Next Article in Journal
Survival of Ectoparasitic Mites Tropilaelaps mercedesae in Association with Honeybee Hive Products
Previous Article in Journal
Limited Cross Plant Movement and Non-Crop Preferences Reduce the Efficiency of Honey Bees as Pollinators of Hybrid Carrot Seed Crops
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Insects 2019, 10(2), 35; https://doi.org/10.3390/insects10020035

Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)

1
Key Laboratory of Integrated Pest Management in Crops, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Department of Entomology, College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan
*
Author to whom correspondence should be addressed.
Received: 3 December 2018 / Revised: 15 January 2019 / Accepted: 22 January 2019 / Published: 24 January 2019
  |  
PDF [2081 KB, uploaded 24 January 2019]
  |  

Abstract

Elicitors are biofactors that induce resistance in plants against different insect pests. This in vitro study evaluated the impact of a novel elicitor protein PeBC1, extracted from a necrotrophic fungus Botrytis cinerea, on the development and fecundity parameters of green peach aphid (Myzus persicae) on common beans (Phaseolus vulgaris L.). Three different concentrations of PeBC1 elicitor (i.e., 33.56, 25.43, 19.33 µg mL−1) were applied at three different temperature regimes (i.e., 18, 21, and 25 °C). Elicitor treatments were applied topically on the bean plants at 3-leaf stage and newly emerged (0–6 h old) apterous adult aphids were exposed to these treated leaves. In addition to the biological parameters of aphids, the relative expression levels of key genes associated with jasmonic acid (JA) and salicylic acid (SA) plant defense pathways were also determined through RT-qPCR. Results of bioassays revealed that the application of PeBC1 elicitor protein exhibited pronounced and significant (p < 0.05) sub-lethal effects on green peach aphids. The fecundity was reduced and the nymphal development time was prolonged by different concentrations of PeBC1 elicitor and temperature regimes. Gene expression studies showed that the exogenous application of PeBC1 induced a significant upregulation of the expression levels of JA and SA pathway-associated genes in bean plants. As compared to control, elicitor-treated plants exhibited an induced resistance against aphids. Our findings suggest the potential use of PeBC1 elicitor protein in future bio-intensive management strategies against sap-sucking insect pests such as green peach aphids. View Full-Text
Keywords: elicitor proteins; PeBC1; Myzus persicae; life-history traits; jasmonic acid pathway; salicylic acid pathway elicitor proteins; PeBC1; Myzus persicae; life-history traits; jasmonic acid pathway; salicylic acid pathway
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Basit, A.; Hanan, A.; Nazir, T.; Majeed, M.Z.; Qiu, D. Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.). Insects 2019, 10, 35.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Insects EISSN 2075-4450 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top