Next Article in Journal
The Clinical Importance of IL-6, IL-8, and TNF-α in Patients with Ovarian Carcinoma and Benign Cystic Lesions
Next Article in Special Issue
Discovering the Molecular Landscape of Meningioma: The Struggle to Find New Therapeutic Targets
Previous Article in Journal
Differences between Acute Exacerbations of Idiopathic Pulmonary Fibrosis and Other Interstitial Lung Diseases
Article

Human TERT Promoter Mutations in Atypical and Anaplastic Meningiomas

1
Dipartimento di Scienze della Salute, Scuola di Medicina, Università del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy
2
Centro Interdipartimentale di Ricerca Traslazionale Sulle Malattie Autoimmuni & Allergiche (CAAD), Università del Piemonte Orientale (UPO), Corso Trieste 15A, 28100 Novara, Italy
3
Unità di Radioterapia, Dipartimento di Medicina Traslazionale, Università del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy
4
Unità di Radioterapia Oncologica, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 18, 28100 Novara, Italy
5
Unità di Patologia, Azienda Ospedaliera Universitaria Maggiore della Carità, Corso Mazzini 18, 28100 Novara, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Georgina Tzanakaki
Diagnostics 2021, 11(9), 1624; https://doi.org/10.3390/diagnostics11091624
Received: 20 July 2021 / Revised: 31 August 2021 / Accepted: 3 September 2021 / Published: 6 September 2021
(This article belongs to the Special Issue Molecular Diagnostics of Brain Tumours)
Background: The role of telomerase reverse transcriptase (TERT) gene promoter mutations (pTERT) in atypical and anaplastic meningiomas remains controversial. This study aimed to evaluate their impact on the histologic diagnosis and prognosis in a retrospective series of 74 patients with atypical and anaplastic meningioma, including disease progression and relapse. A supplementary panel of 21 benign tumours was used as a control cohort. Materials and Methods: The mutation rate of the pTERT gene was assessed by Sanger sequencing. ATRX protein expression was detected by immunohistochemistry. The phenotypic and genotypic intra-tumour heterogeneity was studied in a sub-group of 12 cases using a Molecular Machines & Industries (MMI) CellCut laser microdissection (LMD) system. Results: pTERT mutations were detected in 12/74 (17.6%) malignant meningiomas. The mutation rate was significantly higher in anaplastic meningiomas (7/23, 30.4%) compared to atypical tumours (5/48, 10.4%) (p = 0.0443). In contrast, the mutation rate was < 5% in benign tumours. All pTERT mutant cases retained nuclear ATRX immunoreactivity. pTERT mutations were significantly associated with the histologic grade (p = 0.0443) and were adverse prognostic factors for anaplastic tumours (p = 0.06). Conclusion: We reported on the pTERT mutation spectrum in malignant meningiomas, supporting their use in the prognostic classification. View Full-Text
Keywords: meningiomas; telomerase reverse transcriptase; molecular markers; ALT; prognosis meningiomas; telomerase reverse transcriptase; molecular markers; ALT; prognosis
Show Figures

Graphical abstract

MDPI and ACS Style

Mellai, M.; Porrini Prandini, O.; Mustaccia, A.; Fogazzi, V.; Allesina, M.; Krengli, M.; Boldorini, R. Human TERT Promoter Mutations in Atypical and Anaplastic Meningiomas. Diagnostics 2021, 11, 1624. https://doi.org/10.3390/diagnostics11091624

AMA Style

Mellai M, Porrini Prandini O, Mustaccia A, Fogazzi V, Allesina M, Krengli M, Boldorini R. Human TERT Promoter Mutations in Atypical and Anaplastic Meningiomas. Diagnostics. 2021; 11(9):1624. https://doi.org/10.3390/diagnostics11091624

Chicago/Turabian Style

Mellai, Marta, Omar Porrini Prandini, Aurora Mustaccia, Valentina Fogazzi, Marta Allesina, Marco Krengli, and Renzo Boldorini. 2021. "Human TERT Promoter Mutations in Atypical and Anaplastic Meningiomas" Diagnostics 11, no. 9: 1624. https://doi.org/10.3390/diagnostics11091624

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop