Maternal Gestational Diabetes Influences DNA Methylation in the Serotonin System in the Human Placenta
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Placental Biopsy
2.3. Genomic DNA Extraction and Methylation Analysis
2.4. Total RNA Extraction and Gene Expression Analysis
2.5. Statistical Analysis
3. Results
3.1. Study Participants
3.2. Methylation Levels of SLC6A4 and HTR2A in Placental Tissues
3.3. mRNA Expression of the Serotonin System
3.4. Correlations between Placental HTR2A and SLC6A4 Methylation Levels and Clinical Factors
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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GDM (N = 30) | Control (N = 60) | p-Value | |
---|---|---|---|
Maternal characteristics | |||
Maternal age, years | 35.77 ± 2.89 | 33.68 ± 4.06 | 0.014 * |
Nulliparous, % | 15 (50) | 40 (66) | 0.262 |
Prepregnancy BMI, kg/m2 | 26.84 ± 4.52 | 22.07 ± 4.04 | <0.001 * |
Gestation at birth, weeks | 36.91 ± 2.46 | 37.13 ± 2.31 | 0.683 |
Delivery < 37 weeks, % | 10 (33.3) | 18 (30) | 0.747 |
Gestational weight gain | 10.97 ± 7.58 | 13.75 ± 5.65 | 0.054 |
% low category | 13 (43.3) | 19 (31.7) | 0.548 |
% normal category | 9 (30.0) | 21 (35.0) | |
% high category | 8 (28.6) | 20 (33.3) | |
Fasting glucose, mg/dL | 96.47 ± 18.59 | 86.62 ± 15.62 | 0.01 * |
50 g OGTT | 186.06 ± 30.03 | 122.70 ± 21.61 | < 0.001 * |
Total cholesterol | 256.07 ± 41.85 | 267.26 ± 50.75 | 0.431 |
Neonate and placenta characteristics | |||
Birth weight, kg | 3.03 ± 0.76 | 2.86 ± 0.72 | 0.3 |
Birthweight percentile | 54.63 ± 28.53 | 40.18 ± 26.28 | 0.019 * |
SGA, % | 1 (3.3) | 6 (10.0) | 0.205 |
LGA, % | 5 (16.7) | 4 (6.7) | |
Apgar < 7 at 5 min, % | 1 (3.3) | 2 (3.3) | 1 |
Infant gender (% male) | 13 (43.3) | 33 (55.0) | 0.297 |
Fasting glucose | 79.14 ± 55.11 | 64.25 ± 28.51 | 0.21 |
TSH | 3.63 ± 2.17 | 3.77 ± 2.14 | 0.769 |
17alpha-OHP | 1.83 ± 1.15 | 2.04 ± 1.20 | 0.433 |
Placenta weight, kg | 0.70 ± 0.21 | 0.64 ± 0.20 | 0.192 |
Placental weight < 10th percentile | 2 (6.7) | 9 (15.0) | 0.255 |
β | p | R2 | |
---|---|---|---|
Mean methylation levels of SLC6A4 (%) | |||
Fasting plasma glucose | 0.007 | 0.036 * | 0.196 |
Birthweight percentile | 0.008 | 0.002 * | 0.197 |
HC percentile | −0.006 | 0.017 * | 0.226 |
Mean methylation levels of HTR2A (%) | |||
Fasting plasma glucose | 0.037 | 0.132 | 0.061 |
Birthweight percentile | 0.015 | 0.441 | 0.006 |
HC percentile | −0.018 | 0.350 | 0.072 |
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Song, J.Y.; Lee, K.E.; Byeon, E.J.; Choi, J.; Kim, S.J.; Shin, J.E. Maternal Gestational Diabetes Influences DNA Methylation in the Serotonin System in the Human Placenta. Life 2022, 12, 1869. https://doi.org/10.3390/life12111869
Song JY, Lee KE, Byeon EJ, Choi J, Kim SJ, Shin JE. Maternal Gestational Diabetes Influences DNA Methylation in the Serotonin System in the Human Placenta. Life. 2022; 12(11):1869. https://doi.org/10.3390/life12111869
Chicago/Turabian StyleSong, Jae Yen, Kyung Eun Lee, Eun Jeong Byeon, Jieun Choi, Sa Jin Kim, and Jae Eun Shin. 2022. "Maternal Gestational Diabetes Influences DNA Methylation in the Serotonin System in the Human Placenta" Life 12, no. 11: 1869. https://doi.org/10.3390/life12111869