Next Article in Journal
Automatic Classification of Histopathology Images across Multiple Cancers Based on Heterogeneous Transfer Learning
Next Article in Special Issue
Detection of SARS-CoV-2 Variants via Different Diagnostics Assays Based on Single-Nucleotide Polymorphism Analysis
Previous Article in Journal
The Diagnostic Challenge of Osteoid Osteoma in the Bones of the Hand—A Case Series
Previous Article in Special Issue
Association between Vitamin D Status and Secondary Infections in Patients with Severe COVID-19 Admitted in the Intensive Care Unit of a Tertiary-Level Hospital in Turkey
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Diagnostics
Volume 13
Issue 7
10.3390/diagnostics13071280
diagnostics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Virus-Specific Stem Cell Memory CD8+ T Cells May Indicate a Long-Term Protection against Evolving SARS-CoV-2

by
Milena Aleksova
1,*,
Yana Todorova
1,
Radoslava Emilova
1,
Magdalena Baymakova
2,
Nina Yancheva
3,
Radina Andonova
2,
Anelia Zasheva
2,
Alba Grifoni
4,
Daniela Weiskopf
4,
Alessandro Sette
4,5 and
Maria Nikolova
1
1
Immunology Department, National Center of Infectious and Parasitic Diseases, 1000 Sofia, Bulgaria
2
Department of Infectious Diseases, Military Medical Academy, 1000 Sofia, Bulgaria
3
Specialized Hospital for Active Treatment of Infectious and Parasitic Diseases, 1000 Sofia, Bulgaria
4
Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), Conl Public Health, University of California San Diego (UCSD), San Diego, CA 92037, USA
5
Department of Pathology, University of California La Jolla, San Diego, CA 92093, USA
*
Author to whom correspondence should be addressed.
Diagnostics 2023, 13(7), 1280; https://doi.org/10.3390/diagnostics13071280
Submission received: 16 December 2022 / Revised: 15 February 2023 / Accepted: 20 March 2023 / Published: 28 March 2023
(This article belongs to the Special Issue Detection and Assessment of SARS-CoV-2 Variants)
Browse Figures
Versions Notes

Abstract

Immune memory to SARS-CoV-2 is key for establishing herd immunity and limiting the spread of the virus. The duration and qualities of T-cell-mediated protection in the settings of constantly evolving pathogens remain an open question. We conducted a cross-sectional study of SARS-CoV-2-specific CD4+ and CD8+ T-cell responses at several time points over 18 months (30–750 days) post mild/moderate infection with the aim to identify suitable methods and biomarkers for evaluation of long-term T-cell memory in peripheral blood. Included were 107 samples from 95 donors infected during the periods 03/2020–07/2021 and 09/2021–03/2022, coinciding with the prevalence of B.1.1.7 (alpha) and B.1.617.2 (delta) variants in Bulgaria. SARS-CoV-2-specific IFNγ+ T cells were measured in ELISpot in parallel with flow cytometry detection of AIM+ total and stem cell-like memory (TSCM) CD4+ and CD8+ T cells after in vitro stimulation with peptide pools corresponding to the original and delta variants. We show that, unlike IFNγ+ T cells, AIM+ virus-specific CD4+ and CD8+ TSCM are more adequate markers of T cell memory, even beyond 18 months post-infection. In the settings of circulating and evolving viruses, CD8+ TSCM is remarkably stable, back-differentiated into effectors, and delivers immediate protection, regardless of the initial priming strain.
Keywords: SARS-CoV-2; B.1.1.7 (alpha) variant; B.1.617.2 (delta) variant; biomarkers; stem cell-like memory T cells (TSCM) SARS-CoV-2; B.1.1.7 (alpha) variant; B.1.617.2 (delta) variant; biomarkers; stem cell-like memory T cells (TSCM)

Share and Cite

MDPI and ACS Style

Aleksova, M.; Todorova, Y.; Emilova, R.; Baymakova, M.; Yancheva, N.; Andonova, R.; Zasheva, A.; Grifoni, A.; Weiskopf, D.; Sette, A.; et al. Virus-Specific Stem Cell Memory CD8+ T Cells May Indicate a Long-Term Protection against Evolving SARS-CoV-2. Diagnostics 2023, 13, 1280. https://doi.org/10.3390/diagnostics13071280

AMA Style

Aleksova M, Todorova Y, Emilova R, Baymakova M, Yancheva N, Andonova R, Zasheva A, Grifoni A, Weiskopf D, Sette A, et al. Virus-Specific Stem Cell Memory CD8+ T Cells May Indicate a Long-Term Protection against Evolving SARS-CoV-2. Diagnostics. 2023; 13(7):1280. https://doi.org/10.3390/diagnostics13071280

Chicago/Turabian Style

Aleksova, Milena, Yana Todorova, Radoslava Emilova, Magdalena Baymakova, Nina Yancheva, Radina Andonova, Anelia Zasheva, Alba Grifoni, Daniela Weiskopf, Alessandro Sette, and et al. 2023. "Virus-Specific Stem Cell Memory CD8+ T Cells May Indicate a Long-Term Protection against Evolving SARS-CoV-2" Diagnostics 13, no. 7: 1280. https://doi.org/10.3390/diagnostics13071280

APA Style

Aleksova, M., Todorova, Y., Emilova, R., Baymakova, M., Yancheva, N., Andonova, R., Zasheva, A., Grifoni, A., Weiskopf, D., Sette, A., & Nikolova, M. (2023). Virus-Specific Stem Cell Memory CD8+ T Cells May Indicate a Long-Term Protection against Evolving SARS-CoV-2. Diagnostics, 13(7), 1280. https://doi.org/10.3390/diagnostics13071280

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop