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The Development of Cytogenetic Maps for Malaria Mosquitoes

1
Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, Russia
2
Department of Entomology, Fralin Life Science Institute, Institute and State University, Blacksburg, VA 24060, USA
*
Author to whom correspondence should be addressed.
Insects 2018, 9(3), 121; https://doi.org/10.3390/insects9030121
Received: 15 August 2018 / Revised: 13 September 2018 / Accepted: 13 September 2018 / Published: 17 September 2018
(This article belongs to the Special Issue Integrative Mosquito Biology: From Molecules to Ecosystems)
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Abstract

Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi. View Full-Text
Keywords: cytogenetic map; chromosome map; malaria mosquitoes; Anopheles; polytene chromosomes; Anopheles beklemishevi; chromosome map development; photomap; high-pressure technique; chromosome straightening cytogenetic map; chromosome map; malaria mosquitoes; Anopheles; polytene chromosomes; Anopheles beklemishevi; chromosome map development; photomap; high-pressure technique; chromosome straightening
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Artemov, G.N.; Stegniy, V.N.; Sharakhova, M.V.; Sharakhov, I.V. The Development of Cytogenetic Maps for Malaria Mosquitoes. Insects 2018, 9, 121.

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