SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo
Abstract
:1. Introduction
2. Methods
3. Results
3.1. Endometrial Receptivity Assessment Using the Win-Test during the Implantation Window
3.2. Endometrial SARS-CoV-2-Associated Receptors and Proteases during the Implantation Window under Natural and Stimulated Cycles
3.3. SARS-CoV-2-Associated Receptors and Proteases in Human Preimplantation Embryos
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Endometrial Tissus | |||||||||
---|---|---|---|---|---|---|---|---|---|
Natural Cycle | Stimulated Cycle | ||||||||
ES Phase (n = 31) | MS Phase (n = 31) | Frequency Distribution | ES Phase (n = 21) | MS Phase (n = 21) | Frequency Distribution | ||||
Gene Name | Gene ID | No. of Patients (%) | No. of Patients (%) | p-Value | Signal Intensity | No. of Patients (%) | No. of Patients (%) | p-Value | Signal Intensity |
ACE | 227463_at | 19 (61.3) | 16 (51.6) | ns | + | 10 (47.7) | 10 (47.7) | ns | + |
ACE2 | 219962_at | 13 (41.9) | 25 (80.6) | 0.0005 | +/− | 11 (52.4) | 17 (80.9) | 0.015 | +/− |
222257_s_at | 1 (3.2) | 10 (32.3) | 0.0009 | +/− | 2 (9.5) | 10 (47.7) | 0.002 | +/− | |
AGT | 202834_at | 28 (90.3) | 26 (83.9) | ns | +/− | 21 (100) | 19 (90.5) | ns | +/− |
AGTR1 | 205357_s_at | 22 (71.0) | 30 (96.8) | 0.008 | +/− | 19 (90.5) | 21 (100) | ns | +/− |
AGTR2 | 207294_at | 11 (35.5) | 4 (12.9) | 0.02 | +/− | 1 (4.8) | 0 | +/− | |
ANPEP | 202888_s_at | 31 (100) | 31 (100) | ns | +/− | 21 (100) | 21 (100) | ns | + |
CPA3 | 205624_at | 9 (29.0) | 11 (35.5) | ns | +/− | 16 (76.2) | 14 (66.7) | ns | +/− |
CTSA | 200661_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ |
ENPEP | 204844_at | 30 (96.8) | 30 (96.8) | ns | +/− | 21 (100) | 21 (100) | ns | + |
204845_s_at | 28 (90.3) | 29 (93.5) | ns | +/− | 21 (100) | 18 (85.7) | ns | +/− | |
LNPEP | 207904_s_at | 22 (71.0) | 26 (83.9) | ns | +/− | 12 (57.1) | 11 (52.4) | ns | +/− |
225176_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ | |
231866_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | + | |
236728_at | 27 (87.1) | 24 (77.4) | ns | +/− | 21 (100) | 17 (80.9) | 0.036 | +/− | |
MME | 203434_s_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ |
203435_s_at | 30 (96.8) | 29 (93.5) | ns | ++ | 21 (100) | 21 (100) | ns | ++ | |
NLN | 224063_at | 3 (9.7) | 3 (9.7) | ns | +/− | 2 (9.5) | 3 (14.3) | ns | +/− |
225942_at | 22 (71.0) | 23 (74.2) | ns | +/− | 13 (61.9) | 12 (57.1) | ns | +/− | |
225943_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | + | |
225944_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | + | |
234762_x_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ | |
THOP1 | 203235_at | 31 (100) | 31 (100) | ns | +/− | 21 (100) | 20 (95.2) | ns | +/− |
TMPRSS2 | 226553_at | 19 (61.3) | 14 (45.2) | ns | +/− | 11 (52.4) | 7 (33.3) | 0.042 | +/− |
BSG | 208677_s_at | 29 (93.5) | 30 (96.8) | ns | ++ | 19 (90.5) | 20 (95.2) | ns | ++ |
CTSL | 202087_s_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ |
FURIN | 201945_at | 16 (51.6) | 19 (61.3) | ns | +/− | 13 (61.9) | 15 (71.4) | ns | +/− |
GAPDH | M33197_M_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ |
ACTB | X00351_5_at | 31 (100) | 31 (100) | ns | ++ | 21 (100) | 21 (100) | ns | ++ |
STAT1 | M97935_3_at | 31 (100) | 31 (100) | ns | +/− | 21 (100) | 21 (100) | ns | +/− |
ACE2-TMPRSS2 | ACE2-BSG | ACE2-CTSL | ACE2-CTSA | ACE2-FURIN | BSG-TMPRSS2 | BSG-CTSL | BSG-CTSA | BSG-FURIN | |
---|---|---|---|---|---|---|---|---|---|
20 (32.3) | 37 (59.7) | 38 (61.3) | 38 (61.3) | 25 (40.3) | 30 (48.4) | 59 (95.2) | 59 (95.2) | 34 (54.8) | Endometrium (n = 62) |
4 (40) | 10 (100) | 10 (100) | 10 (100) | 10 (100) | 4 (40) | 10 (100) | 10 (100) | 10 (100) | Bl (n = 10) |
0 | 0 | 1 (20) | 0 | 1 (20) | 2 (40) | 3 (60) | 2 (40) | 1 (20) | TE (n = 5) |
Blastocyst | Trophectoderm | ||||
---|---|---|---|---|---|
Gene Name | Gene ID | No. of Samples (%) | Signal Intensity | No. of Samples (%) | Signal Intensity |
ACE2 | 219962_at | 10 (100) | ++ | 1 (20) | +/− |
222257_s_at | 10 (100) | ++ | 2 (40) | + | |
TMPRSS2 | 226553_at | 4 (40) | ++ | 3 (60) | + |
BSG | 208677_s_at | 10 (100) | ++ | 3 (60) | +/− |
CTSL | 202087_s_at | 10 (100) | ++ | 5 (100) | ++ |
CTSA | 200661_at | 10 (100) | ++ | 2 (40) | +/− |
FURIN | 201945_at | 10 (100) | + | 2 (40) | +/− |
GAPDH | M33197_M_at | 10 (100) | ++ | 5 (100) | ++ |
ACTB | X00351_5_at | 10 (100) | + | 5 (100) | ++ |
STAT1 | M97935_3_at | 10 (100) | +/− | 5 (100) | +/− |
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Haouzi, D.; Entezami, F.; Tuaillon, E.; Gala, A.; Ferrières-Hoa, A.; Brouillet, S.; Thierry, A.R.; Hamamah, S. SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo. Life 2021, 11, 1378. https://doi.org/10.3390/life11121378
Haouzi D, Entezami F, Tuaillon E, Gala A, Ferrières-Hoa A, Brouillet S, Thierry AR, Hamamah S. SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo. Life. 2021; 11(12):1378. https://doi.org/10.3390/life11121378
Chicago/Turabian StyleHaouzi, Delphine, Frida Entezami, Edward Tuaillon, Anna Gala, Alice Ferrières-Hoa, Sophie Brouillet, Alain R. Thierry, and Samir Hamamah. 2021. "SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo" Life 11, no. 12: 1378. https://doi.org/10.3390/life11121378