Measuring Proviral HIV-1 DNA: Hurdles and Improvements to an Assay Monitoring Integration Events Utilising Human Alu Repeat Sequences
Abstract
:1. Introduction
2. Materials and Methods
2.1. Integrated HIV-1 DNA Quantification by qPCR
2.2. Patients Tested with the Alu-gag and Alu-LTR Assay
2.3. Standard for Integrated HIV-1 DNA Assay
2.4. Statistics
3. Results
3.1. Design an Improved HIV-1 Integration Assay (Alu-5LTR)
3.2. Testing of Alu-5LTR in Patients with Undetectable Viral Load Undergoing Suppressive Antiretroviral Therapy
3.3. Bland Altman Analysis of the Alu-5LTR vs. Alu-gag Assays
3.4. Testing of a Previously Used HIV-1 DNA Standard to Define the Lower Input Needed to Allow Reliable Poisson Quantification
3.5. Testing of a Cellular Standard to Be Used for the Initial Setting of the Alu-5LTR Assay: Towards Cross-Laboratory Harmonisation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Malatinkova, E.; Thomas, J.; De Spiegelaere, W.; Rutsaert, S.; Geretti, A.M.; Pollakis, G.; Paxton, W.A.; Vandekerckhove, L.; Ruggiero, A. Measuring Proviral HIV-1 DNA: Hurdles and Improvements to an Assay Monitoring Integration Events Utilising Human Alu Repeat Sequences. Life 2021, 11, 1410. https://doi.org/10.3390/life11121410
Malatinkova E, Thomas J, De Spiegelaere W, Rutsaert S, Geretti AM, Pollakis G, Paxton WA, Vandekerckhove L, Ruggiero A. Measuring Proviral HIV-1 DNA: Hurdles and Improvements to an Assay Monitoring Integration Events Utilising Human Alu Repeat Sequences. Life. 2021; 11(12):1410. https://doi.org/10.3390/life11121410
Chicago/Turabian StyleMalatinkova, Eva, Jordan Thomas, Ward De Spiegelaere, Sofie Rutsaert, Anna Maria Geretti, Georgios Pollakis, William A. Paxton, Linos Vandekerckhove, and Alessandra Ruggiero. 2021. "Measuring Proviral HIV-1 DNA: Hurdles and Improvements to an Assay Monitoring Integration Events Utilising Human Alu Repeat Sequences" Life 11, no. 12: 1410. https://doi.org/10.3390/life11121410