Next Article in Journal
Opportunities for Conformation-Selective Antibodies in Amyloid-Related Diseases
Previous Article in Journal
Pre-Clinical Intravenous Serum Pharmacokinetics of Albumin Binding and Non-Half-Life Extended Nanobodies®
Open AccessArticle

Model-Based Comparison of Antibody Dimerization in Continuous and Batch-Wise Downstream Processing

Department of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
Author to whom correspondence should be addressed.
Academic Editor: Zivko Nikolov
Antibodies 2015, 4(3), 157-169;
Received: 31 March 2015 / Revised: 23 June 2015 / Accepted: 6 July 2015 / Published: 10 July 2015
(This article belongs to the Special Issue Optimized Antibody Recovery and Purification Methods)
Monoclonal antibodies are generally produced using a generic platform approach in which several chromatographic separations assure high purity of the product. Dimerization can occur during the fermentation stage and may occur also during the downstream processing. We present here simulations in which a traditional platform approach that consist of protein A capture, followed by cation-exchange and anion-exchange chromatography for polishing is compared to a continuous platform in which dimer removal and virus inactivation are carried out on a size-exclusion column. A dimerization model that takes pH, salt concentration and the concentration of antibodies into account is combined with chromatographic models, to be able to predicted both the separation and the degree to which dimers are formed. Purification of a feed composition that contained 1% by weight of dimer and a total antibody concentration of 1 g/L was modeled using both approaches, and the amount of antibodies in the continuous platform was at least 4 times lower than in the traditional platform. The total processing time was also lower, as the cation-exchange polish could be omitted. View Full-Text
Keywords: dimerization; downstream processing; modeling dimerization; downstream processing; modeling
Show Figures

Figure 1

MDPI and ACS Style

Sellberg, A.; Ojala, F.; Nilsson, B. Model-Based Comparison of Antibody Dimerization in Continuous and Batch-Wise Downstream Processing. Antibodies 2015, 4, 157-169.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Search more from Scilit
Back to TopTop