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Open AccessArticle

Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)

1
Key Laboratory of Integrated Pest Management in Crops, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Department of Entomology, College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan
*
Author to whom correspondence should be addressed.
Insects 2019, 10(2), 35; https://doi.org/10.3390/insects10020035
Received: 3 December 2018 / Revised: 15 January 2019 / Accepted: 22 January 2019 / Published: 24 January 2019
Elicitors are biofactors that induce resistance in plants against different insect pests. This in vitro study evaluated the impact of a novel elicitor protein PeBC1, extracted from a necrotrophic fungus Botrytis cinerea, on the development and fecundity parameters of green peach aphid (Myzus persicae) on common beans (Phaseolus vulgaris L.). Three different concentrations of PeBC1 elicitor (i.e., 33.56, 25.43, 19.33 µg mL−1) were applied at three different temperature regimes (i.e., 18, 21, and 25 °C). Elicitor treatments were applied topically on the bean plants at 3-leaf stage and newly emerged (0–6 h old) apterous adult aphids were exposed to these treated leaves. In addition to the biological parameters of aphids, the relative expression levels of key genes associated with jasmonic acid (JA) and salicylic acid (SA) plant defense pathways were also determined through RT-qPCR. Results of bioassays revealed that the application of PeBC1 elicitor protein exhibited pronounced and significant (p < 0.05) sub-lethal effects on green peach aphids. The fecundity was reduced and the nymphal development time was prolonged by different concentrations of PeBC1 elicitor and temperature regimes. Gene expression studies showed that the exogenous application of PeBC1 induced a significant upregulation of the expression levels of JA and SA pathway-associated genes in bean plants. As compared to control, elicitor-treated plants exhibited an induced resistance against aphids. Our findings suggest the potential use of PeBC1 elicitor protein in future bio-intensive management strategies against sap-sucking insect pests such as green peach aphids. View Full-Text
Keywords: elicitor proteins; PeBC1; Myzus persicae; life-history traits; jasmonic acid pathway; salicylic acid pathway elicitor proteins; PeBC1; Myzus persicae; life-history traits; jasmonic acid pathway; salicylic acid pathway
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MDPI and ACS Style

Basit, A.; Hanan, A.; Nazir, T.; Majeed, M.Z.; Qiu, D. Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.). Insects 2019, 10, 35. https://doi.org/10.3390/insects10020035

AMA Style

Basit A, Hanan A, Nazir T, Majeed MZ, Qiu D. Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.). Insects. 2019; 10(2):35. https://doi.org/10.3390/insects10020035

Chicago/Turabian Style

Basit, Abdul; Hanan, Abdul; Nazir, Talha; Majeed, Muhammad Z.; Qiu, Dewen. 2019. "Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)" Insects 10, no. 2: 35. https://doi.org/10.3390/insects10020035

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