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Open AccessArticle

A Proteomic Study of Atherosclerotic Plaques in Men with Coronary Atherosclerosis

1
Research Institute of Internal and Preventive Medicine - Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630089 Novosibirsk, Russia
2
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia
3
The Federal State Budgetary Institution “National Medical Research Center named academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation, 630055 Novosibirsk, Russia
*
Author to whom correspondence should be addressed.
Diagnostics 2019, 9(4), 177; https://doi.org/10.3390/diagnostics9040177
Received: 18 October 2019 / Revised: 5 November 2019 / Accepted: 5 November 2019 / Published: 7 November 2019
(This article belongs to the Section Pathology and Molecular Diagnostics)
Background: To study the changes in protein composition of atherosclerotic plaques at different stages of their development in coronary atherosclerosis using proteomics. Methods: The object of research consisted of homogenates of atherosclerotic plaques from coronary arteries at different stages of development, obtained from 15 patients. Plaque proteins were separated by two-dimensional electrophoresis. The resultant protein spots were identified by the matrix-assisted laser desorption ionization method with peptide mass mapping. Results: Groups of differentially expressed proteins, in which the amounts of proteins differed more than twofold (p < 0.05), were identified in pools of homogenates of atherosclerotic plaques at three stages of development. The amounts of the following proteins were increased in stable atherosclerotic plaques at the stage of lipidosis and fibrosis: vimentin, tropomyosin β-chain, actin, keratin, tubulin β-chain, microfibril-associated glycoprotein 4, serum amyloid P-component, and annexin 5. In plaques at the stage of fibrosis and calcification, the amounts of mimecan and fibrinogen were increased. In unstable atherosclerotic plaque of the necrotic–dystrophic type, the amounts of human serum albumin, mimecan, fibrinogen, serum amyloid P-component and annexin were increased. Conclusion: This proteomic study identifies the proteins present in atherosclerotic plaques of coronary arteries by comparing their proteomes at three different stages of plaque development during coronary atherosclerosis. View Full-Text
Keywords: atherosclerosis; proteomics; mass spectrometry; atherosclerotic plaque; tropomyosin atherosclerosis; proteomics; mass spectrometry; atherosclerotic plaque; tropomyosin
MDPI and ACS Style

Stakhneva, E.M.; Meshcheryakova, I.A.; Demidov, E.A.; Starostin, K.V.; Sadovski, E.V.; Peltek, S.E.; Voevoda, M.I.; Chernyavskii, A.M.; Volkov, A.M.; Ragino, Y.I. A Proteomic Study of Atherosclerotic Plaques in Men with Coronary Atherosclerosis. Diagnostics 2019, 9, 177.

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