Next Article in Journal / Special Issue
Submerged Pond Sand Filter—A Novel Approach to Rural Water Supply
Previous Article in Journal
Hedging Rules for Water Supply Reservoir Based on the Model of Simulation and Optimization
Previous Article in Special Issue
Current Status of Marker Genes of Bacteroides and Related Taxa for Identifying Sewage Pollution in Environmental Waters
Open AccessArticle

Inter-Laboratory Evaluation and Successful Implementation of MS2 Coliphage as a Surrogate to Establish Proficiency Using a BSL-3 Procedure

1
United States Environmental Protection Agency, Office of Water, 1200 Pennsylvania Ave NW, Washington, DC 20460, USA
2
Central Savannah River Area (CSRA), Science & Engineering, 6361 Walker Lane, Suite 300, Alexandria, VA 22310, USA
3
Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, 1600 Clifton Rd NE, Mailstop D66, Atlanta, GA 30333, USA
4
IHRC, Inc., 2 Ravinia Drive NE, Atlanta, GA 30346, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Sunny Jiang and Yiping Cao
Water 2016, 8(6), 248; https://doi.org/10.3390/w8060248
Received: 15 April 2016 / Revised: 19 May 2016 / Accepted: 3 June 2016 / Published: 10 June 2016
(This article belongs to the Special Issue Pathogens in Water)
The U.S. Environmental Protection Agency’s (EPA) Water Laboratory Alliance relies on the Centers for Disease Control and Prevention’s ultrafiltration-based Water Processing Procedure (WPP) for concentration of biosafety level 3 (BSL-3) agents from 10 L to 100 L of drinking water. The WPP requires comprehensive training and practice to maintain proficiency, resulting in a critical need for quality control (QC) criteria. The aim of this study was to develop criteria using male-specific (MS2) coliphage (BSL-2 agent) to minimize safety hazards associated with BSL-3 agents and to use the criteria to evaluate analytical proficiency during a demonstration exercise. EPA Method 1602 with EasyPhage was used during the study to develop QC criteria for 100-mL, and 40–100 L samples. The demonstration exercise indicated that the MS2 criteria would allow laboratories to demonstrate proficiency using the WPP with 40–100 L samples. In addition, the QC criteria developed for 100-mL samples has broad applicability at laboratories that are using MS2 for other types of analyses, such as assessment of water treatment devices. The development of MS2 QC criteria allows laboratories to develop and confirm ongoing proficiency using the WPP. View Full-Text
Keywords: male-specific (MS2); coliphage; drinking water; quality control criteria; ultrafiltration male-specific (MS2); coliphage; drinking water; quality control criteria; ultrafiltration
MDPI and ACS Style

Mapp, L.; Chambers, Y.; Takundwa, P.; Hill, V.R.; Schneeberger, C.; Knee, J.; Raynor, M.; Klonicki, P.; Miller, K.; Pope, M.; Hwang, N. Inter-Laboratory Evaluation and Successful Implementation of MS2 Coliphage as a Surrogate to Establish Proficiency Using a BSL-3 Procedure. Water 2016, 8, 248.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop