Urinary Protein Profiling for Potential Biomarkers of Chronic Kidney Disease: A Pilot Study
, Zhalaliddin Makhammajanov 3,†, Zhanna Dauyey 1, Zhannur Markhametova 1, Kamilla Mussina 1, Assem Nogaibayeva 4, Larissa Kozina 5, Dana Auganova 6, Pavel Tarlykov 6, Rostislav Bukasov 7, Zhandos Utegulov 8, Duman Turebekov 9, Maria Jose Soler 10,11, Alberto Ortiz 12 and Mehmet Kanbay 13
Rajasree Menon
Round 1
Reviewer 1 Report
As the authors suggested a thorough understanding of underlying processes in CKD will provide an effective strategy for preventing CKD-associated social economic burdens and mortalities. Even though the sample size and the total protein yield is moderate, this study could be a useful resource for kidney researchers. Especially when proteomic findings provide as validation for high throughout transcriptomic findings. The language is easy to understand. One mistake observed was: It seems like the authors made a mistake while stating the age bracket for the exclusion group in selecting the patients for the study.
Author Response
Hello
Point 1: As the authors suggested a thorough understanding of underlying processes in CKD will provide an effective strategy for preventing CKD-associated social economic burdens and mortalities. Even though the sample size and the total protein yield is moderate, this study could be a useful resource for kidney researchers. Especially when proteomic findings provide as validation for high throughout transcriptomic findings. The language is easy to understand.
Response 1: We appreciate the comment of the reviewer.
Point 2: One mistake observed was: It seems like the authors made a mistake while stating the age bracket for the exclusion group in selecting the patients for the study.
Response 2: Thank you for the comment. This mistake has been corrected.
. Please see the attachment.
Reviewer 2 Report
This paper is very valuable for looking for an early biomarker for chronic kidney disease (CKD) as well as a key risk factor for CKD progression. However, further prospective research is recommended with a larger study population to validate the study findings. And it is meaningful to further analysis the relationship between the urinary proteins which were correlated with 24h proteinuria and/or kidney function levels and the primary diseases of CKD.
Author Response
Authors thank the Reviewer for the comment and recommendations. Further correlation analyses between (positively/negatively correlated) urinary proteins and the primary disease of CKD will be considered in future studies.
