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A Severe Acute Pancreatitis Mouse Model Transited from Mild Symptoms Induced by a “Two-Hit” Strategy with L-Arginine

Department of Pharmaceutics, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China
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Author to whom correspondence should be addressed.
Academic Editors: Xingxin Wu, Toshimi Chiba, Jie Yan, Emanuele Sinagra and Manuel Perucho
Life 2022, 12(1), 126; https://doi.org/10.3390/life12010126
Received: 30 December 2021 / Revised: 13 January 2022 / Accepted: 14 January 2022 / Published: 16 January 2022
(This article belongs to the Topic Inflammation and Fibrosis in Digestive Diseases)
To develop a severe acute pancreatitis (SAP) model transited from mild symptoms, we investigated a “two-hit” strategy with L-arginine in mice. The mice were intraperitoneally injected with ice-cold L-arginine (4 g/kg) twice at an interval of 1 h on the first day and subjected to the repeated operation 72 h afterwards. The results showed the “two-hit” strategy resulted in the destructive damage and extensive necrosis of acinar cells in the pancreas compared with the “one-hit” model. Meanwhile, excessive levels of pro-inflammatory mediators, namely IL-6 and TNF-α, were released in the serum. Remarkably, additional deleterious effects on multiple organs were observed, including high intestinal permeability, kidney injury, and severe acute lung injury. Therefore, we confirmed that the SAP animal model triggered by a “two-hit” strategy with L-arginine was successfully established, providing a solid foundation for a deeper understanding of SAP initiation and therapy research to prevent worsening of the disease. View Full-Text
Keywords: L-arginine; severe acute pancreatitis; two-hit; multi-organ failure; mouse model L-arginine; severe acute pancreatitis; two-hit; multi-organ failure; mouse model
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MDPI and ACS Style

Yang, J.; Tang, X.; Wu, Q.; Ren, P.; Yan, Y. A Severe Acute Pancreatitis Mouse Model Transited from Mild Symptoms Induced by a “Two-Hit” Strategy with L-Arginine. Life 2022, 12, 126. https://doi.org/10.3390/life12010126

AMA Style

Yang J, Tang X, Wu Q, Ren P, Yan Y. A Severe Acute Pancreatitis Mouse Model Transited from Mild Symptoms Induced by a “Two-Hit” Strategy with L-Arginine. Life. 2022; 12(1):126. https://doi.org/10.3390/life12010126

Chicago/Turabian Style

Yang, Jing, Xujiao Tang, Qingqing Wu, Panpan Ren, and Yishu Yan. 2022. "A Severe Acute Pancreatitis Mouse Model Transited from Mild Symptoms Induced by a “Two-Hit” Strategy with L-Arginine" Life 12, no. 1: 126. https://doi.org/10.3390/life12010126

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