Next Article in Journal
Ingestion of Novaluron Elicits Transovarial Activity in Stephanitis pyrioides (Hemiptera: Tingidae)
Next Article in Special Issue
DNA Metabarcoding as a Tool for Disentangling Food Webs in Agroecosystems
Previous Article in Journal
A Review of the Phenotypic Traits Associated with Insect Dispersal Polymorphism, and Experimental Designs for Sorting out Resident and Disperser Phenotypes
Open AccessArticle

Use of Molecular Gut Content Analysis to Decipher the Range of Food Plants of the Invasive Spotted Lanternfly, Lycorma delicatula

Department of Entomology, University of Maryland, College Park, MD 20742, USA
*
Author to whom correspondence should be addressed.
Insects 2020, 11(4), 215; https://doi.org/10.3390/insects11040215
Received: 14 February 2020 / Revised: 25 March 2020 / Accepted: 26 March 2020 / Published: 1 April 2020
Spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), is an introduced highly invasive insect pest in the US that poses a significant risk to forestry and agriculture. Assessing and predicting plant usage of the lanternfly has been challenging, and little is known regarding the lanternfly nymph association with its host plants. In this study, we focused on: (a) providing a protocol for using molecular markers for food plant identification of L. delicatula; (b) determining whether the ingested plant DNA corresponds with DNA of the plants from which the lanternfly was collected; and, (c) investigating the spectrum of ingested plants. We utilized gut contents of third and fourth instar nymphs that were collected from multiple plants; we isolated ingested plant DNA and identified consumed plants. We demonstrated that (a) up to 534 bp of the rbcL gene from ingested plants can be detected in L. delicatula guts, (b) ingested plants in ~93% of the nymphs did not correspond with the plants from which the nymphs were collected, and (c) both introduced and native plants, as well as woody and non-woody plants, were ingested. This information will aid effective the monitoring and management of the lanternfly, as well as predict the lanternfly host plants with range expansion. View Full-Text
Keywords: insect gut content; invasive species; Lycorma delicatula; plant DNA barcoding; trophic interactions insect gut content; invasive species; Lycorma delicatula; plant DNA barcoding; trophic interactions
Show Figures

Graphical abstract

MDPI and ACS Style

Avanesyan, A.; Lamp, W.O. Use of Molecular Gut Content Analysis to Decipher the Range of Food Plants of the Invasive Spotted Lanternfly, Lycorma delicatula. Insects 2020, 11, 215.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop