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Open AccessArticle

Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile Acidithiobacillus ferrooxidans Strain DC

by Bo Miao 1,2, Li Shen 1,2, Xueduan Liu 1,2, Weimin Zeng 1,2 and Xueling Wu 1,2,*
1
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
2
Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China
*
Author to whom correspondence should be addressed.
Minerals 2020, 10(6), 544; https://doi.org/10.3390/min10060544
Received: 30 April 2020 / Revised: 8 June 2020 / Accepted: 13 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Surface Chemistry in Mineral Processing and Extractive Metallurgy)
The family of Nramp (natural resistance-associated macrophage protein) metal ion transporter functions in diverse organisms from bacteria to humans. Acidithiobacillus ferrooxidans (At. ferrooxidans) is a Gram-negative bacterium that lives at pH 2 in high concentrations of soluble ferrous ion (600 mM). The AFE_2126 protein of At. ferrooxidans of the Dachang Copper Mine (DC) was analyzed by bioinformatics software or online tools, showing that it was highly homologous to the Nramp family, and its subcellular localization was predicted to locate in the cytoplasmic membrane. Transcriptional study revealed that AFE_2126 was expressed by Fe2+-limiting conditions in At. ferrooxidans DC. It can be concluded that the AFE_2126 protein may function in ferrous ion transport into the cells. Based on the ΔpH of the cytoplasmic membrane between the periplasm (pH 3.5) and the cytoplasm (pH 6.5), it can be concluded that Fe2+ is transported in the direction identical to that of the H+ gradient. This study indirectly confirmed that the function of Nramp in At. ferrooxidans DC can transport divalent iron ions. View Full-Text
Keywords: acidophilic bacterium; AFE_2126; Nramp family; metal ion transporters; magnetic nanoparticles; multireference genes acidophilic bacterium; AFE_2126; Nramp family; metal ion transporters; magnetic nanoparticles; multireference genes
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Miao, B.; Shen, L.; Liu, X.; Zeng, W.; Wu, X. Bioinformatics and Transcriptional Study of the Nramp Gene in the Extreme Acidophile Acidithiobacillus ferrooxidans Strain DC. Minerals 2020, 10, 544.

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