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Epigenomes 2017, 1(1), 6;

The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences

Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Author to whom correspondence should be addressed.
Academic Editor: Etienne Bucher
Received: 28 February 2017 / Revised: 5 May 2017 / Accepted: 11 May 2017 / Published: 18 May 2017
(This article belongs to the Special Issue Plant Epigenome Dynamics)
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The spontaneous emergence among common pea (Pisum sativum L.) cultivars of off-type rogue plants exhibiting leaves with narrower and pointed leaflets and stipules and the non-Mendelian inheritance of this new phenotype were first described in the early 20th century. However, so far, no studies at the molecular level of this first identified case of paramutation have been carried out. In this study, we show for the first time that the pea rogue paramutation is accompanied by alterations in the methylation status of specific genomic sequences. Although, no significant differences were observed in the genome-wide DNA methylation in leaves of non-rogue cv. Onward in comparison to its rogue paramutant line JI2723, 22 DNA sequences were identified by methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) analysis as differentially methylated in the two epigenomes. Mitotically inherited through all leaf tissues, the differential methylation patterns were also found to be meiotically inherited and conserved in pollen grains for 12 out of the 22 sequences. Fourteen of the sequences were successfully amplified in cDNA but none of them exhibited significant differential expression in the two contrasting epigenotypes. The further exploitation of the present research results on the way towards the elucidation of the molecular mechanisms behind this interesting epigenetic phenomenon is discussed. View Full-Text
Keywords: paramutation; epigenetics; rogue plants; Pisum sativum; DNA methylation paramutation; epigenetics; rogue plants; Pisum sativum; DNA methylation

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Santo, T.E.; Pereira, R.J.; Leitão, J.M. The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences. Epigenomes 2017, 1, 6.

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