Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of NS1-Specific DENV mAbs
2.2. Detection of Native DENV2 NS1 and Epitope Mapping
2.3. Analyses of mAbs’ Cross-Reactivity with Different DENV Serotype and ZIKV
3. Discussion
4. Materials and Methods
4.1. Viral Strains and Viral Antigen
4.2. Dengue NS1 Monoclonal Antibody (mAb) Production
4.3. Dengue NS1 mAbs Characterization
4.4. Epitope Characterization and Structure Analysis
4.5. NS1 Sequences Database Building
4.6. Conservancy Analysis
4.7. NS1 mAbs Reactivity to Dengue Virus Serotypes and Zika Virus
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Name | 4F6 | 4H2 | 2H5 | 4H1BC |
---|---|---|---|---|
IgG Subtype a | IgG2a | IgG2a | IgG1 | IgG1 |
DENV2 NS1 reactivity b | Yes | Yes | Yes | Yes |
Dissociation Constant (KD) c | 1.1 × 10−8 M | 6.2 × 10−8 M | 7.3 × 10−8 M | 8.4 × 10−8 M |
Detection limit d | 16 ng/mL | 32 ng/mL | 32 ng/mL | 32 ng/mL |
Epitope sequence e | 25VHTWTEQYKFQPES38 | 127ELHNQTFLIDGPETAEC143 | 193AVHADMGYWIESALNDT209 | 193AVHADMGYWIESALNDT209 |
DENV (1–4) reactivity f | No | Yes | No | No |
ZIKV reactivity g | No | No | Yes | Yes |
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Rocha, L.B.; Alves, R.P.d.S.; Caetano, B.A.; Pereira, L.R.; Mitsunari, T.; Amorim, J.H.; Polatto, J.M.; Botosso, V.F.; Gallina, N.M.F.; Palacios, R.; et al. Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies. Antibodies 2017, 6, 14. https://doi.org/10.3390/antib6040014
Rocha LB, Alves RPdS, Caetano BA, Pereira LR, Mitsunari T, Amorim JH, Polatto JM, Botosso VF, Gallina NMF, Palacios R, et al. Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies. Antibodies. 2017; 6(4):14. https://doi.org/10.3390/antib6040014
Chicago/Turabian StyleRocha, Leticia Barboza, Rubens Prince dos Santos Alves, Bruna Alves Caetano, Lennon Ramos Pereira, Thais Mitsunari, Jaime Henrique Amorim, Juliana Moutinho Polatto, Viviane Fongaro Botosso, Neuza Maria Frazatti Gallina, Ricardo Palacios, and et al. 2017. "Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies" Antibodies 6, no. 4: 14. https://doi.org/10.3390/antib6040014
APA StyleRocha, L. B., Alves, R. P. d. S., Caetano, B. A., Pereira, L. R., Mitsunari, T., Amorim, J. H., Polatto, J. M., Botosso, V. F., Gallina, N. M. F., Palacios, R., Precioso, A. R., Granato, C. F. H., Oliveira, D. B. L., Silveira, V. B. d., Luz, D., Ferreira, L. C. d. S., & Piazza, R. M. F. (2017). Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies. Antibodies, 6(4), 14. https://doi.org/10.3390/antib6040014