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Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB
Open AccessArticle

An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome

1
Department of Medical Biology, Faculty of Medicine, University of Szeged, 6720 Szeged, Hungary
2
Department of Genetics, School of Medicine, Stanford University, Stanford, CA 94304, USA
3
Department of Biotechnology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
The first two authors contributed equally to this work.
Academic Editor: Barbara Klupp
Pathogens 2021, 10(2), 242; https://doi.org/10.3390/pathogens10020242
Received: 22 December 2020 / Revised: 17 February 2021 / Accepted: 18 February 2021 / Published: 20 February 2021
(This article belongs to the Special Issue Pseudorabies Virus Infections)
In the last couple of years, the implementation of long-read sequencing (LRS) technologies for transcriptome profiling has uncovered an extreme complexity of viral gene expression. In this study, we carried out a systematic analysis on the pseudorabies virus transcriptome by combining our current data obtained by using Pacific Biosciences Sequel and Oxford Nanopore Technologies MinION sequencing with our earlier data generated by other LRS and short-read sequencing techniques. As a result, we identified a number of novel genes, transcripts, and transcript isoforms, including splice and length variants, and also confirmed earlier annotated RNA molecules. One of the major findings of this study is the discovery of a large number of 5′-truncations of larger putative mRNAs being 3′-co-terminal with canonical mRNAs of PRV. A large fraction of these putative RNAs contain in-frame ATGs, which might initiate translation of N-terminally truncated polypeptides. Our analyses indicate that CTO-S, a replication origin-associated RNA molecule is expressed at an extremely high level. This study demonstrates that the PRV transcriptome is much more complex than previously appreciated. View Full-Text
Keywords: pseudorabies virus; herpesvirus; transcriptome; Pacific Biosciences; nanopore sequencing; long-read sequencing pseudorabies virus; herpesvirus; transcriptome; Pacific Biosciences; nanopore sequencing; long-read sequencing
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MDPI and ACS Style

Torma, G.; Tombácz, D.; Csabai, Z.; Göbhardter, D.; Deim, Z.; Snyder, M.; Boldogkői, Z. An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome. Pathogens 2021, 10, 242. https://doi.org/10.3390/pathogens10020242

AMA Style

Torma G, Tombácz D, Csabai Z, Göbhardter D, Deim Z, Snyder M, Boldogkői Z. An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome. Pathogens. 2021; 10(2):242. https://doi.org/10.3390/pathogens10020242

Chicago/Turabian Style

Torma, Gábor; Tombácz, Dóra; Csabai, Zsolt; Göbhardter, Dániel; Deim, Zoltán; Snyder, Michael; Boldogkői, Zsolt. 2021. "An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome" Pathogens 10, no. 2: 242. https://doi.org/10.3390/pathogens10020242

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