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Open AccessArticle

Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma

1
Dental Clinical Research Core, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
2
Experimental Medicine Section, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Diagnostics 2019, 9(3), 89; https://doi.org/10.3390/diagnostics9030089
Received: 18 July 2019 / Revised: 29 July 2019 / Accepted: 31 July 2019 / Published: 6 August 2019
(This article belongs to the Section Point-of-Care Diagnostics and Devices)
Point-of-care tests are needed for the screening of head and neck squamous cell carcinoma (HNSCC) and other malignancies. Luciferase immunoprecipitation systems (LIPS), employing light-emitting proteins, were used to examine serum antibodies against several cancer-associated targets in blood donor controls and subjects with colon cancer (CC) and HNSCC. The assessment of antibodies against the wild type p53 tumor antigen showed that approximately 25% of the CC and 20% of the HNSCC patients were seropositive. In addition, humoral responses against two p53 mutants, p53-R175H and p53-R273H, generally tracked the antibody responses seen against wild type p53. Analysis of antibodies against highly specific biomarkers of HPV-16-associated malignancy, E2, E6, and E7 oncoproteins, revealed no seropositivity in blood donors and CC patients. However, 45% (9/20) of the HNSCC patients showed E6 seropositivity, which overlapped all the detectable E2 (40%; 8/20) and E7 seropositive subjects (35%; 7/20). Using neodymium magnets, ultrarapid LIPSTICKS testing of HPV-16 E6 antibodies in <60 s per HNSCC sample demonstrated almost the same diagnostic performance (40% sensitivity and 100% specificity) as LIPS testing in 2.5 h. While additional improvements and standardization are needed, these results highlight the possibility of using these approaches for the diagnosis of HPV-16-associated HNSCC. View Full-Text
Keywords: tumor antigens; head and neck cancer; human papilloma virus-16 (HPV-16); luciferase immunoprecipitation systems (LIPS) tumor antigens; head and neck cancer; human papilloma virus-16 (HPV-16); luciferase immunoprecipitation systems (LIPS)
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Burbelo, P.D.; Chaturvedi, A.; Notkins, A.L.; Gunti, S. Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma. Diagnostics 2019, 9, 89.

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