4.1. Beach Litter Abundance and Composition
In each season, a total amount of 9236 m
2 of beach surface was surveyed at the selected sites, and 5627 macro beach litter items were collected (
Table 2): 509 items at Goro (0.71 items/m
2); 2258 items at Volano (0.94 items/m
2); 495 items at Estensi (0.47 items/m
2); 2365 items at Spina (0.47 items/m
2). Based on the average number of items collected across the four seasonal surveys at each site, the CCI application enabled the classification of all sites as “Clean” (Goro, CCI = 3.56; Volano, CCI = 4.69; Estensi, CCI = 2.33; Spina, CCI = 2.34).
The greatest abundance of litter (
Table 2) was observed at Volano in spring 2024 (0.47 items/m
2) and the lowest at Spina in summer 2024 (0.07 items/m
2), despite an increase in beach users during the survey period. The results obtained at Volano are consistent with previous studies conducted at the same site in spring 2015, which reported a litter density of 0.57 items/m
2 [
86]. Studies conducted along the Spanish coast and the Moroccan Mediterranean coast demonstrated a correlation between tourist influx and an increase in beach litter [
48,
87,
88,
89,
90]. However, the results of the summer survey at the Spina site suggest that the diminished presence of litter during the summer is primarily due to beach-cleaning initiatives, which are typically implemented from June to September. Other authors conducting comparative studies across multiple beaches in the Adriatic-Ionian macro-region have reported similar findings [
47]; these authors observed the lowest litter densities on beaches in Bosnia-Herzegovina, which are regularly cleaned by adjacent hotel resorts, compared to other surveyed sites (Albania, Croatia, Greece, Italy, Montenegro, and Slovenia). The Spina site is located in front of a four-star campsite, suggesting that beach cleaning operations were likely conducted with greater attention during the summer.
The average litter density observed in this study (0.61 items/m
2 ± 0.16 SD) (
Table 2) is higher than the values reported in other studies conducted on the Northern Italian Adriatic coast (from 0.30 to 0.35 items/m
2) [
47,
91]. The beach litter density in the Mediterranean Sea varies considerably: 0.2 items/m
2 were found along the Albanian Adriatic and Ionian coastlines [
92,
93], 0.05–0.06 items/m
2 were recorded for the Moroccan and Greek Mediterranean regions [
87,
94], and 0.12 items/m
2 were found along the Alicante Spanish coast [
48].
Table 3 summarizes the average litter densities recorded in this study and values reported in other investigations conducted in the Mediterranean Sea. The mean litter abundance collected along the Istanbul coast (Marmara Sea, Turkey) was 5.50 ± 4.46 items/m
2 [
95], which is much higher than the values reported in this paper. A recent citizen science study across 12,000 km of the eastern Pacific coast of Latin America (Mexico, Guatemala, El Salvador, Costa Rica, Panama, Colombia, Ecuador, Peru, and Chile) reported densities ranging from 0.46 to 2.26 items/m
2 [
96]. The observed data suggest that the beach litter abundance depends on a combination of local environmental factors (e.g., sea state and coastal morphology) and anthropogenic factors (e.g., human behavior and waste management strategies) [
97,
98,
99,
100,
101,
102,
103,
104].
According to the “Macro Marine Litter Master List” [
23,
47,
76], the beach litter composition collected over the course of a year can be referred to as belonging to one of eight macro categories of materials: artificial polymer materials, rubber, cloth/textile, paper/cardboard, processed/worked wood, metal, glass/ceramics, unidentified materials and/or chemicals. These macro categories correspond to 112 item categories. On average, 39 different categories were recorded per beach, indicating a relatively high degree of diversity.
Plastic accounted for 93.89% of all items collected across the four sampling sites. Other major material categories (glass/ceramics, textiles, rubber, paper, processed wood, metal, and unidentified/chemical substances) were minimally represented (<4%,
Figure 2e). This pattern is consistent with previous studies conducted in the Adriatic Sea, the Mediterranean Basin, and other locations worldwide [
47,
81,
87,
114,
115,
116,
117,
118,
119,
120]. Plastics are estimated to comprise 60–80% of all marine debris, reaching or exceeding 95% in certain regions [
35,
36]. For example, plastics have been reported to comprise over 94% of total debris along the Croatian coast [
108] and along the beaches of Central Vietnam [
121], further emphasizing the ubiquity and persistence of plastic pollution in diverse coastal environments.
The litter composition varied from site to site, with substantial differences in material types (
Figure 2). One exception of particular relevance was recorded at the Goro site in autumn 2023, when glass fragments accounted for 42% of the total litter, probably due to the recent breakage of one or more glass objects.
Based on the “Joint List of Litter Categories for Marine Macrolitter Monitoring” [
83], a list of the “top 10” collected items was compiled, considering all surveys and sites. The list included 2.5–50 cm plastic pieces (J79, 18%); fragments of plastic bags (J5, 12%); cotton bud sticks (J95, 12%); 2.5–50 cm polystyrene pieces (J82, 6%); mussel nets (J45, 4%); 0–2.5 cm plastic pieces (J9903, 4%); food containers (J225, 3%); 2.5–50 cm plastic/polystyrene pieces (J224, 3%); plastic drink lids (J21, 3%); cigarette butts and filters (J27, 3%). The composition of the top 10 items varied across the four surveyed sites, as illustrated in
Figure 3, and coincided with observations reported in similar studies [
37,
47,
48,
81,
114,
119,
120,
122,
123].
The top 10 items included undefined use (NN, 59%), personal hygiene and care-related (HY, 18%), food consumption-related (FC, 13%), aquaculture-related (AQ, 6%), and smoking-related (SM, 4%). Notably, all of the top 10 items observed at Goro are composed of plastic materials.
Figure 4 shows a bar chart that considers all collected items and provides a clear visual comparison of how different usage categories contribute to the overall litter composition.
The undefined use category comprises the largest proportion of litter (NN, 55%). These items include plastic pieces, fragments of plastic bags, and polystyrene pieces, which, in accordance with other studies [
28,
124,
125], are among the most abundant and are often the result of larger objects’ fragmentation. Identifying the sources of environmental release and implementing effective management strategies for this group of items remains a complex challenge [
47,
90]. Food consumption-related items (FC, 15%) also constitute a substantial portion of the total debris. This group includes single-use items such as food containers, plastic and polystyrene pieces, and plastic drink lids. Many of these items are widely produced and used [
126] and likely originate from land-based sources, being transported via rivers to the coastal environment. Additionally, poor waste disposal practices by beach users contribute to their frequent occurrence along shorelines [
94]. Food-related single-use items account for approximately 36% of global plastic production [
127]. Although this study’s classification was based on usage categories rather than source attribution, it is important to note that the determination of the land-based or marine-based origin of beach litter is challenging [
82]. The source of litter items is often uncertain due to the overlapping pathways through which debris reaches coastal environments. Only debris directly associated with fishing and aquaculture activities can be confidently assigned to a marine origin, and the origin of other items remains ambiguous. For example, commonly collected items, such as cigarette butts (J27) or sweet wrappers (J30), may come from direct deposition by beach users or from improper waste disposal at sea by passengers on vessels. Nevertheless, categorizing by usage type allows for an analysis of potential release pathways and provides useful information to support targeted management strategies, aligning with the recommendations of the “Joint List of Litter Categories for Marine Macrolitter Monitoring” [
83]. Another notably represented category at the investigated sites is personal hygiene- and care-related litter (HY, 13%). Cotton bud sticks made of plastic materials accounted for 94% of the items in this category, despite the fact that their production and sale in non-biodegradable form has been banned in Italy since 1 January 2019 (Italian Law 232/2016), aligning with EU Directive 2019/904, which aims to reduce single-use plastic products. The presence of significant quantities of these items may be attributed to legacy pollution, i.e., they were released and accumulated prior to the national ban’s enforcement. Previous studies [
82,
128,
129] suggest that personal hygiene-related items often enter coastal and marine environments through improper disposal in domestic sewage systems and sewage overflows. Inefficient wastewater treatment is a major pathway for their release [
28,
91,
130]. The prevalence of litter associated with improper sewage disposal also raises concerns about the potential co-occurrence of fecal coliforms and their implications for human health [
123,
131]. Despite the widespread presence in the area of fishing and aquaculture activities, related items were poorly represented (4% each). These values are lower than those previously reported for the Adriatic-Ionian macro-region, where fishing and aquaculture debris accounted for approximately 14%, especially along Italian coasts [
47]. These findings are encouraging and may reflect the outcomes of transnational initiatives implemented in recent years, including the stakeholder engagement projects and awareness campaigns aimed at reducing marine litter from fishing and aquaculture activities. The remaining use-related categories (SM, HU, CO, MD, RE, AG, and CL) were marginally represented (<4%), though some seasonal increases were observed and will be discussed later.
4.2. Seasonal Variation
The amount of litter varied from place to place and according to the season (
Figure 5). At three of the four sites investigated (Goro, Volano, and Spina), the percentage of litter collected in the summer was similar, representing approximately 15% of the total amount collected throughout the year. At Goro, 50% of the total litter was collected in autumn 2023, while only 12% was collected in winter 2024. At Volano, 50% of the litter was collected in spring 2024. Overall, the highest abundance of litter was observed in spring (
Figure 5). A substantial reduction in mean contamination levels occurred during summer, followed by an increase throughout autumn and winter. This trend is primarily due to periodic beach cleaning activities, including mechanical cleaning by local municipalities and manual cleaning by volunteers, which occur more frequently between June and September.
The density of litter items was calculated for each sampling site and seasonal survey, and the CCI was applied to evaluate the overall cleanliness status of the beach (
Table 2). The Goro site was classified as “Clean” during summer 2023 and spring 2024 (CCI = 2.18 and 3.30, respectively), “Moderately clean” in autumn 2023 (CCI = 7.11), and “Very clean” in winter 2024 (CCI = 1.65). The Volano site was classified as “Clean” during summer and autumn 2023 and winter 2024 (CCI ranged from 2.68 to 3.64), but was “Moderately clean” in spring 2024 (CCI = 9.43). A previous study conducted at Volano in spring 2015 classified it as “Dirty” (CCI = 11.4) [
86]. The Estensi site was predominantly classified as “Clean” in summer and autumn 2023 (CCI = 2.17 and 2.19, respectively) and in spring 2024 (CCI = 3.11), but it was classified as “Very clean” in winter 2024 (CCI = 1.87). The Spina site was classified as “Clean” during the samplings carried out in autumn 2023 and winter and spring 2024 (i.e., CCI = 2.47 to 2.88), but it was classified as “Very clean” in summer 2024 (CCI = 1.43). As observed by different authors, the CCI varies significantly both spatially and temporally. Previous investigations at Boccasette Beach, located on the northern coastline of the Po Delta in Italy [
91], classified the beach as “Moderately clean” in November and December 2019; the same beach, sampled in February and June 2020, was classified as “Clean”, but it reverted to “Moderately clean” in October 2020. Along the Istanbul coast (Marmara Sea, Turkey), four beaches were predominantly classified as “Dirty” or “Extremely dirty”, with lower CCI values observed during autumn and winter [
95]. An 18-year assessment of the effectiveness of Israel’s “Clean Coast” program showed that the average CCI value was higher in winter (i.e., 4.8) than in other seasons (CCI = 4.6) [
132]. Surveys of fourteen Mediterranean beaches in Morocco revealed a minimal average difference in CCI between autumn and spring (1.00 ± 0.68 and 0.86 ± 0.73, respectively), enabling the classification of 87.5% of the beaches as “Very clean” and 12.5% as “Clean” [
87]. However, it is challenging to make seasonal comparisons with other CCI studies due to the frequent reporting of only average values across each study area without precise indications of their seasonal variations.
Previous studies [
25,
133] have shown that environmental conditions play a significant role in the redistribution of litter. Once introduced into the marine environment, litter accumulates along the shoreline, carried by wave action and wind [
52]. This is particularly evident from October to May, when beach users are scarce and manual and mechanical cleaning activities are reduced or absent. During these months, environmental factors primarily govern the distribution of beach litter. The surface cyclonic circulation of the Adriatic Sea significantly influences the transport and distribution of marine litter along the Italian coastline [
134]. Sampling activities were carried out under various wave conditions, and maximum wave height values ranged between 0.10 and 1.75 m (
Figure 6). The yearly mean wave height was 0.65 m, with maximum average values ranging from 2.54 m in summer to 4.08 m in spring. As illustrated in
Figure 6, the most energetic event occurred around mid-April, producing the highest recorded wave.
According to a public notice issued on 24 July 2024 by the Po River Basin Authority (
https://www.adbpo.it/fiume-po-la-disponibilita-idrica-e-ai-massimi-storici/, accessed on 30 June 2025), the highest average monthly discharge values were recorded at the Pontelagoscuro monitoring station (Ferrara) in March and June of 2024 (3174 and 2926 m
3/s, respectively). Near-peak values were also observed in April and May and are likely primarily attributed to seasonal snowmelt. Due to the significant inflows from the Po River basin during this period, it is reasonable to conclude that increased amounts of litter were carried by rivers into the marine environment and subsequently dispersed along the coast by wind and waves.
Compared to the general pattern observed across all sites and surveys, seasonal variations reveal few differences in the composition or use of the items most frequently collected (
Figure 7).
During the summer, a relative increase in smoking-related debris (SM, 7%) and fisheries-related litter (FI, 4%) was observed alongside a proportional decrease in items classified as having an undefined use (NN, 42%). These findings suggest that direct human activities, particularly recreational beach use and coastal fishing, have a greater influence on the composition of summer litter (
Figure 7a). As previously mentioned, a distinct departure from the typical plastic-only composition was recorded in autumn due to the presence of glass fragments at Goro (
Figure 7b). The presence of personal hygiene-related items (HY, 16%) increased, as did hunting-related debris (HU, 3%), likely due to land-based seasonal activities. Plastic items became predominant again during the winter (
Figure 7c), with a proportional increase in personal hygiene products (HY, 14%) and food consumption-related items (FC, 11%). Due to the low beach attendance during this season, the collected litter likely originated from land-based sources. It was then transported to the coast by riverine processes and stranded on the beach by marine currents. This reflects the importance of wastewater discharges and overflows as sources of litter. An increase in plastic bag fragments (J5, 20%) was observed during the spring (
Figure 7d) and originated from seasonal riverine inputs or the redistribution of previously accumulated litter along the shoreline. Wave data recorded during the spring period support the latter hypothesis, as the highest mean wave height (4.08 m) was observed during this time. These high-energy wave conditions likely promoted the resuspension and transport of debris toward the monitored sites, contributing to the accumulation of lightweight and easily transportable items, such as plastic bag fragments. The distribution of use-related categories remained consistent with the trends observed in other seasons. The undefined use category (NN) predominated at 46%, followed by personal hygiene (HY) and food consumption (FC) items at 10% each. These seasonal patterns highlight the diversity of sources and transport mechanisms of beach litter and reflect the interplay between human activities and environmental processes throughout the year.
The presence of smoking-related items, specifically cigarette butts and filters (J27), has been widely documented as one of the most common forms of beach litter pollution on a global scale [
37,
48,
49,
122,
135,
136,
137,
138,
139]. Previous studies in the Emilia-Romagna and Veneto Regions identified cigarette butts as the most prevalent item (22.9%) [
86]. Similar studies on the German and Lithuanian coasts reported values ranging from 12% to 19%, depending on visitor density and beach maintenance programs [
140]. In contrast, a Portuguese site with few beachgoers and no clean-up initiatives showed a negligible presence of such items (0.1%) [
141]. Several authors have proposed that the prevalence of cigarette butts may serve as an indicator of beach management efficiency, environmental awareness, and recreational pollution [
142,
143,
144]. In this study, a clear summer peak was observed (J27, 7%), but smoking-related items were surprisingly limited overall, possibly reflecting growing public awareness and successful local mitigation efforts.
Regarding fishing-related debris, which was primarily detected during the summer, it is notable that the summer sampling occurred in July, before the professional fishing closure in August to restore the natural habitat. Despite the prevalence of fishing activity in the study area, only 3% of the collected items were fishing-related, which may seem anomalous. However, similar low proportions have been observed along the Iranian coast of the Strait of Hormuz, where fishing debris comprised only 2% of marine litter despite intensive local fishing activity [
145]. Similarly, on São Vicente island (Cape Verde), where fisheries are a mainstay of the local economy, fishing-related litter constituted only 13–30% of beach debris [
119].
The presence of shotgun cartridges (J70) detected in autumn is likely associated with the start of the 2023–2024 hunting season in Emilia-Romagna, which spanned from mid-September to late January. The study area is located in the Po Delta, a Ramsar-listed wetland and popular waterfowl hunting destination. Therefore, it is reasonable to attribute these items to hunting activities conducted in back-dune areas or nearby coastal zones. Seasonal variation in cartridge abundance has also been noted on the Danish beaches, with lower values from July to December and higher values in the first half of the year [
146]. Shotgun cartridges, which are typically associated with coastal game hunting, were consistently recorded across multiple surveys on a Portuguese beach, where they were among the most common types of litter [
141].
The Kruskal-Wallis test was employed to assess the variability of litter abundance across the four sites. It revealed a significant pattern at the Goro site (
p < 0.05). When comparing litter abundance within each site across different seasons, significant variations were observed at Goro and Volano (
p < 0.005 and
p < 0.01, respectively). Estensi and Spina showed no significant seasonal differences (
p > 0.5).
Figure 5 shows outliers in the seasonal litter distribution. The non-metric multidimensional scaling analysis (nMDS) revealed clear groupings by season and site based on litter abundance (expressed as items/m
2). The orientation of the vectors in the nMDS plot (
Figure 8a) reflects the distribution of the most characteristic litter items for each site. Greater distances between points on the plot reflect greater differences in litter composition. All differences/similarities are also apparent in the cluster analysis (
Figure 8b). Although the analysis was performed on the entire dataset, for illustrative purposes, the most pronounced seasonal differences observed between sites are highlighted below, referring only to the top 10 items per site and season. For example, the Spina site exhibited similar trends in autumn, winter, and spring, as reflected by the close proximity of the respective points in
Figure 8. The summer evaluation appears distinct and reflects a notable divergence from the group. Items that contributed to this distinction include cigarette butts and filters (J27), plastic rings from drink bottles (J24), and strings and cords less than 1 cm in diameter (J233). These items were present in the summer but absent in the other seasons. Additionally, cotton bud sticks (J95) were recorded at a density of 0.01 items/m
2 in summer, compared to an average of 0.07 items/m
2 across the other seasons. A similar trend was observed at Volano (
Figure 8), where the highest densities of plastic fragments measuring 0–2.5 cm (J9903, 0.03 items/m
2) were recorded in summer and were sparsely represented during the other seasons (mean value: 0.01 items/m
2). Cigarette butts (J27, 0.01 items/m
2) and freezer bags, including fragments (J4, 0.01 items/m
2), were recorded in summer but were rare or absent in the other seasons. The difference is even more pronounced at Estensi (
Figure 8), where the top 10 in the summer includes several categories not recorded in any other season: freezer bags and fragments (J4), strings and cords less than 1 cm in diameter (J233), non-food plastic lids (J22), and plastic cups and cup lids (J227). Summer also saw a relative increase in the abundance of small plastic fragments (0–2.5 cm, J9903) and cigarette butts (J27), which were poorly represented or absent in the other seasons. The Goro site works differently throughout the year, as reflected by the distances between the points in
Figure 8, which reflect variations in the litter categories, highlighting a high degree of temporal variability in litter composition. Notably, the litter items represented in
Figure 8a belong to categories related to beach users (e.g., food containers—J225; sweet wrappers—J30) and fishing activities (e.g., strings and cords—J233). All of the litter items represented in the graph have positive buoyancy, which allows them to be easily transported by rivers, waves, and currents and to freely move among nearby beaches, as suggested by Asensio-Montesinos et al. [
147] and Ciufegni et al. [
90].
Figure 8a shows that drink bottles (J7 and J8) are characteristic of the autumn survey at Goro. Crisp packets and sweet wrappers (J30) are characteristic of spring and autumn at Goro. Plastic cups and lids (J227) are common at Volano in winter and spring and at Goro in spring. Strings and cords (J233) are common at Volano in autumn and summer and at Estensi in summer.
4.3. Cross-Shore Variation
Regarding the cross-shore distribution of litter, it should be noted that at the Goro site, only the SZ was identified. At the Volano, Estensi, and Spina sites, five geomorphological zones of interest (SZ, LB, UB, DF, and DC) were distinguished. In addition, a few observations must be made:
During autumn 2023 at the Volano site, the beach profile differed significantly from that observed in other seasons. Therefore, it was not possible to distinguish between the LB and UB zones. This condition was likely associated with the sea state, which exhibited wave peaks between 1.5 and 2.5 m during the days preceding the sampling (
Figure 6). All items from the backshore were allocated to the LB, and the UB data were reported as not available (NA). To determine the density of collected items (and related CCI) within the backshore, the total item count was normalized to the entire sampled area (by summing the area of the two zones).
During mechanical cleaning operations in the study area, it is a common practice to relocate large trunks and stranded vegetation to the base of the dune in order to clear the beach zones that are typically used by visitors, such as the SZ and the backshore. This procedure results in the relocation of sediments, beach litter, and organic debris at the foot of the dune. This contributes to a rise in the dune crest and an increase in the dune foot slope. In this context, during summer 2024, only a few litter items were collected at Spina along the DF, and the DC was inaccessible and could not be surveyed (NA).
In all other instances where no items were found in a given zone, the corresponding density and CCI values were reported as 0 (i.e., LB at Spina in winter 2024 and at Estensi in summer 2023; DC at Volano in summer 2023).
The average density and CCI calculated for each zone are summarized in
Table 4. A chromatic scale was applied to enable the quick identification of the five beach cleanliness categories: light blue for “Very clean”, green for “Clean”, yellow for “Moderately clean”, orange for “Dirty”, and red for “Extremely dirty”.
In summary (
Table 4), considering all the samples collected, it can be concluded that the SZ was generally classified as “Clean” (0.12 items/m
2, CCI = 2.25, 14.61%), the LB was classified as “Very clean” (0.08 items/m
2, CCI = 1.55, 10.52%), the UB was classified as “Moderately clean” (0.29 items/m
2, CCI = 5.75, 40.43%), the DF was classified as “Dirty” (0.51 items/m
2, CCI = 10.25, 24.45%), and the DC was classified was classified as “Clean” (0.10 items/m
2, CCI = 1.77, 9.99%). At Volano, Estensi, and Spina, and in cases where the different geomorphological zones could be distinguished, the calculated densities revealed a clear general cross-shore trend, with lower litter abundance in the SZ and LB, an increasing accumulation from the UB to the DF, and a decrease at the DC. However, the averaged litter density and CCI results tend to mask seasonal and site-specific patterns (
Appendix A).
Other authors who analyzed litter distribution along the sea-to-land gradient of Italian coasts in the Southern Adriatic and Ionian Seas have also observed similar trends; their findings indicate that beach litter predominantly accumulates in the supratidal zone (backshore) and at the dune foot rather than in the intertidal zone [
148,
149,
150]. Further investigations along the Spanish, Korean, and Japanese coasts generally indicate that most litter accumulates beyond the strandline [
49,
89,
130,
151,
152]. Other authors assert that the backshore zone is more contaminated than the intertidal zone [
153,
154,
155].
An interesting finding of the cross-shore CCI analysis is that the UB and DF zones determine overall beach cleanliness. The transport of beach litter, which is mainly driven by marine weather forcing, likely facilitates its accumulation in these areas. This suggests that targeted cleaning interventions, aimed at reducing the presence of items in these zones, could substantially improve beach cleanliness. Summer 2024 sampling at the Spina site supports this observation, revealing the lowest item density and the lowest CCI values recorded at the UB and DF zones (
Table 2,
Table A4). During the above survey, an accumulation of large trunks, branches, sediments, and debris was observed near the dune, which likely reduced the number of items collected in the other beach zones. However, to ensure that the presence of beach litter is addressed effectively, rather than merely masked, it would be more beneficial to conduct targeted manual collections of beach litter before relocating large trunks and other residues toward the dune. As demonstrated in prior studies, the presence of beach litter is frequently underestimated because commonly employed methodologies do not account for buried litter within sediments, which remains concealed until subsequent re-exposure [
98,
156,
157,
158]. Coastal erosion has been shown to unearth buried litter from dune systems and other coastal environments in both the short and long term [
159]. Another study observed that eroded dunes on the Portuguese Atlantic coast released a sand layer contaminated with marine debris that subsequently dispersed onto the beach [
160]. Moreover, overwash events appear to influence the accumulation and distribution of beach litter. This suggests a parallel trend between the fate of the litter and eroded sediments. These materials are redistributed within the coastal system through overwash, dune landslides, and flooding [
161]. Further investigation is needed to determine how much litter is trapped or concealed by common coastal management practices, such as relocating sediments, trunks, and debris at the dune foot. It is also necessary to ascertain whether these practices contribute to the reappearance of litter in the absence of mechanical cleaning or during adverse marine weather events. In the present study, all visible macro beach litter items were collected according to the guidelines of the selected sampling protocol [
23,
47,
75,
76]. When partially buried items were encountered, they were extracted from the sediment and included in the collection. However, it is important to acknowledge that commonly used monitoring methodologies primarily focus on surface debris and therefore tend to underestimate the actual quantity and potential environmental impact of litter in coastal environments. This concealed fraction of debris may persist in the sediment for extended periods, gradually degrading and releasing microplastics and other hazardous substances into the environment. Furthermore, buried litter complicates recovery efforts, hinders visual detection, and may mislead public perception of beach cleanliness. Future studies could develop and integrate complementary investigative methods, such as sediment sieving or core sampling, to provide a more thorough assessment of beach pollution.
Comparing the general litter composition across all sites and surveys to the patterns observed within each geomorphological zone reveals compositional and functional differences, reflecting the influence of distinct spatial sources and processes (
Figure 9).
The SZ exhibited a distinctive profile characterized by the inclusion of non-plastic materials (
Figure 9a), most notably glass fragments, which accounted for 22% of the top 10 collected items. This anomaly is primarily attributable to the autumnal survey at the Goro site. Additionally, 75% of the top 10 items in this zone fall into the undefined use category (NN), and the remaining 25% fall into the food consumption category (FC). This suggests a more direct influence from human activities, such as recreational beach use. The LB’s composition closely aligns with patterns typically observed across the full dataset. All of the top 10 items in the LB are made of plastic materials (
Figure 9b). However, the LB showed slightly reduced dominance of items in the undefined use category (NN, 44%) and an increased presence of items in the personal hygiene (HY, 12%), aquaculture (AQ, 3%), and smoking-related (SM, 3%) categories (
Figure 9b). This composition suggests a mixed input from both marine and terrestrial sources, reflecting the UB’s transitional position within the coastal system. The UB presented a further shift, with a notable increase in personal hygiene-related items (HY, 14%) and hunting-related debris (HU), such as shotgun cartridges, which accounted for 3% of the most collected items (
Figure 9c). The undefined use category remained relevant but was comparatively lower (NN, 39%), indicating the contribution of diverse sources, likely from inland and seasonal activities. The presence of aquaculture-related items (AQ, 5%) further highlights the complexity of litter pathways in this zone. In the DF, personal hygiene items (HY, 17%) and aquaculture-related items (AQ, 7%) predominated, with items of undefined use accounting for 38% of the total (NN). The presence of shotgun cartridges (J70, 3%;
Figure 9d) was consistent with that observed in the UB, suggesting the influence of similar inland sources. While the overall composition consisted of plastic material, the balanced representation across usage categories indicates a broader spectrum of litter inputs. The DC was characterized by a predominance of lightweight plastic items (
Figure 9e), including a notably high percentage of plastic bag fragments (J5, 19%) and polystyrene pieces (J82, 13%). The undefined use category was highest in this zone (NN, 48%), though substantial amounts of food-related (FC, 11%), personal hygiene (HY, 8%), and aquaculture-related (AQ, 6%) debris were also present. The exclusive presence of plastic materials and the overrepresentation of items easily transported by wind imply that this zone acts as a long-term sink, capturing debris redistributed from lower beach areas. The cross-shore distribution of litter items highlights the dynamic and compartmentalized nature of beach litter accumulation, which is influenced by human activities, environmental transport mechanisms, and the spatial structure of the beach-dune system.
Consistent with findings reported in Korea [
152], Japan [
151], Spain [
49], and along the North African coasts of the Strait of Gibraltar [
88], most of the litter observed in this study was concentrated in the upper areas of the beach (UB and DF). Similarly, a monitoring campaign conducted in the Tuscany Region (Italy) using UAV technology revealed high concentrations of debris near the dune foot [
162]. Numerous studies have documented variations in beach litter accumulation according to coastal geomorphology, elevation, or the presence of vegetation [
154,
163,
164,
165,
166,
167,
168,
169]. Plastics tend to accumulate at higher elevations, where supratidal vegetation can trap debris beyond the area affected by tides [
114,
166,
169]. However, such patterns are not always consistent, partly due to the difficulty of identifying beach lines, which are often altered by the burial or displacement of litter [
98,
170]. Debris typically reaches the shore in successive, linear accumulation bands ranging from the most recent swash line to the high tide mark and a sequence of older deposition lines up to the storm berm [
171,
172]. As observed in the present study, storm events may flood the entire width of the beach, allowing water to reach the backshore and transport not only buoyant items but also previously buried materials that are exhumed [
88]. Similarly, a Danish study showed that the buoyancy of shotgun cartridges (including wads) plays a key role in their cross-shore distribution. While wads remain afloat and disperse more widely, cartridges tend to sink and accumulate sediments. They re-emerge only once the metallic parts have significantly corroded, unless they are fully embedded in the substrate [
146]. Smaller items exhibit greater spatial variability than larger ones, with distribution patterns strongly influenced by local conditions and seasonal fluctuations [
98,
166,
168,
173]. A multi-year analysis conducted along the Australian coast revealed that debris density and size increased from the waterline toward the backshore. This indicates that the backshore is a major deposition area, particularly for large items [
154]. According to some authors, this trend is due to the selective burial of smaller items in the backshore zone [
165]. Lastly, Olivelli and colleagues observed a significant correlation between debris density, wind, and Stokes drift [
154]: wind redistributes stranded litter across the different geomorphological zones of the beach, and stronger waves during storms drive debris further up to the backshore. These authors also emphasized that the debris presence in the lower beach sections is not solely attributable to direct deposition by beachgoers but also to marine and wind-driven transport preceding further aeolian redistribution inland. Overall, the present findings align with the prior evidence, further emphasizing the role of coastal morphology and environmental conditions in litter accumulation.
The Kruskal-Wallis test, non-metric multidimensional scaling (nMDS), and cluster analysis were applied to the normalized dataset and grouped by geomorphological zones, but no statistically significant results were obtained, likely due to the high variability in item categories and the generally low litter densities observed.