A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Phylogenetic Analyses
2.2. Animals and Preparation of Antennae
2.3. Reverse Transcription PCR Amplification for Generating Riboprobes
2.4. Synthesis of Riboprobes for In Situ Hybridization
2.5. Fluorescence In Situ Hybridization (FISH)
2.6. RNA Extraction and Reverse Transcription PCR for Comparing SgreOR Expression in Males Versus Females
3. Results
3.1. Categorization of SgreORs Based on Phylogenetic Analyses
3.2. Expression of SgreORs and SNMP1
3.3. Expression of Group II ORs Co-Expressed with SNMP1 in Males and Females
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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SgreOR Group | Co-Expressed with SNMP1 | Not Co-Expressed with SNMP1 | ||||||
---|---|---|---|---|---|---|---|---|
Group I (9 ORs) 8 ORs examined | OR2 OR8 | OR3 OR9 | OR5 | OR6 | OR4 | OR7 | ||
Group II (38 ORs) 33 ORs examined | OR84 OR90 OR94 OR101 OR109 OR113 | OR85 OR91 OR95 OR102 OR110 OR114 | OR86 OR92 OR96 OR103 OR111 OR116 | OR88 OR93 OR97 OR108 OR112 OR118 | OR82 OR98 OR106 | OR83 OR99 | OR87 OR100 | OR89 OR105 |
Group III (70 ORs) 42 ORs examined | OR22 | OR23 | OR38 | OR11 OR16 OR27 OR31 OR35 OR41 OR49 OR54 OR66 OR71 | OR13 OR17 OR28 OR32 OR37 OR43 OR51 OR57 OR67 OR76 | OR14 OR25 OR29 OR33 OR39 OR45 OR52 OR61 OR68 OR80 | OR15 OR26 OR30 OR34 OR40 OR47 OR53 OR62 OR70 |
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Pregitzer, P.; Jiang, X.; Lemke, R.-S.; Krieger, J.; Fleischer, J.; Breer, H. A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1. Insects 2019, 10, 350. https://doi.org/10.3390/insects10100350
Pregitzer P, Jiang X, Lemke R-S, Krieger J, Fleischer J, Breer H. A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1. Insects. 2019; 10(10):350. https://doi.org/10.3390/insects10100350
Chicago/Turabian StylePregitzer, Pablo, Xingcong Jiang, René-Sebastian Lemke, Jürgen Krieger, Jörg Fleischer, and Heinz Breer. 2019. "A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1" Insects 10, no. 10: 350. https://doi.org/10.3390/insects10100350