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Antibodies 2018, 7(3), 24; https://doi.org/10.3390/antib7030024

Engaging with Raman Spectroscopy to Investigate Antibody Aggregation

Department of Chemistry, Lancaster University, Lancaster, Lancashire LA1 4YB, UK
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Received: 18 June 2018 / Revised: 4 July 2018 / Accepted: 4 July 2018 / Published: 7 July 2018
(This article belongs to the Special Issue Aggregation of Antibodies)
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Abstract

In the last decade, a number of studies have successfully demonstrated Raman spectroscopy as an emerging analytical technique for monitoring antibody aggregation, especially in the context of drug development and formulation. Raman spectroscopy is a robust method for investigating protein conformational changes, even in highly concentrated antibody solutions. It is non-destructive, reproducible and can probe samples in an aqueous environment. In this review, we focus on the application and challenges associated with using Raman spectroscopy as a tool to study antibody aggregates. View Full-Text
Keywords: Raman spectroscopy; antibody; aggregation; protein structure Raman spectroscopy; antibody; aggregation; protein structure
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ettah, I.; Ashton, L. Engaging with Raman Spectroscopy to Investigate Antibody Aggregation. Antibodies 2018, 7, 24.

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