Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Primers
4.3. Plasmids
4.4. RNA Transcription and Electroporation
4.5. Antibodies and Reagents
4.6. Videomicroscopy
4.7. Immunofluorescence
4.8. APEX2 Labeling and Transmission Electron Microscopy (TEM)
4.9. Purification of Lipid Droplets
4.10. Quantification and Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Riva, L.; Spriet, C.; Barois, N.; Popescu, C.-I.; Dubuisson, J.; Rouillé, Y. Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants. Pathogens 2021, 10, 172. https://doi.org/10.3390/pathogens10020172
Riva L, Spriet C, Barois N, Popescu C-I, Dubuisson J, Rouillé Y. Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants. Pathogens. 2021; 10(2):172. https://doi.org/10.3390/pathogens10020172
Chicago/Turabian StyleRiva, Laura, Corentin Spriet, Nicolas Barois, Costin-Ioan Popescu, Jean Dubuisson, and Yves Rouillé. 2021. "Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants" Pathogens 10, no. 2: 172. https://doi.org/10.3390/pathogens10020172
APA StyleRiva, L., Spriet, C., Barois, N., Popescu, C.-I., Dubuisson, J., & Rouillé, Y. (2021). Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants. Pathogens, 10(2), 172. https://doi.org/10.3390/pathogens10020172