Microbiological Mechanisms for Nitrogen Removal Using Anaerobic Fermentation Liquid from Spent Mushroom Substrates as a Carbon Source
Abstract
:1. Introduction
2. Material and Methods
2.1. Preparation of SMS Fermentation Liquid
2.2. SBR Device and Operation
2.3. High through Sequencing
2.4. Functional Gene Quantification
2.5. Extracellular Polymeric Substance Extraction and Determination
2.6. Analytical Techniques and Statistical Analysis
3. Results and Discussion
3.1. Composition and Microbial Community of Fermentation Broth
3.2. Denitrification Performance and COD Removal
3.3. Extracellular Polymeric Substance Composition
3.4. Absolute Abundance of Denitrification Functional Genes
3.5. Community Dynamics of Activated Sludge
3.6. Environmental and Economic Benefits
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviation
SMS | spent mushroom substrates |
C/N | carbon–nitrogen ratio |
EPS | extracellular polymeric substance |
SBR | sequencing batch reactor |
COD | chemical oxygen demand |
qPCR | quantitative polymerase chain reaction |
PN | extracellular protein |
PS | extracellular polysaccharides |
UV-vis | ultraviolet–visible spectrophotometer |
HPLC | high-performance liquid chromatography |
LC-MS | liquid chromatography mass spectrometry |
ICP-OES | inductively coupled plasma emission spectrometry |
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Operation Time (d) | C/N Ratio | Aeration Rate (m3/h) | Aeration Time (h) | Settling Time (min) | Fermentation Liquid Amount |
---|---|---|---|---|---|
1–5 | 13 | 20 | 6.5 | 30 | 5% |
6–10 | 6.5 | 20 | 7 | 20 | 10% |
11–15 | 6.5 | 20 | 7 | 10 | 15% |
16–20 | 6.5 | 20 | 7 | 10 | 20% |
21–25 | 6.5 | 20 | 7 | 10 | 25% |
26–30 | 6.5 | 20 | 7 | 10 | 30% |
Sequencing Type | Forward Primer | Reverse Primer | ||
---|---|---|---|---|
16S rDNA V3-V4 | 341F | ACTCCTACGGGAGGCAGCAG | 805R | GACTACHVGGGTATCTAATCC |
ITS ITS1-ITS2 | ITS1 | CTTGGTCATTTAGAGGAAGTAA | ITS2 | GCTGCGTTCTTCATCGATGC |
Index | Method/Instrument | Value |
---|---|---|
COD | Potassium dichromate method | 20,000 mg/L |
pH | pH meter | 6.19 |
Water | Karl Fischer | 96.5–98.5% |
Total protein | UV-vis | 0.4–0.7% |
Total sugar | UV-vis | 0.5–0.8% |
Acetic acid | HPLC | 0.33 mg/kg |
Lactate | HPLC | 0.07 mg/kg |
Glycollic acid | HPLC | 0.16 mg/kg |
l-alanine | LC-MS | 205.1 mg/kg |
l-leucine | LC-MS | 186.2 mg/kg |
l-valine | LC-MS | 117.8 mg/kg |
l-isoleucine | LC-MS | 116.3 mg/kg |
l-threonine | LC-MS | 79.4 mg/kg |
l-proline | LC-MS | 31.8 mg/kg |
l-phenylalanine | LC-MS | 73.5 mg/kg |
l-methionine | LC-MS | 25.5 mg/kg |
l-aspartate | LC-MS | 21.5 mg/kg |
l-glycine | LC-MS | 13.7 mg/kg |
l-tryptophan | LC-MS | 12.3 mg/kg |
K | ICP-OES | 1356.0 mg/kg |
Ca | ICP-OES | 974.0 mg/kg |
Mg | ICP-OES | 541.7 mg/kg |
Na | ICP-OES | 66.7 mg/kg |
Si | ICP-OES | 43.8 mg/kg |
Cu | ICP-OES | 21.9 mg/kg |
Fe | ICP-OES | 16.2 mg/kg |
Al | ICP-OES | 7.2 mg/kg |
Treatment | NirS (Average Copies) | NirK (Average Copies) |
---|---|---|
Control | 26,316.472 | 15,039.141 |
Fermentation broth | 6724.9544 | 3912.6366 |
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Chen, R.; Zhang, W.; Bi, X.; Jin, Y.; Yang, Y. Microbiological Mechanisms for Nitrogen Removal Using Anaerobic Fermentation Liquid from Spent Mushroom Substrates as a Carbon Source. Water 2023, 15, 3530. https://doi.org/10.3390/w15203530
Chen R, Zhang W, Bi X, Jin Y, Yang Y. Microbiological Mechanisms for Nitrogen Removal Using Anaerobic Fermentation Liquid from Spent Mushroom Substrates as a Carbon Source. Water. 2023; 15(20):3530. https://doi.org/10.3390/w15203530
Chicago/Turabian StyleChen, Ruihuan, Weihong Zhang, Xiaohui Bi, Yan Jin, and Yunlong Yang. 2023. "Microbiological Mechanisms for Nitrogen Removal Using Anaerobic Fermentation Liquid from Spent Mushroom Substrates as a Carbon Source" Water 15, no. 20: 3530. https://doi.org/10.3390/w15203530