Consequences of Decreased Light Harvesting Capability on Photosystem II Function in Synechocystis sp. PCC 6803
Abstract
:1. Introduction
2. Experimental Section
2.1. Strains and Growth Conditions
2.2. Low-Temperature Fluorescence Spectroscopy
2.3. PSI Measurements
2.4. Steady State Oxygen Evolution Measurements
2.5. Western Blots
2.6. Flash-Induced Oxygen Evolution
3. Results
3.1. Low-Temperature Fluorescence
3.2. Photosystem Stoichiometry and PSI Quantitation
3.3. Measurement of PSII Activity
3.4. Flash-Induced Oxygen Evolution
WT | CB | CK | PAL | |
---|---|---|---|---|
Km | 302.3 | 1345.2 | 1426.2 | 2864.5 |
Vmax | 312.5 | 416.7 | 1111.1 | 1666.7 |
S0 | 34.9 | 33.7 | 36.3 | 31.7 |
S1 | 42.5 | 39.1 | 40.5 | 49.3 |
S2 | 12.8 | 14.6 | 10.7 | 11.6 |
S3 | 9.8 | 12.6 | 12.4 | 7.4 |
Misses | 10.6 | 14.3 | 24.4 | 24.9 |
Single Hits | 86.7 | 82.3 | 70.0 | 68.7 |
Double Hits | 4.9 | −0.9 | 3.0 | 2.6 |
Deactivation | −1.5 | 3.8 | 3.2 | 3.7 |
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Nagarajan, A.; Page, L.E.; Liberton, M.; Pakrasi, H.B. Consequences of Decreased Light Harvesting Capability on Photosystem II Function in Synechocystis sp. PCC 6803. Life 2014, 4, 903-914. https://doi.org/10.3390/life4040903
Nagarajan A, Page LE, Liberton M, Pakrasi HB. Consequences of Decreased Light Harvesting Capability on Photosystem II Function in Synechocystis sp. PCC 6803. Life. 2014; 4(4):903-914. https://doi.org/10.3390/life4040903
Chicago/Turabian StyleNagarajan, Aparna, Lawrence E. Page, Michelle Liberton, and Himadri B. Pakrasi. 2014. "Consequences of Decreased Light Harvesting Capability on Photosystem II Function in Synechocystis sp. PCC 6803" Life 4, no. 4: 903-914. https://doi.org/10.3390/life4040903