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Open AccessArticle

Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls

1
Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, China
2
Beijing Dairy Cattle Center, Qinghe South Town, Beijing 100085, China
3
Animal Genomics and Improvement Laboratory, BARC, USDA-ARS, BARC-East, Beltsville, MD 20705, USA
*
Authors to whom correspondence should be addressed.
Epigenomes 2019, 3(4), 21; https://doi.org/10.3390/epigenomes3040021
Received: 26 July 2019 / Revised: 1 September 2019 / Accepted: 17 September 2019 / Published: 26 September 2019
Semen quality is critical for fertility. However, it is easily influenced by environmental factors and can induce subfertility in the next generations. Here, we aimed to assess the impacts of differentially methylated regions and genes on semen quality and offspring fertility. A specific pair of monozygotic (MZ) twin artificial insemination (AI) Holstein bulls with moderately different sperm qualities (Bull1 > Bull2) was used in the study, and each twin bull had produced ~6000 recorded daughters nationwide in China. Using whole genome bisulfite sequencing, we profiled the landscape of the twin bulls’ sperm methylomes, and we observed markedly higher sperm methylation levels in Bull1 than in Bull2. Furthermore, we found 528 differentially methylated regions (DMR) between the MZ twin bulls, which spanned or overlapped with 309 differentially methylated genes (DMG). These DMG were particularly associated with embryo development, organ development, reproduction, and the nervous system. Several DMG were also shown to be differentially expressed in the sperm cells. Moreover, the significant differences in DNA methylation on gene INSL3 between the MZ twin bulls were confirmed at three different age points. Our results provided new insights into the impacts of AI bull sperm methylomes on offspring fertility. View Full-Text
Keywords: monozygotic twins; Holstein bull; DNA methylome; differentially methylated regions; differentially methylated genes monozygotic twins; Holstein bull; DNA methylome; differentially methylated regions; differentially methylated genes
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MDPI and ACS Style

Liu, S.; Chen, S.; Cai, W.; Yin, H.; Liu, A.; Li, Y.; Liu, G.E.; Wang, Y.; Yu, Y.; Zhang, S. Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls. Epigenomes 2019, 3, 21. https://doi.org/10.3390/epigenomes3040021

AMA Style

Liu S, Chen S, Cai W, Yin H, Liu A, Li Y, Liu GE, Wang Y, Yu Y, Zhang S. Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls. Epigenomes. 2019; 3(4):21. https://doi.org/10.3390/epigenomes3040021

Chicago/Turabian Style

Liu, Shuli; Chen, Siqian; Cai, Wentao; Yin, Hongwei; Liu, Aoxing; Li, Yanhua; Liu, George E.; Wang, Yachun; Yu, Ying; Zhang, Shengli. 2019. "Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls" Epigenomes 3, no. 4: 21. https://doi.org/10.3390/epigenomes3040021

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