Tomentosin a Sesquiterpene Lactone Induces Antiproliferative and Proapoptotic Effects in Human Burkitt Lymphoma by Deregulation of Anti- and Pro-Apoptotic Genes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Cytotoxicity Assay
2.3. Apoptotic Assay
2.4. Cell Cycle Analysis
2.5. Gene Expression Analysis
2.6. Functional Classification and Pathway Analysis of DEGs
2.7. Statistical Data Analysis
3. Results
3.1. Effects of Tomentosin on Cell Viability in Human Burkitt Lymphoma Cells
3.2. Effects of Tomentosin on Apoptotic Cellular Processes in Human Burkitt Lymphoma Cells
3.3. Effects of Tomentosin on Cell Cycle in Human Burkitt Lymphoma Cells
3.4. Effects of Tomentosin on Gene Expression Profiling in Human Burkitt Lymphoma Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Cell Line | Treatment | IC50 | Confidence Interval 95% |
---|---|---|---|
Raji | Tomentosin | 42.62 µM | 39.53–46.47 µM |
MRC5 | Tomentosin | >50.00 µM | — |
Raji | Cispaltin | 9.43 µM | 8.23–11.21 µM |
MRC5 | Cispaltin | 14.77 µM | 13.1–16.48 µM |
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Virdis, P.; Marchesi, I.; Fiorentino, F.P.; Migheli, R.; Sanna, L.; Bordoni, V.; Pintore, G.; Galleri, G.; Muroni, M.R.; Bagella, L.; et al. Tomentosin a Sesquiterpene Lactone Induces Antiproliferative and Proapoptotic Effects in Human Burkitt Lymphoma by Deregulation of Anti- and Pro-Apoptotic Genes. Life 2021, 11, 1128. https://doi.org/10.3390/life11111128
Virdis P, Marchesi I, Fiorentino FP, Migheli R, Sanna L, Bordoni V, Pintore G, Galleri G, Muroni MR, Bagella L, et al. Tomentosin a Sesquiterpene Lactone Induces Antiproliferative and Proapoptotic Effects in Human Burkitt Lymphoma by Deregulation of Anti- and Pro-Apoptotic Genes. Life. 2021; 11(11):1128. https://doi.org/10.3390/life11111128
Chicago/Turabian StyleVirdis, Patrizia, Irene Marchesi, Francesco Paolo Fiorentino, Rossana Migheli, Luca Sanna, Valentina Bordoni, Giorgio Pintore, Grazia Galleri, Maria Rosaria Muroni, Luigi Bagella, and et al. 2021. "Tomentosin a Sesquiterpene Lactone Induces Antiproliferative and Proapoptotic Effects in Human Burkitt Lymphoma by Deregulation of Anti- and Pro-Apoptotic Genes" Life 11, no. 11: 1128. https://doi.org/10.3390/life11111128