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Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours

1
Research Laboratory, Hospital General Universitario de Alicante-Institute for Health and Biomedical Research (ISABIAL), 03010 Alicante, Spain
2
Department of Endocrinology & Nutrition, Hospital General Universitario de Alicante -ISABIAL, 03010 Alicante, Spain
3
Department of Endocrinology and Nutrition, Hospital La Ribera, Alzira, 46600 Valencia, Spain
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Department of Endocrinology & Nutrition, Hospital Universitario y Politécnico La Fe, 46026 Valencia, Spain
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Department of Endocrinology & Nutrition, Hospital General Universitario de Albacete, 02006 Albacete, Spain
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Department of Pathology, Hospital General Universitario de Alicante -ISABIAL, 03010 Alicante, Spain
7
Department of Endocrinology & Nutrition, Hospital General Universitario de Alicante, Miguel Hernández University, 03010 Alicante, Spain
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(4), 227; https://doi.org/10.3390/diagnostics10040227
Received: 16 March 2020 / Revised: 12 April 2020 / Accepted: 15 April 2020 / Published: 16 April 2020
(This article belongs to the Section Pathology and Molecular Diagnostics)
miR-17-5p and E2F1 have been described as deregulated in cancer, but they have scarcely been studied in pituitary neuroendocrine tumours (PitNETs). This study evaluates the relationship of E2F1 and miR-17-5p with the invasiveness and proliferation of PitNETs. In this cross-sectional descriptive study, we evaluated the expression of E2F1, MYC, and miR-17-5p by quantitative real time PCR analysis in 60 PitNETs: 29 gonadotroph (GT), 15 functioning somatotroph (ST), and 16 corticotroph (CT) tumours, of which 8 were silent (sCT). The clinical data were collected from the Spanish Molecular Register of Pituitary Adenomas (REMAH) database. We defined invasiveness according to the Knosp classification and proliferation according to a molecular expression of Ki-67 ≥ 2.59. E2F1 was more expressed in invasive than in non-invasive tumours in the whole series (p = 0.004) and in STs (p = 0.01). In addition, it was overexpressed in the silent subtypes (GTs and sCTs; all macroadenomas) and normoexpressed in the functioning ones (fCTs and STs; some microadenomas). miR-17-5p was more expressed in proliferative than in non-proliferative tumours (p = 0.041) in the whole series but not by subtypes. Conclusions: Our study suggests that in PitNETs, E2F1 could be a good biomarker of invasiveness, and miR-17-5p of proliferation, helping the clinical management of these tumours. View Full-Text
Keywords: miR-17~92; pituitary neuroendocrine tumours; E2F1; microRNAs miR-17~92; pituitary neuroendocrine tumours; E2F1; microRNAs
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García-Martínez, A.; López-Muñoz, B.; Fajardo, C.; Cámara, R.; Lamas, C.; Silva-Ortega, S.; Aranda, I.; Picó, A. Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours. Diagnostics 2020, 10, 227.

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