Next Article in Journal
Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes
Previous Article in Journal
Monitoring Dermacentor reticulatus Host-Seeking Activity in Natural Conditions
Open AccessArticle

Lysine Acetyltransferase p300/CBP Plays an Important Role in Reproduction, Embryogenesis and Longevity of the Pea Aphid Acyrthosiphon pisum

1
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, Ohlebergsweg 12, 35392 Giessen, Germany
2
Institute for Insect Biotechnology, Justus-Liebig University of Giessen, Heinrich-Buff-Ring 26–32, 35392 Giessen, Germany
*
Author to whom correspondence should be addressed.
Insects 2020, 11(5), 265; https://doi.org/10.3390/insects11050265
Received: 27 March 2020 / Revised: 16 April 2020 / Accepted: 22 April 2020 / Published: 26 April 2020
CREB-binding protein (p300/CBP) is a universal transcriptional co-regulator with lysine acetyltransferase activity. Drosophila melanogaster p300/CBP is a well-known regulator of embryogenesis, and recent studies in beetles and cockroaches have revealed the importance of this protein during post-embryonic development and endocrine signaling. In pest insects, p300/CBP may therefore offer a useful target for control methods based on RNA interference (RNAi). We investigated the role of p300/CBP in the pea aphid (Acyrthosiphon pisum), a notorious pest insect used as a laboratory model for the analysis of complex life-history traits. The RNAi-based attenuation of A. pisum p300/CBP significantly reduced the aphid lifespan and number of offspring, as well as shortening the reproductive phase, suggesting the manipulation of this gene contributes to accelerated senescence. Furthermore, injection of p300/CBP dsRNA also reduced the number of viable offspring and increased the number of premature nymphs, which developed in abnormally structured ovaries. Our data confirm the evolutionarily conserved function of p300/CBP during insect embryogenesis and show that the protein has a critical effect on longevity, reproduction and development in A. pisum. The potent effect of p300/CBP silencing indicates that this regulatory protein is an ideal target for RNAi-based aphid control. View Full-Text
Keywords: KAT3; CREB-binding protein; RNA interference; senescence; life-history traits; nejire KAT3; CREB-binding protein; RNA interference; senescence; life-history traits; nejire
Show Figures

Figure 1

MDPI and ACS Style

Kirfel, P.; Vilcinskas, A.; Skaljac, M. Lysine Acetyltransferase p300/CBP Plays an Important Role in Reproduction, Embryogenesis and Longevity of the Pea Aphid Acyrthosiphon pisum. Insects 2020, 11, 265.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop