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Antibodies 2017, 6(4), 14; https://doi.org/10.3390/antib6040014

Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies

1
Laboratório de Bacteriologia, Instituto Butantan, São Paulo, 05503-900 SP, Brazil
2
Laboratório de Desenvolvimento de Vacinas, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 05508-000, SP, Brazil
3
Laboratório de Virologia, Instituto Butantan, São Paulo, 05503-900 SP, Brazil
4
Divisão de Desenvolvimento Tecnológico e Produção; Instituto Butantan, São Paulo, 05503-900, SP, Brazil
5
Divisão de Ensaios Clínicos e Farmacovigilância, Instituto Butantan, São Paulo, 05503-900, SP, Brazil
6
Departamento de Medicina, Disciplina de Doenças Infecciosas e Parasitárias, Universidade Federal de São Paulo, São Paulo, 04023-062, SP, Brazil
7
Laboratório de Virologia Molecular e Clínica, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 05508-000, SP, Brazil
These authors contributed equally to the present work.
Present address: Laboratório de Microbiologia, Centro das Ciências Biológicas e da Saúde, Universidade Federal do Oeste da Bahia, Barreiras, 47805-100. Bahia, Brazil.
*
Author to whom correspondence should be addressed.
Received: 8 August 2017 / Revised: 22 September 2017 / Accepted: 22 September 2017 / Published: 15 October 2017
(This article belongs to the Special Issue Monoclonal Antibodies)
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Abstract

Dengue nonstructural protein 1 (NS1) is a multi-functional glycoprotein with essential functions both in viral replication and modulation of host innate immune responses. NS1 has been established as a good surrogate marker for infection. In the present study, we generated four anti-NS1 monoclonal antibodies against recombinant NS1 protein from dengue virus serotype 2 (DENV2), which were used to map three NS1 epitopes. The sequence 193AVHADMGYWIESALNDT209 was recognized by monoclonal antibodies 2H5 and 4H1BC, which also cross-reacted with Zika virus (ZIKV) protein. On the other hand, the sequence 25VHTWTEQYKFQPES38 was recognized by mAb 4F6 that did not cross react with ZIKV. Lastly, a previously unidentified DENV2 NS1-specific epitope, represented by the sequence 127ELHNQTFLIDGPETAEC143, is described in the present study after reaction with mAb 4H2, which also did not cross react with ZIKV. The selection and characterization of the epitope, specificity of anti-NS1 mAbs, may contribute to the development of diagnostic tools able to differentiate DENV and ZIKV infections. View Full-Text
Keywords: dengue virus; NS1; Zika virus; mAbs; antibody recognition; amino acid sequences dengue virus; NS1; Zika virus; mAbs; antibody recognition; amino acid sequences
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rocha, L.B.; Alves, R.P.S.; Caetano, B.A.; Pereira, L.R.; Mitsunari, T.; Amorim, J.H.; Polatto, J.M.; Botosso, V.F.; Gallina, N.M.F.; Palacios, R.; Precioso, A.R.; Granato, C.F.H.; Oliveira, D.B.L.; Silveira, V.B.; Luz, D.; Ferreira, L.C.S.; Piazza, R.M.F. Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies. Antibodies 2017, 6, 14.

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