Serum LH Level on the Day of hCG Administration as a Predictor of the Reproductive Outcomes in Ovulation Induction Cycle Frozen–Thawed Embryo Transfer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Population
2.2. Serum Hormonal and Ultrasound Assays
2.3. Treatment Protocol
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
LH | Luteinizing hormone |
FET | Frozen embryo transfer |
OI | Ovulation induction |
hCG | Human chorionic gonadotropin |
FSH | Follicle-stimulating hormone |
E2 | Estradiol |
AFC | Antral follicle count |
ART | Assisted reproductive technology |
PGD/PGS | Pre-implantation genetic diagnosis/screening |
OHSS | Ovarian hyperstimulation syndrome |
GnRH | Gonadotropin-releasing hormone |
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Clinical Parameters | Frozen-Thawed Enbryo Transfer (n = 250) |
---|---|
Age of woman (yr) | 34.29 ± 5.66 |
LH on hCG day (mIU/mL) | 18.9 ± 23.86 |
Endometrial thickness (mm) | 8.4 ± 1.58 |
BMI (kg/m2) | 22.8 ± 2.90 |
AFC (#) | 13.8 ± 6.47 |
Basal FSH (MIU/mL) | 7.98 ± 3.23 |
Basal LH (MIU/mL) | 4.87 ± 2.95 |
Basal E2 (pg/nL) | 50.33 ± 32.67 |
Duration of infertility(yr) | 4.37 ± 3.62 |
E2 on hCG day (pg/mL) | 717.31 ± 549.55 |
No. of embryos transferred | 1.74 ± 0.44 |
Tine of HMG(d) | 9.41 ± 5.52 |
No. of total retrieved oocytes | 11 ± 6.63 |
Total HMG dose (IU) | 941.56 ± 879.44 |
No. of total retrieved enbryos | 5.1 ± 3.47 |
No. of ICSI cases | 25.2% (63/250) |
Clinical pregnancy rate | 49.6% (124/250) |
Live birth rate | 35.2% (88/250) |
Primary infertility | 40.4% (101/250) |
Secondary infertility | 59. 61 (149/250) |
Pregnant Group (n = 124) | Non—Pregnant Group (n = 126) | p-Value | Live Birth (n = 87) | Nonlive Birth (n = 163) | p-Value | |
---|---|---|---|---|---|---|
Age of woman (yr) | 32.11 ± 4.39 | 36.44 ± 5.97 | 0 | 31.86 ± 4.26 | 35.59 ± 5.9 | 0 |
LH on hCG day (mIU/mL) | 14.32 ± 15.88 | 23.42 ± 28.51 | 0.002 | 15.42 ± 16.6 | 20.45 ± 26.61 | NS |
Endometrial thickness (mm) | 8.69 ± 1.83 | 8.11 ± 1.23 | 0.003 | 8.84 ± 1.77 | 8.17 ± 1.43 | 0.003 |
BMI (kg/m2) | 22.8 ± 3.1 | 22.81 ± 2.71 | NS | 22.47 ± 2.94 | 22.95 ± 2.85 | NS |
AFC (#) | 15.12 ± 6.48 | 12.54 ± 6.21 | 0.001 | 14.51 ± 6.33 | 13.45 ± 6.55 | NS |
Basal FSH (mIU/mL) | 7.5 ± 2.99 | 8.45 ± 3.4 | NS | 7.69 ± 3.33 | 8.13 ± 3.17 | NS |
Basal LH (mIU/mL) | 5.09 ± 3.09 | 4.64 ± 2.79 | NS | 4.91 ± 2.93 | 4.82 ± 2.96 | NS |
Basal E2 (pg/mL) | 50.26 ± 30.7 | 50.39 ± 34.62 | NS | 50.18 ± 28.79 | 50.58 ± 34.68 | NS |
Duration of infertility(yr) | 3.93 ± 2.65 | 4.8 ± 4.34 | NS | 4.16 ± 2.76 | 4.48 ± 4.0 | NS |
E2 on hCG day (pg/mL) | 690.85 ± 588.45 | 717.31 ± 509.02 | NS | 640.3 ± 441.32 | 737.96 ± 597.87 | NS |
No. of enbryos transferred | 1.85 ± 0.35 | 1.63 ± 0.49 | 0 | 1.86 ± 0.34 | 1.67 ± 0.46 | 0 |
Time of HMG(d) | 9.71 ± 5.28 | 9.06 ± 5.8 | NS | 9.11 ± 4.35 | 9.53 ± 6.13 | NS |
Total HMG dose (IU) | 995.8 ± 887.56 | 877.42 ± 870.12 | NS | 902.96 ± 626.02 | 957.13 ± 950.26 | NS |
No. of total embryos | 6.02 ± 3.67 | 4.19 ± 3.02 | 0 | 6.18 ± 3.91 | 4.51 ± 3.06 | 0.001 |
No. of IVF cases | 75.81% (94/124) | 73.81% (93/126) | NS | 78.16% (68/87) | 73% (119/163) | NS |
Primary infertility | 42.74% (53/124) | 38.1% (48/126) | NS | 45.97 (40/87) | 37.42% (61/163) | NS |
Area under Curve | 95% CI | Cut-Off Value | Sensitivity | Specificity | p-Value | |
---|---|---|---|---|---|---|
LH on hCG day (mIU/mL) | 0.609 | 0.539–0.678 | 8.46 | 51.6 | 67.5 | 0.003 |
Age of woman (yr) | 0.707 | 0.642–0.771 | 37.5 | 88.7 | 46.8 | 0 |
Endometrial thickness (m) | 0.583 | 0.513–0.654 | 7.75 | 71 | 42.9 | 0.023 |
AFC (#) | 0.619 | 0.549–0.689 | 10.5 | 74.2 | 47.6 | 0.001 |
No. of enbryos transferred | 0.614 | 0.544–0.684 | 1.5 | 85.5 | 37.3 | 0.002 |
No. of total enbryos | 0.67 | 0.603–0.737 | 4.5 | 60.5 | 72.7 | 0 |
Area under Curve | 95% CI | Cut-Off Value | Sensitivity | Specificity | p-Value | |
---|---|---|---|---|---|---|
LH on hCG day (mIU/mL) | 0.55 | 0.476–0.625 | 8.46 | 49.4 | 62 | 0.038 |
Age of woman (yr) | 0.679 | 0.514–0.745 | 33.5 | 67.8 | 63.2 | 0 |
Endometrial thickness (m) | 0.623 | 0.549–0.698 | 8.75 | 47.1 | 73 | 0.001 |
AFC (#) | 0.552 | 0.478–0.626 | 10.5 | 71.3 | 41.1 | NS |
No. of enbryos transferred | 0.594 | 0.522–0.665 | 1.5 | 86.2 | 32.5 | 0.015 |
No. of total enbryos | 0.64 | 0.569–0.711 | 4.5 | 60.9 | 65 | 0 |
Clinical Pregnancy | Live Birth | |||||
---|---|---|---|---|---|---|
p | OR | 95% CI | p | OR | 95% CI | |
LH on hCG day (mIU/mL) | 0.02 | 1.02 | 1.0–1.03 | 0.241 | 0.992 | 0.978–1.006 |
Age of woman (yr) | 0 | 1.13 | 1.07–1.2 | 0 | 0.884 | 0.830–0.942 |
Endometrial thickness (mm) | 0.108 | 0.85 | 0.69–1.04 | 0.008 | 1.317 | 1.075–1.612 |
AFC (#) | 0.3 | 1.03 | 0.98–1.09 | 0.005 | 0.92 | 0.867–0.975 |
No. of enbryos transferred | 0.006 | 0.38 | 0.19–0.75 | 0.008 | 2.816 | 1.308–6.061 |
No. of total enbryos | 0.04 | 0.86 | 0.78–0.95 | 0.001 | 1.183 | 1.072–1.306 |
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Shi, Q.; Jiang, Y.; Kong, N.; Huang, C.; Liu, J.; Shen, X.; Sun, Y.; Lu, F.; Mei, J.; Zhou, J. Serum LH Level on the Day of hCG Administration as a Predictor of the Reproductive Outcomes in Ovulation Induction Cycle Frozen–Thawed Embryo Transfer. J. Pers. Med. 2023, 13, 52. https://doi.org/10.3390/jpm13010052
Shi Q, Jiang Y, Kong N, Huang C, Liu J, Shen X, Sun Y, Lu F, Mei J, Zhou J. Serum LH Level on the Day of hCG Administration as a Predictor of the Reproductive Outcomes in Ovulation Induction Cycle Frozen–Thawed Embryo Transfer. Journal of Personalized Medicine. 2023; 13(1):52. https://doi.org/10.3390/jpm13010052
Chicago/Turabian StyleShi, Qingqing, Yue Jiang, Na Kong, Chenyang Huang, Jingyu Liu, Xiaoyue Shen, Yanxin Sun, Feifei Lu, Jie Mei, and Jianjun Zhou. 2023. "Serum LH Level on the Day of hCG Administration as a Predictor of the Reproductive Outcomes in Ovulation Induction Cycle Frozen–Thawed Embryo Transfer" Journal of Personalized Medicine 13, no. 1: 52. https://doi.org/10.3390/jpm13010052
APA StyleShi, Q., Jiang, Y., Kong, N., Huang, C., Liu, J., Shen, X., Sun, Y., Lu, F., Mei, J., & Zhou, J. (2023). Serum LH Level on the Day of hCG Administration as a Predictor of the Reproductive Outcomes in Ovulation Induction Cycle Frozen–Thawed Embryo Transfer. Journal of Personalized Medicine, 13(1), 52. https://doi.org/10.3390/jpm13010052