. canis and T. cati L3 somatic antigens (TSOM) were bound by a variety of CLRs in enzyme-linked immunosorbent assay (ELISA), while their TES products interacted with macrophage galactose-type lectin-1 (MGL-1). Two prominent candidate CLRs, MGL-1 and macrophage C-type lectin (MCL), were selected for further binding studies. Immunofluorescence microscopy revealed binding of MGL-1 to the oral aperture of L3. Immunoblot experiments identified distinct protein fractions representing potential ligands for MGL-1 and MCL. To evaluate how these interactions influence the host immune response, bone marrow-derived dendritic cell (BMDC) assays were performed, showing MCL-dependent T. cati-mediated cytokine production. In conclusion, MGL-1 and MCL are promising candidates for immune modulation during Toxocara infection, deserving further investigation in the future. View Full-Text
Keywords: MGL-1; MCL; Dectin-1; PRR; immune evasion; toxocarosis; toxocariasis MGL-1; MCL; Dectin-1; PRR; immune evasion; toxocarosis; toxocariasis
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MDPI and ACS Style

Raulf, M.-K.; Lepenies, B.; Strube, C. Toxocara canis and Toxocara cati Somatic and Excretory-Secretory Antigens Are Recognised by C-Type Lectin Receptors. Pathogens 2021, 10, 321. https://doi.org/10.3390/pathogens10030321

AMA Style

Raulf M-K, Lepenies B, Strube C. Toxocara canis and Toxocara cati Somatic and Excretory-Secretory Antigens Are Recognised by C-Type Lectin Receptors. Pathogens. 2021; 10(3):321. https://doi.org/10.3390/pathogens10030321

Chicago/Turabian Style

Raulf, Marie-Kristin, Bernd Lepenies, and Christina Strube. 2021. "Toxocara canis and Toxocara cati Somatic and Excretory-Secretory Antigens Are Recognised by C-Type Lectin Receptors" Pathogens 10, no. 3: 321. https://doi.org/10.3390/pathogens10030321

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