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Article

Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples

1
The Pirbright Institute, Woking, Surrey GU24 0NF, UK
2
VetWork Diagnostix, 820166 Tulcea, Romania
3
Emergency Hospital Bucharest, Molecular Pathology Laboratory, 050098 Bucharest, Romania
4
Personal Genetics, 010987 Bucharest, Romania
5
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(7), 472; https://doi.org/10.3390/diagnostics10070472
Received: 27 June 2020 / Revised: 8 July 2020 / Accepted: 9 July 2020 / Published: 11 July 2020
Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their sensitivity and feasibility for use in pooling. In this preliminary study, we showed that pooling of up to 80 samples did not affect the efficacy of the kits. Additionally, the RNA-dependent RNA polymerase (RdRp) gene is a more suitable target in pooled samples than the envelope (E) gene. This approach could provide an easy method of screening a large number of samples and help adjust different governmental regulations. View Full-Text
Keywords: SARS-CoV-2; PCR; diagnostics; pooling strategy SARS-CoV-2; PCR; diagnostics; pooling strategy
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MDPI and ACS Style

Petrovan, V.; Vrajmasu, V.; Bucur, A.C.; Soare, D.S.; Radu, E.; Dimon, P.; Zaulet, M. Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples. Diagnostics 2020, 10, 472. https://doi.org/10.3390/diagnostics10070472

AMA Style

Petrovan V, Vrajmasu V, Bucur AC, Soare DS, Radu E, Dimon P, Zaulet M. Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples. Diagnostics. 2020; 10(7):472. https://doi.org/10.3390/diagnostics10070472

Chicago/Turabian Style

Petrovan, Vlad, Virgil Vrajmasu, Ana C. Bucur, Dan S. Soare, Eugen Radu, Paula Dimon, and Mihaela Zaulet. 2020. "Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples" Diagnostics 10, no. 7: 472. https://doi.org/10.3390/diagnostics10070472

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