Hydraulic Characteristics and the Adaptability to Water-Level Fluctuation of the Vertical-Slot Fishway
Abstract
1. Introduction
2. Materials and Methods
2.1. The Structure of Fishway
2.2. Thresholds of Target Fish Species to the Hydraulic Characteristics
2.3. Numerical Model
3. Results
3.1. Water Depth Profiles
3.2. Velocity in the Slots
3.3. Turbulent Kinetic Energy
3.4. Turbulent Kinetic Energy Dissipation Rate
4. Discussion
4.1. Effects of Hydraulic Characteristics on Fish Migration Behavior
4.2. The Relationship Between Allowable Water Depth and the Distance of the Two Entrances and Its Applications
4.3. Study Limitations and Future Research Directions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- He, F.Z.; Zarfl, C.; Tockner, K.; Olden, J.D.; Campos, Z.; Muniz, F.; Svenning, J.C.; Jähnig, S.C. Hydropower impacts on riverine biodiversity. Nat. Rev. Earth Environ. 2024, 5, 755–772. [Google Scholar] [CrossRef]
- Iaia, M.; Quadroni, S.; Brignone, S.; Piccinini, A.; Bettinetti, R.; Volta, P. Assessment of the effectiveness and efficiency of two fishways with vertical slot openings in an Alpine River (Toce River, northern Italy). Ecol. Eng. 2025, 212, 107535. [Google Scholar] [CrossRef]
- Romao, F.; Quaresma, A.; Simao, J.; Amaral, S.; Leite, R.; Bravo-Córdoba, F.J.; Sanz-Ronda, F.J.; Pinheiro, A.N.; Santos, J.M. Stopping invaders: Moving towards a selective vertical slot fishway to prevent the passage of non-native cyprinids. J. Environ. Manag. 2025, 380, 125004. [Google Scholar] [CrossRef]
- Ke, S.F.; Xiang, S.; Kattel, G.R.; Li, D.Q.; Tu, Z.Y.; Shi, X.T. Design and initial evaluation of a novel tubular fishway for the rubber dam on the Huangbai River, a tributary of the Gezhouba Reservoir. J. Environ. Manag. 2025, 381, 125301. [Google Scholar] [CrossRef] [PubMed]
- Liu, S.; Jian, Y.; Li, P.; Liang, R.; Chen, X.; Qin, Y.; Wang, Y.; Li, K. Optimization schemes to significantly improve the upstream migration of fish: A case study in the lower Yangtze River basin. Ecol. Eng. 2023, 186, 106838. [Google Scholar] [CrossRef]
- Moccetti, P.; Dodd, J.R.; Joyce, D.A.; Nunn, A.D.; Gillespie, B.; Bolland, J.D. Genetic consequences of improved river connec-tivity in brown trout (Salmo trutta L.). Evol. Appl. 2024, 17, e13660. [Google Scholar] [CrossRef]
- Cui, L.; Kou, X.M.; Sun, J.J.; Liu, R.; Gao, F.; Tan, J.J.; Soomro, S.; Wang, Y.Y.; Kattel, G.R.; Shi, X.T. Fishway assessment and monitoring for endemic migratory fish using multiple techniques in high-altitude river systems: A case study from the Yarlung Zangbo River, Southeastern Tibetan Plateau. Glob. Ecol. Conserv. 2024, 56, e03325. [Google Scholar] [CrossRef]
- Nyqvist, D.; Nilsson, P.A.; Alenäs, I.; Elghagen, J.; Hebrand, M.; Karlsson, S.; Kläppe, S.; Calles, O. Upstream and downstream passage of migrating adult Atlantic salmon: Remedial measures improve passage performance at a hydropower dam. Ecol. Eng. 2017, 102, 331–343. [Google Scholar] [CrossRef]
- Bao, J.; Wang, X.; Li, W.; Zhang, C.; Mi, X.; Zhang, D.; Twardek, W.M.; Lin, H.; Qiao, Y.; Cooke, S.J.; et al. Passage efficiency and behavioral performance of Schizothorax davidi through different sections of a long vertical slot fishway. Water Biol. Secur. 2025, 4, 100330. [Google Scholar] [CrossRef]
- Ke, S.; Goerig, E.; Pang, K.; Ji, H.; Li, D.; Xu, J.; Tan, J.; Qi, H.; Shi, X. Evaluation of pool-and-weir fishway efficiency for the upstream spawning migration of Qinghai Lake’s naked carp. Ecol. Eng. 2024, 208, 107373. [Google Scholar] [CrossRef]
- Daneshfaraz, R.; Ghaderi, A.; Shahini, H.; Azali, A. Hydraulic performance assessment of Denil fishway with modified bed slope and baffle spacing. Results Eng. 2025, 26, 105072. [Google Scholar] [CrossRef]
- Gustafsson, S.; Österling, M.; Skurdal, J.; Schneider, L.D.; Calles, O. Macroinvertebrate colonization of a nature-like fishway: The effects of adding habitat heterogeneity. Ecol. Eng. 2013, 61, 345–353. [Google Scholar] [CrossRef]
- Shi, X.T.; Kynard, B.; Liu, D.F.; Qiao, Y.; Chen, Q.W. Development of Fish Passage in China. Fisheries 2015, 40, 161–169. [Google Scholar] [CrossRef]
- Zheng, T.; Tu, C.; Zhang, Z.; Sun, S.; Dai, H.; Li, G.; Liu, H. Vertical slot fishway design for fluctuating water-level reservoir. J. Hydraul. Res. 2025, 63, 117–125. [Google Scholar] [CrossRef]
- Li, S.S.; Sun, Z.Y.; Li, G.D.; Zhang, Z.X.; Wu, S.; Guo, L.H.; Liu, Z.J.; Zhang, M. Numerical investigation on the hydraulic characteristics and optimal design of pool-weir stepped fishway. Phys. Fluids 2025, 37, 062105. [Google Scholar] [CrossRef]
- Farzadkhoo, M.; Kingsford, R.T.; Suthers, I.M.; Felder, S. Flow hydrodynamics drive effective fish attraction behaviour into slotted fishway entrances. J. Hydrodyn. 2023, 35, 782–802. [Google Scholar] [CrossRef]
- Zheng, T.G.; Tu, C.Y.; Sun, S.K.; Huang, W.; Ren, W.C.; Li, G.N.; Liu, H.T. Testing Three Vertical Slot Fishway Configurations for a Chinese Endemic Fish. J. Hydraul. Eng. 2023, 149, 06023005. [Google Scholar] [CrossRef]
- Fuentes-Pérez, J.F.; Tuhtan, J.A.; Eckert, M.; Romao, F.; Ferreira, M.T.; Kruusmaa, M.; Branco, P. Hydraulics of Vertical-Slot Fishways: Nonuniform Profiles. J. Hydraul. Eng. 2019, 145, 06018020. [Google Scholar] [CrossRef]
- Marriner, B.A.; Baki, A.B.M.; Zhu, D.Z.; Cooke, S.J.; Katopodis, C. The hydraulics of a vertical slot fishway: A case study on the multi-species Vianney-Legendre fishway in Quebec, Canada. Ecol. Eng. 2016, 90, 190–202. [Google Scholar] [CrossRef]
- Fuentes-Pérez, J.F.; Sanz-Ronda, F.J.; Martínez, D.A.P.A.; García-Vega, A. Modeling Water-Depth Distribution in Vertical-Slot Fishways under Uniform and Nonuniform Scenarios. J. Hydraul. Eng. 2014, 140, 6014016. [Google Scholar] [CrossRef]
- Chen, M.; An, R.; Li, J.; Li, K.; Li, F. Identifying operation scenarios to optimize attraction flow near fishway entrances for endemic fishes on the Tibetan Plateau of China to match their swimming characteristics: A case study. Sci. Total Environ. 2019, 693, 133615. [Google Scholar] [CrossRef] [PubMed]
- Li, P.; Zhang, W.; Burnett, N.J.; Zhu, D.Z.; Casselman, M.; Hinch, S.G. Evaluating Dam Water Release Strategies for Migrating Adult Salmon Using Computational Fluid Dynamic Modeling and Biotelemetry. Water Resour. Res. 2021, 57, e2020WR028981. [Google Scholar] [CrossRef]
- Goettel, M.T.; Atkinson, J.F.; Bennett, S.J. Behavior of western blacknose dace in a turbulence modified flow field. Ecol. Eng. 2015, 74, 230–240. [Google Scholar] [CrossRef]
- Tarrade, L.; Texier, A.; David, L.; Larinier, M. Topologies and measurements of turbulent flow in vertical slot fishways. Hydrobiologia 2008, 609, 177–188. [Google Scholar] [CrossRef]
- Wu, S.; Rajaratnam, N.; Katopodis, C. Structure of Flow in Vertical Slot Fishway. J. Hydraul. Eng. 1999, 125, 351–360. [Google Scholar] [CrossRef]
- Bice, C.M.; Zampatti, B.P.; Mallen-Cooper, M. Paired hydraulically distinct vertical-slot fishways provide complementary fish passage at an estuarine barrier. Ecol. Eng. 2017, 98, 246–256. [Google Scholar] [CrossRef]
- Zheng, T.; Niu, Z.; Sun, S.; Huang, W.; Tu, C.; Liu, H.; Li, G.; Wang, H. Optimizing fish-friendly flow pattern in vertical slot fishway based on fish swimming capability validation. Ecol. Eng. 2022, 185, 106796. [Google Scholar] [CrossRef]
- Yan, J.F.; Chu, W.H.; Cao, Y.; Zhou, Q.L. Hydrodynamic analysis of fish swimming behavior in turbulent river confluences. Phys. Fluids 2024, 36, 121904. [Google Scholar] [CrossRef]
- Zhou, J.; Seo, J.H.; Mittal, R. Effect of hydrodynamic wakes in dynamical models of large-scale fish schools. Phys. Fluids 2025, 37, 011912. [Google Scholar] [CrossRef]
- Miao, J.; WANG, X.; XIN, P.; TANG, Z. Hydraulic characteristics of vertical slot fishway and allowable maximum water depth difference between inlet and outlet subject to insufficient inlet water depth. J. Hohai Univ. (Nat. Sci.) 2023, 51, 99–105. [Google Scholar] [CrossRef]
- Bravo-Córdoba, F.J.; Sanz-Ronda, F.J.; Ruiz-Legazpi, J.; Fernandes Celestino, L.; Makrakis, S. Fishway with two entrance branches: Understanding its performance for potamodromous Mediterranean barbels. Fish. Manag. Ecol. 2018, 25, 12–21. [Google Scholar] [CrossRef]
- O’ Connor, J.; Jones, M.; Amtstaetter, F.; Cornell, G.; Danger, A.; Ewing, T.; Fanson, B.; Stuart, I. Remediating a fishway entrance to improve fish attraction: A framework for success. J. Ecohydraulics 2025, 10, 1–11. [Google Scholar] [CrossRef]
- Xiao, L.; Wang, J.; Wang, B.; Jiang, H. China’s Hydropower Resources and Development. Sustainability 2023, 15, 3940. [Google Scholar] [CrossRef]
- Lyu, L.; Hu, J.; Feng, C.; Yin, Z. Influencing factors of fish passage in fishway of Duobu Hydropower Station in Xizang. Chin. J. Ecol. 2025, 44, 2678–2688. (In Chinese) [Google Scholar] [CrossRef]
- Zeng, S.C.; Tan, J.J.; Sun, J.J.; Wang, Y.Y.; Kattel, G.R.; Shi, X.T. Identifying the optimal flow conditions of a fishway with two entrances for endemic fishes at a high-altitude hydropower station in the Tibetan Himalaya, China. Ecol. Eng. 2025, 219, 107698. [Google Scholar] [CrossRef]
- Ke, S.; Yang, S.; Tu, Z.; Soomro, S.; Ji, H.; Li, D.; Xu, J.; Qi, H.; Shi, X. Swimming performance of a threatened native fish (Gymnocypris przewalskii) informs fishway design in Qinghai Lake. Hydrobiologia 2025, 852, 3997–4012. [Google Scholar] [CrossRef]
- Rodríguez, T.T.; Agudo, J.P.; Mosquera, L.P.; Gonzalez, E.P. Evaluating vertical-slot fishway designs in terms of fish swimming capabilities. Ecol. Eng. 2006, 27, 37–48. [Google Scholar] [CrossRef]
- Chen, K.; Tao, J.; Chang, Z.; Cao, X.; Ge, H. Difficulties and prospects of fishways in China: An overview of the construction status and operation practice since 2000. Ecol. Eng. 2014, 70, 82–91. [Google Scholar] [CrossRef]
- Fang, X.; Kumahor, S.; Tachie, M.F.; Katopodis, C.; Ghamry, H. Comprehensive Flow Turbulence Metrics to Improve Bar Rack Guidance for Downstream Migrating Fish. Water Resour. Res. 2024, 60, e2023WR034900. [Google Scholar] [CrossRef]
- Liu, M.M.; Rajaratnam, N.; Zhu, D.Z. Mean flow and turbulence structure in vertical slot fishways. J. Hydraul. Eng. ASCE 2006, 132, 765–777. [Google Scholar] [CrossRef]
- Zhang, Y.F.; Ko, H.T.; Calicchia, M.A.; Ni, R.; Lauder, G.V. Collective movement of schooling fish reduces the costs of locomotion in turbulent conditions. PLoS Biol. 2024, 22, e3002501. [Google Scholar] [CrossRef] [PubMed]
- Romão, F.; Branco, P.; Quaresma, A.L.; Amaral, S.D.; Pinheiro, A.N. Effectiveness of a multi-slot vertical slot fishway versus a standard vertical slot fishway for potamodromous cyprinids. Hydrobiologia 2018, 816, 153–163. [Google Scholar] [CrossRef]
- Li, M.; An, R.; Chen, M.; Li, J. Evaluation of Volitional Swimming Behavior of Schizothorax prenanti Using an Open-Channel Flume with Spatially Heterogeneous Turbulent Flow. Animals 2022, 12, 752. [Google Scholar] [CrossRef] [PubMed]
- Li, G.N.; Sun, S.K.; Zhang, C.; Liu, H.T.; Zheng, T.G. Evaluation of flow patterns in vertical slot fishways with different slot positions based on a comparison passage experiment for juvenile grass carp. Ecol. Eng. 2019, 133, 148–159. [Google Scholar] [CrossRef]
- Silva, A.T.; Katopodis, C.; Santos, J.M.; Ferreira, M.T.; Pinheiro, A.N. Cyprinid swimming behaviour in response to turbulent flow. Ecol. Eng. 2012, 44, 314–328. [Google Scholar] [CrossRef]
- Tan, J.J.; Tan, H.L.; Goerig, E.; Ke, S.F.; Huang, H.Z.; Liu, Z.X.; Shi, X.T. Optimization of fishway attraction flow based on endemic fish swimming performance and hydraulics. Ecol. Eng. 2021, 170, 106332. [Google Scholar] [CrossRef]
- Gilja, G.; Ocvirk, E.; Fliszar, R. Experimental Investigation of the Reynolds Shear Stress Exceedance Rate for the Injury and Disorientation Biocriteria Boundary in the Pool-Orifice and Vertical Slot Type Fishways. Appl. Sci. 2021, 11, 7708. [Google Scholar] [CrossRef]
- Baudoin, J.M.; Burgun, V.; Chanseau, M.; Larinier, M.; Ovidio, M.; Sremski, W.; Steinbach, P.; Voegtle, B. The ICE Protocol for Ecological Continuity—Assessing the Passage of Obstacles by Fish; Concepts, Design and Application; Onema: Vincennes, France, 2014. [Google Scholar]
- DVWK; Fisheries and Aquaculture Management Division. Fish Passes: Design, Dimensions and Monitoring; FAO/DVWK: Rome, Italy, 2002; ISBN 978-92-5-104894-8. [Google Scholar]
- Lu, Y.; Wang, Z.; Zhao, Z.; Zhao, D.; Zhang, Y. Hydraulic Characteristics of a New Vertical Slot Fishway with Staggered Baffles Configuration. Water 2025, 17, 809. [Google Scholar] [CrossRef]
- Du, Z.F.; Li, J.Q. VOF method in two-stage fourth order time-stepping framework. J. Comput. Phys. 2024, 496, 112580. [Google Scholar] [CrossRef]
- Flow Science. FLOW-3D User’s Manual; Version 10.2; Flow Science, Inc.: Santa Fe, NM, USA, 2013. [Google Scholar]
- Shahabi, M.; Ahadiyan, J.; Ghomeshi, M.; Narimousa, M.; Katopodis, C.; Azizi Nadian, H. Numerical study of the effect of a V-shaped weir on turbulence characteristics and velocity in V-weir fishways. River Res. Appl. 2023, 39, 21–34. [Google Scholar] [CrossRef]
- Yakhot, V.; Orszag, S.A. Renormalization group analysis of turbulence. I. Basic. Theory. J. Sci. Comput. 1986, 1, 3–51. [Google Scholar] [CrossRef] [PubMed]
- Tan, J.J.; Gao, Z.; Dai, H.C.; Yang, Z.Y.; Shi, X.T. Effects of turbulence and velocity on the movement behaviour of bighead carp (Hypophthalmichthys nobilis) in an experimental vertical slot fishway. Ecol. Eng. 2019, 127, 363–374. [Google Scholar] [CrossRef]
- Vassilicos, J.C. Dissipation in Turbulent Flows. Annu. Rev. Fluid Mech. 2015, 47, 95–114. [Google Scholar] [CrossRef]
- Mao, X. Review of fishway research in China. Ecol. Eng. 2018, 115, 91–95. [Google Scholar] [CrossRef]
- Puertas, J.; Cea, L.; Bermúdez, M.; Pena, L.; Rodríguez, Á.; Rabuñal, J.R.; Balairón, L.; Lara, Á.; Aramburu, E. Computer ap-plication for the analysis and design of vertical slot fishways in accordance with the requirements of the target species. Ecol. Eng. 2012, 48, 51–60. [Google Scholar] [CrossRef]
- Chen, A.; Wu, M.; Chen, K.; Sun, Z.; Shen, C.; Wang, P. Main issues in research and practice of environmental protection for water conservancy and hydropower projects in China. Water Sci. Eng. 2016, 9, 312–323. [Google Scholar] [CrossRef]
- Ma, B.; Dong, F.; Peng, W.Q.; Liu, X.B.; Huang, A.P.; Chen, X.K.; Hou, L.; Wang, W.J.; Si, Y.; Yao, J.W. Numerical simulation of effects of inlet water depth of ecological fishway on the suitability of passing fish. IOP Conf. Ser. Earth Environ. Sci. 2019, 344, 12064. [Google Scholar] [CrossRef]
- Yuan, H.; Chen, B.; Sun, Q.; Xie, C.; He, X. Deciphering the effect of variation in slope on flow characteristics in a vertical slot fishway. J. Hydro-Environ. Res. 2024, 54, 1–12. [Google Scholar] [CrossRef]
- Mulligan, K.B.; Haro, A.; Towler, B.; Sojkowski, B.; Noreika, J. Fishway Entrance Gate Experiments with Adult American Shad. Water Resour. Res. 2019, 55, 10839–10855. [Google Scholar] [CrossRef]
- Shen, C.; Yang, R.; Wang, M.; He, S.; Qing, S. Application of Vortex Identification Methods in Vertical Slit Fishways. Water 2023, 15, 2053. [Google Scholar] [CrossRef]
- Calluaud, D.; Pineau, G.; Texier, A.; David, L. Modification of vertical slot fishway flow with a supplementary cylinder. J. Hydraul. Res. 2014, 52, 614–629. [Google Scholar] [CrossRef]
- Ouyang, L.; Li, D.; Cui, S.; Wu, X.; Liu, Y.; Han, X.; Zhou, S.; Xu, G.; Tu, X.; Chen, K.; et al. Fish Swimming Behavior and Strategies Under Different Hydrodynamic Conditions in Fishways with Various Vertical Slot Configurations. Fishes 2025, 10, 415. [Google Scholar] [CrossRef]
- Quaresma, A.L.; Romão, F.; Branco, P.; Ferreira, M.T.; Pinheiro, A.N. Multi slot versus single slot pool-type fishways: A modelling approach to compare hydrodynamics. Ecol. Eng. 2018, 122, 197–206. [Google Scholar] [CrossRef]
- Cai, L.; Hou, Y.; Katopodis, C.; He, D.; Johnson, D.; Zhang, P. Rheotaxis and swimming performance of Perch-barbel (Percocypris pingi, Tchang, 1930) and application to design of fishway entrances. Ecol. Eng. 2019, 132, 102–108. [Google Scholar] [CrossRef]
- Chen, X.F.; Liu, S.K.; Wang, Y.M.; Hao, Y.T.; Li, K.F.; Wang, H.T.; Liang, R.F. Restoration of a fish-attracting flow field downstream of a dam based on the swimming ability of endemic fishes: A case study in the upper Yangtze River basin. J. Environ. Manag. 2023, 345, 118694. [Google Scholar] [CrossRef]
- Elings, J.; Bruneel, S.; Pauwels, I.S.; Schneider, M.; Kopecki, I.; Coeck, J.; Mawer, R.; Goethals, P.L.M. Finding navigation cues near fishways. Biol. Rev. 2024, 99, 313–327. [Google Scholar] [CrossRef]














| Working Condition | Distance Between the Two Entrances | Water Depth of Exit | Water Depth of 1# Entrance | Water Depth of 2# Entrance |
|---|---|---|---|---|
| W1 | NA | 2.5 m | 2.3 m | NA |
| W2 | NA | 2.5 m | 2.0 m | NA |
| W3 | NA | 2.5 m | 1.8 m | NA |
| W4 | 100 m | 2.5 m | 2.0 m | 1.8 m |
| W5 | 100 m | 2.5 m | 1.8 m | 1.6 m |
| W6 | 200 m | 2.5 m | 1.8 m | 1.5 m |
| W7 | 200 m | 2.5 m | 1.7 m | 1.4 m |
| W8 | 200 m | 2.5 m | 1.6 m | 1.3 m |
| W9 | 300 m | 2.5 m | 1.6 m | 1.2 m |
| W10 | 300 m | 2.5 m | 1.5 m | 1.1 m |
| Species of Fish | Induced Swimming Speed | Critical Swimming Speed | Brust Swimming Speed |
|---|---|---|---|
| Mean (Range)/(BL/s) | Mean (Range)/(BL/s) | Mean (Range)/(BL/s) | |
| Schizothorax oconnori | 0.81 (0.69–0.87) | 4.13 (3.41–4.25) | 5.63 (4.17–6.67) |
| Schizothorax macropogon | 0.35 (0.26–0.40) | 3.56 (2.85–3.93) | 4.87 (4.31–5.47) |
| Schizothorax waltoni | 0.98 (0.84–1.05) | 3.31 (2.95–3.45) | 4.77 (3.60–5.12) |
| Mesh | Cell Size | Number of Cells | Mesh Resolution |
|---|---|---|---|
| M1 | 0.08 m | 6,135,676 | Fine |
| M2 | 0.12 m | 3,154,628 | Medium |
| M3 | 0.16 m | 1,952,352 | Coarse |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Share and Cite
Huang, X.; Tan, J.; Wang, Y.; Sun, J.; Zeng, S.; Wu, S.; Shi, X. Hydraulic Characteristics and the Adaptability to Water-Level Fluctuation of the Vertical-Slot Fishway. Water 2026, 18, 432. https://doi.org/10.3390/w18030432
Huang X, Tan J, Wang Y, Sun J, Zeng S, Wu S, Shi X. Hydraulic Characteristics and the Adaptability to Water-Level Fluctuation of the Vertical-Slot Fishway. Water. 2026; 18(3):432. https://doi.org/10.3390/w18030432
Chicago/Turabian StyleHuang, Xianglong, Junjun Tan, Yuanyang Wang, Junjian Sun, Sicheng Zeng, Shuaijie Wu, and Xiaotao Shi. 2026. "Hydraulic Characteristics and the Adaptability to Water-Level Fluctuation of the Vertical-Slot Fishway" Water 18, no. 3: 432. https://doi.org/10.3390/w18030432
APA StyleHuang, X., Tan, J., Wang, Y., Sun, J., Zeng, S., Wu, S., & Shi, X. (2026). Hydraulic Characteristics and the Adaptability to Water-Level Fluctuation of the Vertical-Slot Fishway. Water, 18(3), 432. https://doi.org/10.3390/w18030432

