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Article

Whole Genome Sequencing and Comparative Genome Analysis of the Halotolerant Deep Sea Black Yeast Hortaea werneckii

1
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy
2
Department of Clinical and Experimental Medicine, University Hospital of Messina, 98125 Messina, Italy
*
Author to whom correspondence should be addressed.
Life 2020, 10(10), 229; https://doi.org/10.3390/life10100229
Received: 14 September 2020 / Revised: 25 September 2020 / Accepted: 30 September 2020 / Published: 2 October 2020
(This article belongs to the Special Issue Advances in Fungal -Omics)
Hortaea werneckii, an extreme halotolerant black yeast in the order of Capnodiales, was recently isolated from different stations and depths in the Mediterranean Sea, where it was shown to be the dominant fungal species. In order to explore the genome characteristics of these Mediterranean isolates, we carried out a de-novo sequencing of the genome of one strain isolated at a depth of 3400 m (MC873) and a re-sequencing of one strain taken from a depth of 2500 m (MC848), whose genome was previously sequenced but was highly fragmented. A comparative phylogenomic analysis with other published H. werneckii genomes was also carried out to investigate the evolution of the strains from the deep sea in this environment. A high level of genome completeness was obtained for both genomes, for which genome duplication and an extensive level of heterozygosity (~4.6%) were observed, supporting the recent hypothesis that a genome duplication caused by intraspecific hybridization occurred in most H. werneckii strains. Phylogenetic analyses showed environmental and/or geographical specificity, suggesting a possible evolutionary adaptation of marine H. werneckii strains to the deep sea environment. We release high-quality genome assemblies from marine H. werneckii strains, which provides additional data for further genomics analysis, including niche adaptation, fitness and evolution studies. View Full-Text
Keywords: Hortaea werneckii; black yeasts; whole-genome sequencing; genome assembly; annotation Hortaea werneckii; black yeasts; whole-genome sequencing; genome assembly; annotation
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MDPI and ACS Style

Romeo, O.; Marchetta, A.; Giosa, D.; Giuffrè, L.; Urzì, C.; De Leo, F. Whole Genome Sequencing and Comparative Genome Analysis of the Halotolerant Deep Sea Black Yeast Hortaea werneckii. Life 2020, 10, 229. https://doi.org/10.3390/life10100229

AMA Style

Romeo O, Marchetta A, Giosa D, Giuffrè L, Urzì C, De Leo F. Whole Genome Sequencing and Comparative Genome Analysis of the Halotolerant Deep Sea Black Yeast Hortaea werneckii. Life. 2020; 10(10):229. https://doi.org/10.3390/life10100229

Chicago/Turabian Style

Romeo, Orazio, Alessia Marchetta, Domenico Giosa, Letterio Giuffrè, Clara Urzì, and Filomena De Leo. 2020. "Whole Genome Sequencing and Comparative Genome Analysis of the Halotolerant Deep Sea Black Yeast Hortaea werneckii" Life 10, no. 10: 229. https://doi.org/10.3390/life10100229

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