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Article

Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line

1
Institute of Molecular Bioimaging and Physiology–National Research Council (IBFM-CNR), 90015 Cefalù, Italy
2
Laboratori Nazionali del SUD, Istituto Nazionale di Fisica Nucleare (LNS-INFN), 95100 Catania, Italy
3
Biophysics Department, GSI Helmholtzzentrum für Schwerionenforschung GmbH, 64291 Darmstadt, Germany
4
Trento Institute for Fundamental Physics and Applications (TIFPA), Istituto Nazionale Fisica Nucleare (INFN), 38123 Trento, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors contributed equally to this work.
Academic Editor: Cynthia Aristei
J. Pers. Med. 2021, 11(4), 308; https://doi.org/10.3390/jpm11040308
Received: 17 March 2021 / Revised: 8 April 2021 / Accepted: 12 April 2021 / Published: 16 April 2021
In Glioblastoma Multiforme (GBM), hypoxia is associated with radioresistance and poor prognosis. Since standard GBM treatments are not always effective, new strategies are needed to overcome resistance to therapeutic treatments, including radiotherapy (RT). Our study aims to shed light on the biomarker network involved in a hypoxic (0.2% oxygen) GBM cell line that is radioresistant after proton therapy (PT). For cultivating cells in acute hypoxia, GSI’s hypoxic chambers were used. Cells were irradiated in the middle of a spread-out Bragg peak with increasing PT doses to verify the greater radioresistance in hypoxic conditions. Whole-genome cDNA microarray gene expression analyses were performed for samples treated with 2 and 10 Gy to highlight biological processes activated in GBM following PT in the hypoxic condition. We describe cell survival response and significant deregulated pathways responsible for the cell death/survival balance and gene signatures linked to the PT/hypoxia configurations assayed. Highlighting the molecular pathways involved in GBM resistance following hypoxia and ionizing radiation (IR), this work could suggest new molecular targets, allowing the development of targeted drugs to be suggested in association with PT. View Full-Text
Keywords: transcriptome; hypoxia; glioblastoma; proton therapy; omic science transcriptome; hypoxia; glioblastoma; proton therapy; omic science
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MDPI and ACS Style

Bravatà, V.; Tinganelli, W.; Cammarata, F.P.; Minafra, L.; Calvaruso, M.; Sokol, O.; Petringa, G.; Cirrone, G.A.P.; Scifoni, E.; Forte, G.I.; Russo, G. Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line. J. Pers. Med. 2021, 11, 308. https://doi.org/10.3390/jpm11040308

AMA Style

Bravatà V, Tinganelli W, Cammarata FP, Minafra L, Calvaruso M, Sokol O, Petringa G, Cirrone GAP, Scifoni E, Forte GI, Russo G. Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line. Journal of Personalized Medicine. 2021; 11(4):308. https://doi.org/10.3390/jpm11040308

Chicago/Turabian Style

Bravatà, Valentina, Walter Tinganelli, Francesco P. Cammarata, Luigi Minafra, Marco Calvaruso, Olga Sokol, Giada Petringa, Giuseppe A.P. Cirrone, Emanuele Scifoni, Giusi I. Forte, and Giorgio Russo. 2021. "Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line" Journal of Personalized Medicine 11, no. 4: 308. https://doi.org/10.3390/jpm11040308

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