Decrease of Hyaluronidase Activity and Suppression of Mouse CD4+ T Lymphocyte Activation by Tomato Juice Saponin Esculeoside B, and Its Sapogenol Esculeogenin B
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of EsB and Esg-B
2.2. Hyaluronidase Inhibitory Assay
2.3. Animals and Splenocyte Isolation
2.4. Cytotoxicity Assay
2.5. ConA Proliferation Assay
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Quantitative Polymerase Chain Reaction (q-PCR) Assay
2.8. Statistical Analysis
3. Results
3.1. Hyaluronidase Inhibition by EsB/Esg-B
3.2. Cytotoxicity of EsB/Esg-B in Splenocytes
3.3. Suppression of T Lymphocyte Proliferative Response by EsB/Esg-B
3.4. EsB/Esg-B Modulation of ConA Blast-Induced Cytokine Level
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
Abbreviation
References
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Forward | Reverse | |
---|---|---|
GAPDH | 5′-CCCAGCAAGGACACTGAGCAAG-3′ | 5′-GGTCTGGGATGGAAATTGTGAGGG-3′ |
IL-4 | 5′-GAAGCCCTACAGACGAGCTCA-3′ | 5′-ACAGGAGAAGGGACGCCAT-3′ |
IFN-γ | 5′TCTGGGCTTCTCCTCCTGCGG-3′ | 5′GGCGCTGGACCTGTGGGTTG-3′ |
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Zhou, J.-R.; Kitahara, N.; Nakamura, H.; Ono, T.; Karashima, R.; Fang, J.; Nohara, T.; Yokomizo, K. Decrease of Hyaluronidase Activity and Suppression of Mouse CD4+ T Lymphocyte Activation by Tomato Juice Saponin Esculeoside B, and Its Sapogenol Esculeogenin B. J. Pers. Med. 2022, 12, 579. https://doi.org/10.3390/jpm12040579
Zhou J-R, Kitahara N, Nakamura H, Ono T, Karashima R, Fang J, Nohara T, Yokomizo K. Decrease of Hyaluronidase Activity and Suppression of Mouse CD4+ T Lymphocyte Activation by Tomato Juice Saponin Esculeoside B, and Its Sapogenol Esculeogenin B. Journal of Personalized Medicine. 2022; 12(4):579. https://doi.org/10.3390/jpm12040579
Chicago/Turabian StyleZhou, Jian-Rong, Nozomi Kitahara, Honami Nakamura, Takuya Ono, Ryohei Karashima, Jun Fang, Toshihiro Nohara, and Kazumi Yokomizo. 2022. "Decrease of Hyaluronidase Activity and Suppression of Mouse CD4+ T Lymphocyte Activation by Tomato Juice Saponin Esculeoside B, and Its Sapogenol Esculeogenin B" Journal of Personalized Medicine 12, no. 4: 579. https://doi.org/10.3390/jpm12040579