Risk of Expanded Polystyrene Ingestion by Climbing Perch Anabas testudineus
Round 1
Reviewer 1 Report
The purpose of the article submitted for review was to assess the feeding behavior of climbing perch in the presence of expanded polystyrene pellets in water and the risk of EPS ingestion.
The experiment revealed an effective defense mechanism of climbing perch to avoid the ingestion of expanded polystyrene pellets with size 2.5–3.5 mm. This mechanism realized by taste system and tactile reception of fish. The avoidance of plastic consumption by climbing perch facilitates its survival in waterbodies conditions contaminated with plastic waste.
The article is short but well-organized, properly substantive, at first the research may seem controversial, but in fact it proves the existence of natural defense systems of organisms against pollution. However, the article requires a few corrections:
1. I don't understand chapter 6. Patents
2. Please use a uniform record of citations. Please correct the citations on lines 56, 60 and 63. There is no need to use double references.
3. Figure 1 is not very precise. Requires correction.
4. Why is cm3 given in line 104? Please correct.
The publication is a valuable source of information and forms the basis for further analyzes.
Thank you for considering my opinion. I encourage the authors to continue working on improving the manuscript.
Author Response
Reviewer 1
The purpose of the article submitted for review was to assess the feeding behavior of climbing perch in the presence of expanded polystyrene pellets in water and the risk of EPS ingestion.
The experiment revealed an effective defense mechanism of climbing perch to avoid the ingestion of expanded polystyrene pellets with size 2.5–3.5 mm. This mechanism realized by taste system and tactile reception of fish. The avoidance of plastic consumption by climbing perch facilitates its survival in waterbodies conditions contaminated with plastic waste.
The article is short but well-organized, properly substantive, at first the research may seem controversial, but in fact it proves the existence of natural defense systems of organisms against pollution.
Reply to the Reviewer 1.
Dear Reviewer 1,
We would like to thank you for review and positive estimation of our work! We tried to correct the article according to your comments and made some editing.
However, the article requires a few corrections:
1. I don't understand chapter 6. Patents
Reply. We have not any patents. The Chapter has been deleted.
2. Please use a uniform record of citations. Please correct the citations on lines 56, 60 and 63. There is no need to use double references.
Reply. Thank you for your comment, it was corrected.
3. Figure 1 is not very precise. Requires correction.
Reply. We modified figure 1. The names of studied rivers, scale bar and North direction were added to the figure.
4. Why is cm3 given in line 104? Please correct.
Reply. It was corrected. We changed the size of tanks to their volume in liters.
The publication is a valuable source of information and forms the basis for further analyzes.
Reply. Thank you for the pleasant comment!
Thank you for considering my opinion. I encourage the authors to continue working on improving the manuscript.
Reply. We tried to do our best to improve the manuscript.
Reviewer 2 Report
Vietnam is one of the countries with serious marine plastic pollution. Floating marine debris is easily ingested by various marine organisms and passed along the food chain, posing a huge threat to the survival of marine organisms. Most of these marine debris enter the sea along with the river. Anabas Testudineus is one of the common fish in Southeast Asia. The paper uses this kind of fish as the research object to assess the severity of plastic pollution hazards. The experimental design of the paper is relatively reasonable, and the data reliability is strong. The revision suggestions are as follows:
1. Figure 1 should add a scale bar.
2. The Discussion section is recommended to be divided into several sections, and a theme should be summarized for each section.
3. The possibility and danger of some plastics spreading through the food chain related to Anabastestudineus can be added in the discussion section.
Author Response
Reviewer 2
Vietnam is one of the countries with serious marine plastic pollution. Floating marine debris is easily ingested by various marine organisms and passed along the food chain, posing a huge threat to the survival of marine organisms. Most of these marine debris enter the sea along with the river. Anabas Testudineus is one of the common fish in Southeast Asia. The paper uses this kind of fish as the research object to assess the severity of plastic pollution hazards. The experimental design of the paper is relatively reasonable, and the data reliability is strong.
Reply to the Reviewer 2.
Dear Reviewer 2,
We would like to thank you for review and positive estimation of our work! We tried to correct the article according to your comments and made some editing.
The revision suggestions are as follows:
1. Figure 1 should add a scale bar.
Reply. We modified figure 1. The names of studied rivers, scale bar and North direction were added to the figure.
2. The Discussion section is recommended to be divided into several sections, and a theme should be summarized for each section.
Reply. Thank you for your comment! We divided Discussion into several sections according to recommendation and made some corrections of the text.
3. The possibility and danger of some plastics spreading through the food chain related to Anabas testudineus can be added in the discussion section.
Reply. We added some information related to the risks and affects of plastics consumption on hydrobionts. Unfortunately, we have not enough information about the feeding features of Anabas testudineus in nature. We suppose that spreading through the food chain related to climbing perch needs additional studies.
Reviewer 3 Report
Title: Risk of expanded polystyrene ingestion by Climbing perch Anabas testudineus
1. Please check grammatical errors in manuscript,
2. Please cite some more references
Investigation of biological accumulation and eco-genotoxicity of bismuth oxide nanoparticle in fresh water snail Lymnaea luteola https://doi.org/10.1016/j.jksus.2021.101355
Detection of oxidative stress and DNA damage in freshwater snail Lymnea leuteola exposed to profenofos
https://doi.org/10.1007/s11783-018-1039-6
Sensitivity of freshwater pulmonate snail Lymnaea luteola L., to silver nanoparticles. DOI10.1016/j.chemosphere.2013.10.081
3. The discussion section needs significant improvement. Currently, it is diffuse and provides no real insights.
In summing up, my suggestion is that the paper be accepted after revision
Author Response
Reply to the Reviewer 3.
Dear Reviewer 3,
We would like to thank you for your review! We tried to correct the article according to your comments and made some editing.
Title: Risk of expanded polystyrene ingestion by Climbing perch Anabas testudineus
- Please check grammatical errors in manuscript,
Reply. Thank you for your comment. We edited the text through the manuscript.
- Please cite some more references
Investigation of biological accumulation and eco-genotoxicity of bismuth oxide nanoparticle in fresh water snail Lymnaea luteola https://doi.org/10.1016/j.jksus.2021.101355
Detection of oxidative stress and DNA damage in freshwater snail Lymnea leuteola exposed to profenofos
https://doi.org/10.1007/s11783-018-1039-6
Sensitivity of freshwater pulmonate snail Lymnaea luteola L., to silver nanoparticles. DOI10.1016/j.chemosphere.2013.10.081
Reply. We are sincerely grateful to you for sharing actual and informative investigations! We cited the article that described AgNP influence on the freshwater snail in our manuscript. We cited more articles to explain the connection between EPS and AgNP.
- The discussion section needs significant improvement. Currently, it is diffuse and provides no real insights.
Reply. Thank you for your comment! We divided the Discussion into several sections and made corrections of the text.
