The Significance of Fibrosis Quantification as a Marker in Assessing Pseudo-Capsule Status and Clear Cell Renal Cell Carcinoma Prognosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Tumor Sample Selection
2.2. SHG and TPEF Imaging
2.3. Quantification of Collagen Morphology Parameters
2.4. Statistical Analysis
3. Results
3.1. Association between ITF and Clinicopathological Characteristics
3.2. Association between PC Fibrosis and Clinicopathological Characteristics
3.3. PC fibrosis was a Marker for PC Invasion
3.4. The Prognostic Role of PC CPA as a Marker
4. Discussion
5. Conclusion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations:
ccRCC | clear cell renal cell carcinoma |
SHG/TPEF | second harmonic generation/two-photon excitation fluorescence |
PC | Pseudo-capsule |
CPA | collagen proportional area |
AggCPA | percentage of aggregated collagen proportional area |
DisCPA | percentage of distributed collagen proportional area |
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Features | Number, n (n = 73) | |
---|---|---|
Gender | Male | 54 |
Female | 19 | |
Age(years) | ≤58 | 39 |
>58 | 34 | |
Fuhrman grade | G1+2 | 54 |
G3+4 | 19 | |
Clinical stage | I+II | 47 |
III+IV | 26 | |
Tumor size | ≤4 cm | 47 |
>4 cm | 26 | |
Intra-tumoral necrosis | Absent | 54 |
Present | 19 | |
PC status | PS- | 23 |
PS+ | 50 |
Factors | Univariate Analysis | Multivariate Analysis | |||
---|---|---|---|---|---|
OR(95% CI) | p Value | OR(95% CI) | p Value | ||
Gender | male vs. female | 0.995 (0.323–3.066) | 0.994 | ||
Age (year) | ≤58 vs. >58 | 1.200 (0.444–3.241) | 0.719 | ||
Nuclear Grade | G1+2 vs. G3+4 | 1.400 (0.436–4.499) | 0.572 | 1.019 (0.274–3.782) | 0.978 |
Clinical Stage | I + II vsIII + IV | 3.732 (1.108–12.569) | 0.034 | 2.128 (0.563–8.043) | 0.265 |
Tumor Size | ≤4 cm vs. >4 cm | 5.679 (1.495–21.577) | 0.011 | 6.409 (1.453–28.264) | 0.014 |
Intra-tumoral necrosis | absent vs. present | 1.400 (0.436–4.499) | 0.572 | ||
Tumor side | left vs. right | 0.562 (0.206–1.537) | 0.261 | ||
PC | CPA | 0.925 (0.863–0.992) | 0.028 | 0.923 (0.854–0.998) | 0.044 |
AggCPA | 0.925 (0.864–0.991) | 0.026 | |||
DisCPA | 1.116 (0.620–2.008) | 0.714 | |||
Tumor | CPA | 0.942 (0.845–1.051) | 0.285 | ||
AggCPA | 0.942 (0.840–1.057) | 0.312 | |||
DisCPA | 0.399 (0.078–2.026) | 0.268 |
Factors | Univariate Analysis | Multivariate Analysis | |||
---|---|---|---|---|---|
HR (95% CI) | p Value | HR (95% CI) | p Value | ||
Gender | male vs. female | 0.493 (0.169–1.435) | 0.194 | ||
Age (year) | ≤58 vs. >58 | 0.943 (0.428–2.078) | 0.884 | ||
Fuhrman Grade | G1+2 vs. G2+4 | 1.532 (0.660–3.588) | 0.321 | 1.259 (0.526–3.013) | 0.605 |
Clinical Stage | I+II vs III + IV | 2.036 (0.926–4.475) | 0.077 | 0.823 (0.324–2.093) | 0.682 |
Tumor Size | ≤4 cm vs. >4 cm | 2.562 (1.159–5.662) | 0.020 | 1.462 (0.608–3.512) | 0.396 |
Tumor side | left vs. right | 0.634 (0.273–1.470) | 0.288 | ||
Histologic Necrosis | Absent vs. present | 2.781 (1.259–6.142) | 0.011 | 2.858 (1.084–7.533) | 0.034 |
PS status | PS- vs. PS+ | 12.957 (1.751–95.869) | 0.012 | 10.529 (1.362–81.419) | 0.024 |
CPA | 0.938 (0.883–0.996) | 0.037 | 0.949 (0.889–1.013) | 0.116 | |
PS | AggCPA | 0.928 (0.870–0.991) | 0.025 | ||
DisCPA | 1.449 (0.948–2.213) | 0.087 | |||
CPA | 1.025 (0.939–1.120) | 0.577 | |||
Tumor | AggCPA | 1.027 (0.935–1.127) | 0.578 | ||
DisCPA | 1.470 (0.411–5.258) | 0.554 |
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Qin, C.; Yin, H.; Liu, H.; Liu, F.; Du, Y.; Xu, T. The Significance of Fibrosis Quantification as a Marker in Assessing Pseudo-Capsule Status and Clear Cell Renal Cell Carcinoma Prognosis. Diagnostics 2020, 10, 895. https://doi.org/10.3390/diagnostics10110895
Qin C, Yin H, Liu H, Liu F, Du Y, Xu T. The Significance of Fibrosis Quantification as a Marker in Assessing Pseudo-Capsule Status and Clear Cell Renal Cell Carcinoma Prognosis. Diagnostics. 2020; 10(11):895. https://doi.org/10.3390/diagnostics10110895
Chicago/Turabian StyleQin, Caipeng, Huaqi Yin, Huixin Liu, Feng Liu, Yiqing Du, and Tao Xu. 2020. "The Significance of Fibrosis Quantification as a Marker in Assessing Pseudo-Capsule Status and Clear Cell Renal Cell Carcinoma Prognosis" Diagnostics 10, no. 11: 895. https://doi.org/10.3390/diagnostics10110895