Next Article in Journal
Insecticide Resistance Monitoring in Field Populations of the Whitebacked Planthopper Sogatella furcifera (Horvath) in China, 2019–2020
Previous Article in Journal
Comparing Light—Emitting—Diodes Light Traps for Catching Anopheles Mosquitoes in a Forest Setting, Western Thailand
Article

Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

1
Biology Centre CAS, Institute of Entomology, 370 05 České Budějovice, Czech Republic
2
Department of Genetics and Agricultural Biotechnology, Faculty of Agriculture, University of South Bohemia, 370 05 České Budějovice, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Jørgen Eilenberg
Insects 2021, 12(12), 1077; https://doi.org/10.3390/insects12121077
Received: 19 October 2021 / Revised: 11 November 2021 / Accepted: 29 November 2021 / Published: 30 November 2021
(This article belongs to the Section Insect Pest and Vector Management)
The Colorado potato beetle is a serious insect pest, attacking mainly potato. This pest causes severe yield loss all over the world and it is difficult to control by chemical pesticides because it quickly develops resistance to them. In our study we investigated the potential of the fungus Beauveria bassiana, a natural pathogen of insects, to kill adults of the Colorado potato beetle. The novelty of this study is that strains of the fungus were isolated from naturally infected adults of the pest which were collected in potato fields in the Czech Republic. A suspension of B. bassiana spores was applied to test beetles and their survival was observed under constant conditions. Obtained results revealed that some strains of the fungus were able to kill almost all treated beetles in 21 days and can be therefore recommended for the development of a new biopesticide. The results of this study can thus be applied in effective biological control of the most serious pest of potato.
The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a serious, widely distributed pest of potato and other crops. This pest is able to defoliate the host plant and cause severe yield loss. Moreover, the pest quickly becomes resistant to many chemical pesticides. Therefore, the development of novel biopesticides targeting this pest is urgently needed. The purpose of this study was to obtain new strains of the entomopathogenic fungus Beauveria bassiana and assess their efficacy against L. decemlineata adults under laboratory conditions. Twelve strains were isolated from cadavers of Colorado potato beetles collected in potato fields in the Czech Republic. Test beetles were treated by suspensions of conidia at the concentration of 1 × 107 spores per milliliter and their survival was recorded daily for three weeks. The results of the bioassays revealed that all new native strains were pathogenic to L. decemlineata adults and caused mortality up to 100% at the end of the trial period with an LT50 of about 7 days. These strains were more virulent than a reference strain GHA and some of them can be recommended for the development of a new mycoinsecticide against L. decemlineata. Our findings also highlight the importance of searching for perspective strains of entomopathogenic fungi among naturally infected hosts. View Full-Text
Keywords: potato; insect pest; entomopathogenic fungi; biological control; efficacy; mycoinsecticides potato; insect pest; entomopathogenic fungi; biological control; efficacy; mycoinsecticides
Show Figures

Figure 1

MDPI and ACS Style

Zemek, R.; Konopická, J.; Jozová, E.; Skoková Habuštová, O. Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata. Insects 2021, 12, 1077. https://doi.org/10.3390/insects12121077

AMA Style

Zemek R, Konopická J, Jozová E, Skoková Habuštová O. Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata. Insects. 2021; 12(12):1077. https://doi.org/10.3390/insects12121077

Chicago/Turabian Style

Zemek, Rostislav, Jana Konopická, Eva Jozová, and Oxana Skoková Habuštová. 2021. "Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata" Insects 12, no. 12: 1077. https://doi.org/10.3390/insects12121077

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop