Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Bone Marrow Transplantation
2.3. In Vivo Confocal Microscopy
2.4. Fluorescein Staining on the Ocular Surface
2.5. Cotton Thread Test
2.6. Cell Count in the Cornea
2.7. Histological Analysis
2.8. cGVHD Patients
2.9. Statistical Analysis
3. Results
3.1. The Observation of Mouse Cornea by IVCM
3.1.1. Serial Changes of Corneal Findings Observed by In Vivo Confocal Microscopy
3.1.2. Neovascularization
3.1.3. Characteristic Cell Types in the Cornea
3.1.4. The Change of Nerve Fibers
3.2. Serial Changes of Corneal Fluorescein Staining and Tear Fluid Volume
3.3. Histopathological Findings of Cornea and Lacrimal Gland in Syngeneic and Allogeneic Mice
3.4. cGVHD Patients
4. Discussion
5. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
GVHD | Graft-versus-host disease |
HSCT | Hematopoietic stem cell transplantation |
BMT | Bone marrow transplantation |
NIH | National Institute of Health |
IVCM | In vivo confocal microscopy |
DED | Dry eye disease |
RPMI | Roswell Park Memorial Institute |
ACK | ammonium-chloride-potassium |
HRT2-RCM | Heidelberg Retina Tomograph 2 Rostock Cornea Module |
PMMA | poly-methyl-methacrylate |
CFS | Corneal fluorescein staining |
HE | Hematoxylin & Eosin |
NGF | Nerve growth factor |
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Shimizu, S.; Sato, S.; Taniguchi, H.; Shimizu, E.; He, J.; Hayashi, S.; Negishi, K.; Ogawa, Y.; Shimmura, S. Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy. Diagnostics 2021, 11, 1515. https://doi.org/10.3390/diagnostics11081515
Shimizu S, Sato S, Taniguchi H, Shimizu E, He J, Hayashi S, Negishi K, Ogawa Y, Shimmura S. Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy. Diagnostics. 2021; 11(8):1515. https://doi.org/10.3390/diagnostics11081515
Chicago/Turabian StyleShimizu, Shota, Shinri Sato, Hiroko Taniguchi, Eisuke Shimizu, Jingliang He, Shunsuke Hayashi, Kazuno Negishi, Yoko Ogawa, and Shigeto Shimmura. 2021. "Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy" Diagnostics 11, no. 8: 1515. https://doi.org/10.3390/diagnostics11081515