Next Article in Journal
Dysgerminoma with a Somatic Exon 17 KIT Mutation and SHH Pathway Activation in a Girl with Turner Syndrome
Next Article in Special Issue
MicroRNAs: An Update of Applications in Forensic Science
Previous Article in Journal
Extracellular Vesicles in Hematological Malignancies: From Biomarkers to Therapeutic Tools
Previous Article in Special Issue
Postmortem Protein Degradation as a Tool to Estimate the PMI: A Systematic Review
Article

Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study

1
Department of Forensic Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan
2
Department of Forensic Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
3
Second Biology Section, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan
4
Department of Advanced Biomaterials, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(12), 1066; https://doi.org/10.3390/diagnostics10121066
Received: 13 November 2020 / Revised: 4 December 2020 / Accepted: 7 December 2020 / Published: 9 December 2020
(This article belongs to the Special Issue Progress in the Forensic Diagnosis)
The postmortem interval (PMI) of victims is a key parameter in criminal investigations. However, effective methods for estimating the PMI of skeletal remains have not been established because it is determined by various factors, including environmental conditions. To identify effective parameters for estimating the PMI of skeletal remains, we investigated the change in bone focusing on the amount of DNA, element concentrations, and bone density that occurred in the bone samples of bovine femurs, each maintained under one of five simulated environmental conditions (seawater, freshwater, underground, outdoors, and indoors) for 1 year. The amount of extracted mitochondrial DNA (mtDNA; 404 bp fragment) decreased over time, and significant DNA degradation (p < 0.01), as estimated by a comparison with amplification results for a shorter fragment (128 bp), was detected between 1 month and 3 months. Eleven of 30 elements were detected in samples by inductively coupled plasma optical emission spectrometry, and Na and Ba showed significant quantitative differences in terms of environmental conditions and time (p < 0.01). This preliminary study suggests that the level of DNA degradation determined by real-time polymerase chain reaction and element concentrations determined by inductively coupled plasma optical emission may be useful indices for estimating the PMI of victims under a wide range of environmental conditions. However, this study is a limited experimental research and not applicable to forensic cases as it is. Further studies of human bone with longer observation periods are required to verify these findings and to establish effective methods for PMI estimation. View Full-Text
Keywords: bone density; DNA degradation; element concentration; postmortem interval; skeletal remains; inductively coupled plasma optical emission spectrometry bone density; DNA degradation; element concentration; postmortem interval; skeletal remains; inductively coupled plasma optical emission spectrometry
Show Figures

Figure 1

MDPI and ACS Style

Nagai, M.; Sakurada, K.; Imaizumi, K.; Ogawa, Y.; Uo, M.; Funakoshi, T.; Uemura, K. Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study. Diagnostics 2020, 10, 1066. https://doi.org/10.3390/diagnostics10121066

AMA Style

Nagai M, Sakurada K, Imaizumi K, Ogawa Y, Uo M, Funakoshi T, Uemura K. Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study. Diagnostics. 2020; 10(12):1066. https://doi.org/10.3390/diagnostics10121066

Chicago/Turabian Style

Nagai, Midori, Koichi Sakurada, Kazuhiko Imaizumi, Yoshinori Ogawa, Motohiro Uo, Takeshi Funakoshi, and Koichi Uemura. 2020. "Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study" Diagnostics 10, no. 12: 1066. https://doi.org/10.3390/diagnostics10121066

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop