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Article

Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

1
Environmental Health Institute, National Environment Agency, Singapore 38667, Singapore
2
Bioinformatics Institute, Agency for Science, Technology and Research, Singapore 138671, Singapore
3
Department of Biological Sciences, National University of Singapore, Singapore 117558, Singapore
4
School of Biological Sciences, Nanyang Technological University, Singapore 639798, Singapore
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(1), 11; https://doi.org/10.3390/diagnostics10010011
Received: 7 November 2019 / Revised: 13 December 2019 / Accepted: 23 December 2019 / Published: 24 December 2019
(This article belongs to the Section Diagnostic Microbiology and Infectious Disease)
Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate. View Full-Text
Keywords: flavivirus; diagnostics; nonstructural protein 1; cross-reactivity; false positives flavivirus; diagnostics; nonstructural protein 1; cross-reactivity; false positives
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MDPI and ACS Style

Tan, L.K.; Wong, W.Y.; Yang, H.T.; Huber, R.G.; Bond, P.J.; Ng, L.C.; Maurer-Stroh, S.; Hapuarachchi, H.C. Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays. Diagnostics 2020, 10, 11. https://doi.org/10.3390/diagnostics10010011

AMA Style

Tan LK, Wong WY, Yang HT, Huber RG, Bond PJ, Ng LC, Maurer-Stroh S, Hapuarachchi HC. Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays. Diagnostics. 2020; 10(1):11. https://doi.org/10.3390/diagnostics10010011

Chicago/Turabian Style

Tan, Li K., Wing Y. Wong, Hui T. Yang, Roland G. Huber, Peter J. Bond, Lee C. Ng, Sebastian Maurer-Stroh, and Hapuarachchige C. Hapuarachchi. 2020. "Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays" Diagnostics 10, no. 1: 11. https://doi.org/10.3390/diagnostics10010011

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