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Article

Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population

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Department of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
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Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
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Clinical Department for Lung Diseases Jordanovac, University Hospital Centre Zagreb, 10000 Zagreb, Croatia
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School of Medicine, University of Zagreb, 10000 Zagreb, Croatia
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Clinical Institute of Laboratory Diagnostics, University Hospital Centre Zagreb, 10000 Zagreb, Croatia
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Ruđer Bošković Institute, Division of Molecular Medicine, Laboratory for Advanced Genomics, 10000 Zagreb, Croatia
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Department of Integrative Medicine, Faculty for Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia
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German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Division of Pediatric Neuroon-Cology, 69120 Heidelberg, Germany
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Hopp Children’s Cancer Center (KiTZ), 69120 Heidelberg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Fabiano Di Marco
Diagnostics 2021, 11(8), 1412; https://doi.org/10.3390/diagnostics11081412
Received: 8 July 2021 / Revised: 31 July 2021 / Accepted: 2 August 2021 / Published: 4 August 2021
(This article belongs to the Section Pathology and Molecular Diagnostics)
Heat shock protein 70 (Hsp70) engages Toll-like receptors (TLR) 2 and 4 when found in the extracellular compartment and contributes to inflammation in chronic obstructive pulmonary disease (COPD). Since there is growing evidence for the genetic risk factors for COPD, the gene expression of HSP70, TLR2 and TLR4 was determined, as well as the association between HSP70, TLR2 and TLR4 single nucleotide polymorphisms, (SNPs) and COPD. The gene expression was assessed in peripheral blood cells of 137 COPD patients and 95 controls by a quantitative polymerase chain reaction (qPCR), while a total of nine SNPs were genotyped by TaqMan allelic discrimination real-time PCR. HSP70 and TLR2 gene expression was increased in COPD patients compared to the controls, regardless of the disease severity and smoking status of participants. The rs6457452 SNP of HSP70 was associated with COPD, indicating the protective role of the T allele (OR = 0.46, 95% CI = 0.24–0.89, p = 0.022). Furthermore, COPD C/T heterozygotes showed a decreased HSP70 mRNA level compared to COPD C/C homozygotes. In conclusion, HSP70 and TLR2 may have a role in the pathogenesis of COPD, and the HSP70 rs6457452 variant might influence the genetic susceptibility to COPD in the Croatian population. View Full-Text
Keywords: chronic obstructive pulmonary disease; heat shock protein 70; toll-like receptor 2; toll-like receptor 4; single nucleotide polymorphism chronic obstructive pulmonary disease; heat shock protein 70; toll-like receptor 2; toll-like receptor 4; single nucleotide polymorphism
MDPI and ACS Style

Hlapčić, I.; Grdić Rajković, M.; Čeri, A.; Dabelić, S.; Popović-Grle, S.; Radić Antolic, M.; Knežević, J.; Försti, A.; Rumora, L. Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population. Diagnostics 2021, 11, 1412. https://doi.org/10.3390/diagnostics11081412

AMA Style

Hlapčić I, Grdić Rajković M, Čeri A, Dabelić S, Popović-Grle S, Radić Antolic M, Knežević J, Försti A, Rumora L. Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population. Diagnostics. 2021; 11(8):1412. https://doi.org/10.3390/diagnostics11081412

Chicago/Turabian Style

Hlapčić, Iva, Marija Grdić Rajković, Andrea Čeri, Sanja Dabelić, Sanja Popović-Grle, Margareta Radić Antolic, Jelena Knežević, Asta Försti, and Lada Rumora. 2021. "Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population" Diagnostics 11, no. 8: 1412. https://doi.org/10.3390/diagnostics11081412

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