Evidence for a Strong Relationship between the Cytotoxicity and Intracellular Location of β-Amyloid
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Purification and Preparation of Aβ42 and tAβ42 Peptides
2.3. Cell Culture and Cytotoxicity Assay
2.4. Immunocytochemistry
2.5. Measurement of Caspase Activity
2.6. Western Blot Analysis
2.7. Fibrillogenesis
2.8. Analysis of Secondary Structure
2.9. Transmission Electron Microscopy (TEM)
3. Results
3.1. Cytotoxicity of Wild-Type Aβ42 and tAβ42
3.2. Internalization of Aβ42 into the Cells
3.3. Effect of the Inhibition of Aβ42 Cytotoxicity on Cell Viability and the Internalization of Aβ42 into Cells
3.4. Lamin Protein Fragmentation and Caspase Activation Induced by tAβ42
3.5. Structural Characterization of tAB42
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Haque, M.A.; Hossain, M.S.; Bilkis, T.; Islam, M.I.; Park, I.-S. Evidence for a Strong Relationship between the Cytotoxicity and Intracellular Location of β-Amyloid. Life 2022, 12, 577. https://doi.org/10.3390/life12040577
Haque MA, Hossain MS, Bilkis T, Islam MI, Park I-S. Evidence for a Strong Relationship between the Cytotoxicity and Intracellular Location of β-Amyloid. Life. 2022; 12(4):577. https://doi.org/10.3390/life12040577
Chicago/Turabian StyleHaque, Md. Aminul, Md. Selim Hossain, Tahmina Bilkis, Md. Imamul Islam, and Il-Seon Park. 2022. "Evidence for a Strong Relationship between the Cytotoxicity and Intracellular Location of β-Amyloid" Life 12, no. 4: 577. https://doi.org/10.3390/life12040577