Next Article in Journal
The Value of 68Ga-PSMA PET/CT Following Equivocal 18F-NaF PET/CT in Prostate Cancer Patients
Previous Article in Journal
Intraoperative Sphenoid Sinus Volume Measurement as an Alternative Technique to Intraoperative Computer Tomography
Open AccessArticle

Epithelial-Mesenchymal Transition Proteins in Neuroendocrine Neoplasms: Differential Immunohistochemical Expression in Different Sites and Correlation with Clinico-Pathological Features

1
Department of Advanced Biomedical Sciences, Pathology Section, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy
2
Pathology Department, Cardarelli Hospital, Via Antonio Cardarelli 9, 80131 Napoli, Italy
3
Pathology Department, Monaldi Hospital, Via Leonardo Bianchi, 80131 Naples, Italy
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(6), 351; https://doi.org/10.3390/diagnostics10060351
Received: 22 April 2020 / Revised: 21 May 2020 / Accepted: 25 May 2020 / Published: 28 May 2020
(This article belongs to the Section Pathology and Molecular Diagnostics)
The first step leading to metastasis, or for the acquisition of local invasiveness, involves changes in the phenotype of neoplastic cells in the primary tumor. The epithelial–mesenchymal transition (EMT) is a process that determines the acquisition of a form and a transcriptional program that are characteristic of mesenchymal cells, in epithelial cells. The factors involved in this process are E-cadherin and N-cadherin adhesion proteins and some transcription factors such as Slug and Twist. EMT is a site-specific mechanism that is also active in embryogenesis—embryonic cells are affected if invested in certain points, probably due to the signals emanating from the cells or groups of surrounding cells. It is known that neuroendocrine neoplasms have a biological behavior that differs in grading, staging, and site. The aim of our study was to investigate the immunohistochemical expression of EMT factors (Twist, Slug, and E-cadherin) in the neuroendocrine neoplasms of the gastrointestinal tract, the pancreas, and lungs, in 65 cases retrieved from the archives of the Department of Pathology, of three hospitals. The immunoscores were compared in each site and correlated with the clinico-pathological parameters. Statistical evaluation revealed an association between the higher Twist immunoscore and higher grading (p value < 0.0001) and staging (p value = 0.0055). Slug was detected only in pancreatic cases where its reduced expression was associated with a higher grading (p value = 0.0033). This data could be of diagnostic utility in the case of metastases from neuroendocrine neoplasm, to define the site of the primitive tumor when the traditional immunohistochemical panel is not sufficient. In summary, our results indicated, first that the EMT is also an active process in neuroendocrine neoplasms. To the best of our knowledge, this was the first study that evaluated the expression of EMT factors in neuroendocrine neoplasms of different districts. View Full-Text
Keywords: EMT; slug; twist; Ki67; neuroendocrine EMT; slug; twist; Ki67; neuroendocrine
Show Figures

Figure 1

MDPI and ACS Style

Guadagno, E.; Campione, S.; Pignatiello, S.; Borrelli, G.; De Dominicis, G.; De Rosa, N.; Del Basso De Caro, M. Epithelial-Mesenchymal Transition Proteins in Neuroendocrine Neoplasms: Differential Immunohistochemical Expression in Different Sites and Correlation with Clinico-Pathological Features. Diagnostics 2020, 10, 351.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop