Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insects and Plant Culture
2.2. Protein Purification
2.3. Bioassays with Elicitor Protein PeBC1
2.4. Isolation of RNA and cDNA Synthesis
2.5. Reverse Transcription–Quantitative PCR (RT-qPCR)
2.6. Statistical Analysis
3. Results
3.1. Effect of Elicitor PeBC1 on Nymphal Development Time of Aphids
3.2. Effect of Elicitor PeBC1 on the Fecundity of Aphids
3.3. Expression Levels of JA and SA Pathway-Associated Genes in Response to PeBC1 Elicitor
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Basit, A.; Hanan, A.; Nazir, T.; Majeed, M.Z.; Qiu, D. Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.). Insects 2019, 10, 35. https://doi.org/10.3390/insects10020035
Basit A, Hanan A, Nazir T, Majeed MZ, Qiu D. Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.). Insects. 2019; 10(2):35. https://doi.org/10.3390/insects10020035
Chicago/Turabian StyleBasit, Abdul, Abdul Hanan, Talha Nazir, Muhammad Zeeshan Majeed, and Dewen Qiu. 2019. "Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)" Insects 10, no. 2: 35. https://doi.org/10.3390/insects10020035