Polyamine Metabolites as Biomarkers in Head and Neck Cancer Biofluids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Biofluid Samples
2.3. Sample Processing
2.4. LC-MS/MS Analysis
2.5. Data Processing
2.6. Statistics
3. Results
3.1. HNC Population Characteristics
3.2. Polyamine Levels in HNC-Derived Saliva
3.3. Polyamine Levels in HNC-Derived Urine
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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HNC Cohort | Characteristic | Number (Percentage) |
---|---|---|
Age | 66 (range: 39–83) | |
Gender | Male | 23 (59) |
Female | 16 (41) | |
Site | Oral Cavity | 21 (54) |
Oropharynx | 16 (41) | |
Larynx | 1 (3) | |
Nasopharynx | 1 (3) | |
Stage | I | 11(28) |
II | 8 (21) | |
III | 5 (13) | |
IV | 15 (38) | |
P16 Status | Positive | 9 (23) |
Negative | 30 (77) |
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DeFelice, B.C.; Fiehn, O.; Belafsky, P.; Ditterich, C.; Moore, M.; Abouyared, M.; Beliveau, A.M.; Farwell, D.G.; Bewley, A.F.; Clayton, S.M.; et al. Polyamine Metabolites as Biomarkers in Head and Neck Cancer Biofluids. Diagnostics 2022, 12, 797. https://doi.org/10.3390/diagnostics12040797
DeFelice BC, Fiehn O, Belafsky P, Ditterich C, Moore M, Abouyared M, Beliveau AM, Farwell DG, Bewley AF, Clayton SM, et al. Polyamine Metabolites as Biomarkers in Head and Neck Cancer Biofluids. Diagnostics. 2022; 12(4):797. https://doi.org/10.3390/diagnostics12040797
Chicago/Turabian StyleDeFelice, Brian C., Oliver Fiehn, Peter Belafsky, Constanze Ditterich, Michael Moore, Marianne Abouyared, Angela M. Beliveau, D. Gregory Farwell, Arnaud F. Bewley, Shannon M. Clayton, and et al. 2022. "Polyamine Metabolites as Biomarkers in Head and Neck Cancer Biofluids" Diagnostics 12, no. 4: 797. https://doi.org/10.3390/diagnostics12040797