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Open AccessFeature PaperArticle

The Effect of ProHydrolase® on the Amino Acid and Intramuscular Anabolic Signaling Response to Resistance Exercise in Trained Males

1
Exercise and Nutrition Science Graduate Program, Lipscomb University, Nashville, TN 37204, USA
2
Central Instrument Facility, Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA
3
Exercise Science and Sport Management, Kennesaw State University, Kennesaw, GA 30144, USA
*
Author to whom correspondence should be addressed.
Sports 2020, 8(2), 13; https://doi.org/10.3390/sports8020013
Received: 19 December 2019 / Revised: 16 January 2020 / Accepted: 18 January 2020 / Published: 22 January 2020
(This article belongs to the Special Issue Nutrition Intervention in Exercise)
This double-blind study examined effects of a protease enzyme blend (Prohydrolase®) added to whey protein on post-resistance exercise aminoacidemia and intramuscular anabolic signaling were investigated in ten resistance-trained males. Participants completed 4 sets of 8–10 repetitions in the leg press and leg extension exercises at 75% of 1-repetition maximum. Participants then consumed either 250 mg of Prohydrolase® + 26 g of whey protein (PW), 26 g whey alone (W), or non-nutritive control (CON) in counterbalanced order. Blood samples were obtained prior to exercise (baseline) and then immediately-post (IP), 30-, 60-, 90-, 120-, and 180-min post-exercise. Muscle biopsies were taken at baseline, 1-h (1H), and 3-h (3H) post-exercise. Phosphorylation of AKTSer437 was decreased (3H only: p < 0.001), mTORSer2448 was increased (1H: p = 0.025; 3H: p = 0.009), and p70S6KThr412 remained unchanged similarly for each condition. Plasma leucine, branch-chained amino acids, and essential amino acid concentrations for PW were significantly higher than CON (p < 0.05) at 30 min and similar to W. Compared to IP, PW was the only treatment with elevated plasma leucine levels at 30 min (p = 0.007; ∆ = 57.8 mmol/L, 95% Confidence Interval (CI): 20.0, 95.6) and EAA levels at 180 min (p = 0.003; ∆ = 179.1 mmol/L, 95% CI: 77.5, 280.7). Area under the curve amino acid analysis revealed no differences between PW and W. While no different than W, these data indicate that PW was the only group to produce elevated amino acid concentrations 30-min and 180-min post-ingestion. View Full-Text
Keywords: protein supplementation; protease; muscle; leucine; protein synthesis protein supplementation; protease; muscle; leucine; protein synthesis
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MDPI and ACS Style

Townsend, J.R.; Morimune, J.E.; Jones, M.D.; Beuning, C.N.; Haase, A.A.; Boot, C.M.; Heffington, S.H.; Littlefield, L.A.; Henry, R.N.; Marshall, A.C.; VanDusseldorp, T.A.; Feito, Y.; Mangine, G.T. The Effect of ProHydrolase® on the Amino Acid and Intramuscular Anabolic Signaling Response to Resistance Exercise in Trained Males. Sports 2020, 8, 13. https://doi.org/10.3390/sports8020013

AMA Style

Townsend JR, Morimune JE, Jones MD, Beuning CN, Haase AA, Boot CM, Heffington SH, Littlefield LA, Henry RN, Marshall AC, VanDusseldorp TA, Feito Y, Mangine GT. The Effect of ProHydrolase® on the Amino Acid and Intramuscular Anabolic Signaling Response to Resistance Exercise in Trained Males. Sports. 2020; 8(2):13. https://doi.org/10.3390/sports8020013

Chicago/Turabian Style

Townsend, Jeremy R.; Morimune, Jaclyn E.; Jones, Megan D.; Beuning, Cheryle N.; Haase, Allison A.; Boot, Claudia M.; Heffington, Stephen H.; Littlefield, Laurel A.; Henry, Ruth N.; Marshall, Autumn C.; VanDusseldorp, Trisha A.; Feito, Yuri; Mangine, Gerald T. 2020. "The Effect of ProHydrolase® on the Amino Acid and Intramuscular Anabolic Signaling Response to Resistance Exercise in Trained Males" Sports 8, no. 2: 13. https://doi.org/10.3390/sports8020013

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