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Impact of Rearing Conditions on the Ambrosia Beetle’s Microbiome

Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Xalapa C.P. 91070, Mexico
Cátedras CONACyT. Instituto de Ecología, A. C., Carretera Antigua a Coatepec 351, Xalapa C.P. 91070, Mexico
Red de Biodiversidad y Sistemática, Instituto de Ecología A. C., Xalapa C.P. 91070, Mexico
Joint Unit of Research in Genomics and Health, Foundation for the Promotion of Health and Biomedical Research in the Valencian Community (FISABIO), 46010 Valencia, Spain
Institute for Integrative System Biology, University of Valencia-CSIC, 46010 Valencia, Spain
Tropical Research and Education Center, University of Florida, Homestead, FL 33031, USA
Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria, Unidad Integral de Diagnóstico, Servicios y Constatación, Tecámac, Estado de Mexico 55740, Mexico
Authors to whom correspondence should be addressed.
Received: 23 October 2018 / Revised: 5 December 2018 / Accepted: 7 December 2018 / Published: 13 December 2018
(This article belongs to the Special Issue Evolution of Mutualistic Symbiosis)
PDF [3128 KB, uploaded 13 December 2018]


Ambrosia beetles, along with termites and leafcutter ants, are the only fungus-farming lineages within the tree of life. Bacteria harbored by ambrosia beetles may play an essential role in the nutritional symbiotic interactions with their associated fungi; however, little is known about the impact of rearing conditions on the microbiota of ambrosia beetles. We have used culture-independent methods to explore the effect of rearing conditions on the microbiome associated with Xyleborus affinis, Xyleborus bispinatus, and Xyleborus volvulus, evaluating different media in laboratory-controlled conditions and comparing wild and laboratory conditions. Our results revealed that rearing conditions affected the fungal and bacterial microbiome structure and had a strong influence on bacterial metabolic capacities. We propose that the rearing conditions influence the ambrosia-associated fungal and bacterial communities. Furthermore, bacterial microbiome flexibility may help beetles adapt to different substrates. View Full-Text
Keywords: Xyleborus sp.; metabolic capabilities; metagenomics; microbiota; fungus Xyleborus sp.; metabolic capabilities; metagenomics; microbiota; fungus

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Ibarra-Juarez, L.A.; Desgarennes, D.; Vázquez-Rosas-Landa, M.; Villafan, E.; Alonso-Sánchez, A.; Ferrera-Rodríguez, O.; Moya, A.; Carrillo, D.; Cruz, L.; Carrión, G.; López-Buenfil, A.; García-Avila, C.; Ibarra-Laclette, E.; Lamelas, A. Impact of Rearing Conditions on the Ambrosia Beetle’s Microbiome. Life 2018, 8, 63.

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