Regulation of Juvenile Hormone on Summer Diapause of Geleruca daurica and Its Pathway Analysis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Rearing
2.2. JHA Treatment
2.3. RNA Extraction and cDNA Synthesis
2.4. Transcriptome Sequencing, Assembly and Bioinformatics Analysis
2.5. Quantitative Real-Time PCR
2.6. Total Lipid Content Measurement
2.7. Behavioral and Developmental Observation
2.8. Statistical Analysis
3. Results
3.1. Effects of JHA on the Development of G. daurica Adults
3.2. Effects of JHA on the Total Lipid Content in G. daurica
3.3. Transcriptome Changes in Response to JHA Application
3.4. Effects of JHA Application on JH Signaling Pathway-Related Gene Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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DEG_ID | Cka vs. Ta | CKb vs. Tb | Nr_Annotation | ||||
---|---|---|---|---|---|---|---|
FDR | Log2FC | Regulated | FDR | Log2FC | Regulated | ||
c85279.graph_c0 | 2.37 × 10−13 | 2.575 | up | 2.92 × 10−2 | 1.303 | up | vitellogenin-like |
c82613.graph_c0 | 6.25 × 10−6 | 1.369 | up | 1.19 × 10−3 | 1.380 | up | fatty acid synthase-like |
c74827.graph_c0 | 3.39 × 10−3 | 1.336 | up | 5.83 × 10−3 | 1.558 | up | uncharacterized protein LOC111513065 |
c71191.graph_c0 | 1.61 × 10−5 | 1.272 | up | 4.84 × 10−3 | 1.228 | up | probable pseudouridine-5'-phosphatase |
c69643.graph_c0 | 8.60 × 10−3 | −1.007 | down | 7.39 × 10−4 | −1.295 | down | retinoid-inducible serine carboxypeptidase-like |
c37561.graph_c0 | 2.11 × 10−6 | −1.034 | down | 1.46 × 10−9 | −1.617 | down | PREDICTED: crustapain-like |
c64926.graph_c0 | 5.25 × 10−3 | −1.065 | down | 7.01 × 10−11 | −1.777 | down | -- |
c84589.graph_c0 | 9.74 × 10−5 | −1.160 | down | 1.64 × 10−5 | −1.526 | down | protein msta-like |
c72112.graph_c0 | 1.80 × 10−5 | −1.360 | down | 6.99 × 10−30 | −2.327 | down | facilitated trehalose transporter Tret1-like |
c73343.graph_c0 | 2.44 × 10−3 | −1.365 | down | 4.20 × 10−2 | −1.330 | down | -- |
c82886.graph_c0 | 3.61 × 10−6 | −1.401 | down | 4.31 × 10−2 | −1.235 | down | nose resistant to fluoxetine protein 6-like isoform X1 |
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Ma, H.-Y.; Li, Y.-Y.; Li, L.; Tan, Y.; Pang, B.-P. Regulation of Juvenile Hormone on Summer Diapause of Geleruca daurica and Its Pathway Analysis. Insects 2021, 12, 237. https://doi.org/10.3390/insects12030237
Ma H-Y, Li Y-Y, Li L, Tan Y, Pang B-P. Regulation of Juvenile Hormone on Summer Diapause of Geleruca daurica and Its Pathway Analysis. Insects. 2021; 12(3):237. https://doi.org/10.3390/insects12030237
Chicago/Turabian StyleMa, Hong-Yue, Yan-Yan Li, Ling Li, Yao Tan, and Bao-Ping Pang. 2021. "Regulation of Juvenile Hormone on Summer Diapause of Geleruca daurica and Its Pathway Analysis" Insects 12, no. 3: 237. https://doi.org/10.3390/insects12030237